New Method Development for Nanoparticle Extraction of Water-Soluble β-(1→3)-d-Glucan from Edible Mushrooms, Sparassis crispa and Phellinus linteus

Abstract

Sparassis crispa and Phellinus linteus are edible/medicinal mushrooms that have remarkably high contents of beta-(1-->3)-D-glucan, which acts as a biological response modifier, but difficulty in cultivating the fruiting bodies and extraction of beta-D-glucan have restricted detailed studies. Therefore, a novel process for nanoparticle extraction of Sparan, the beta-D-glucan from Sparassis crispa, and Phellin, the beta-D-glucan from Phellinus linteus, has been investigated using insoluble tungsten carbide as a model for nanoknife technology. This is the first report showing that the nanoknife method results in high yields of Sparan (70.2%) and Phellin (65.2%) with an average particle size of 150 and 390 nm, respectively. The extracted Sparan with beta-(1-->3) linkages showed a remarkably high water solubility of 90% even after 10 min of incubation at room temperature. Therefore, it is likely that this nanoknife method could be used to produce beta-D-glucan for food, cosmetic, and pharmaceutical industries.

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

“…The samples may be subjected to a previous step consisting of a methanolic extraction in order to remove phenolic compounds, monosaccharides, amino acids and other related molecules. The elimination of phenolic derivatives is successful [24], and increases the effectiveness of extraction [25]. Proteins can be removed by precipitation with trifluoroacetic acid (20%, w/v) or by treatment with the enzyme protease at 40 °C for 1 h (pH 7.5).…”

“…For instance, the (1→3),(1→6)-glucan isolated from Schizophyllum commune is termed schizophyllan [48]. Other mushrooms, such as Sparassis crispa, Phellinus linteus [25], and some of the genus Termitomyces [49,50], have also shown to possess β-D-glucans with different linkage patterns, depending on the fungal species.…”

Dietary Polysaccharides and Immune Modulation

“…New technologies to improve the quality of such biologics resulted in the development in 2009 of a new method for nanoparticle extraction of water-soluble β-glucans from medicinal mushrooms by the Park group (Park et al 2009) from South Korea for their use in the food, cosmetic, and pharmaceutical industries.…”

Therapeutic role of glucogalactan polysaccharide extracted from Agaricus bisporus on trimethyltin chloride induced neuropathy in rats

“…The samples were subjected to methanolic extraction in order to remove phenolic compounds, monosaccharides, amino acids and other related molecules. The elimination of phenolic derivatives was successful (Palacios et al, 2011), and it increased the effectiveness of the extraction (Park et al, 2009). Proteins were removed by precipitating with trifluoroacetic acid (20%, w/v) or treating with enzyme protease at 40°C for 1 h (pH 7.5).…”