New Method for the Extraction of Single-Cell Oils from Wet Oleaginous Microbial Biomass: Efficiency, Oil Characterisation and Energy Assessment

Neto, Carlos José Dalmas; Sydney, Eduardo Bittencourt; Candeo, Esteffany de Souza; Souza, E. B. S. de; Camargo, D.; Sydney, Alessandra Cristine Novak; Carvalho, Júlio César de; Letti, Luiz Alberto Júnior; Pandey, Ashok; Soccol, Carlos Ricardo

doi:10.1007/s12649-019-00705-x

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…According to the literature, oleaginous yeasts are rich in fatty acids from the 16 and 18 carbon atoms [102]. A similar profile has been reported for Brown (1996) [81]; Chi (2008) [29]; Kang (2006) [103]; Enshaeieh (2013 and [96,97]; Wang (2017) [104]; Dalmas Neto (2019) [92]. The lipids accumulated by oleaginous yeasts are mainly composed of long chain fatty acids, in particular oleic acid (C18: 1), palmitic acid (C16), linoleic acid (C18: 2) and stearic acid (C18) similar to the composition of vegetable oils and can be converted into biodiesel by enzymatic or inorganic catalysis [105][106][107][108].…”

Section: Fatty Acid Profilesupporting

confidence: 62%

“…The efficient extraction of lipids and the culture medium have the greatest impact on the quantity of microbial lipids and are considered as the main limiting factors for large-scale culture [91,92]. Thus, for certain species of marine yeast cultivated under the same conditions as this study (substrate: glucose; temperature) and the method of lipid extraction (Folch), the lipid contents vary according to the species.…”

Section: Lipid Contentmentioning

confidence: 99%

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Molecular Identification and Biochemical Characterization of Novel Marine Yeast Strains with Potential Application in Industrial Biotechnology

et al. 2022

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Cell-based agriculture is an emerging and attractive alternative to produce various food ingredients. In this study, five strains of marine yeast were isolated, molecularly identified and biochemically characterized. Molecular identification was realized by sequencing the DNA ITS1 and D1/D2 region, and sequences were registered in GenBank as Yarrowia lipolytica YlTun15, Rhodotorula mucilaginosa RmTun15, Candida tenuis CtTun15, Debaryomyces hansenii DhTun2015 and Trichosporon asahii TaTun15. Yeasts showed protein content varying from 26% (YlTun15) to 40% (CtTun15 and DhTun2015), and essential amino acids ranging from 38.1 to 64.4% of the total AAs (CtTun15-YlTun15, respectively). Lipid content varied from 11.15 to 37.57% with substantial amount of PUFA (>12% in RmTun15). All species had low levels of Na (<0.15 mg/100 g) but are a good source of Ca and K. Yeast cytotoxic effect was investigated against human embryonic kidney cells (HEK 293); results showed improved cell viability with all added strains, indicating safety of the strains used. Based on thorough literature investigation and yeast composition, the five identified strains could be classified not only as oleaginous yeasts but also as single cell protein (SCP) (DhTun2015 and CtTun15) and single cell oil (SCO) (RmTun15, YlTun15 and TaTun15) producers; and therefore, they represent a source of alternative ingredients for food, feed and other sectors.

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Section: Fatty Acid Profilesupporting

confidence: 62%

Section: Lipid Contentmentioning

confidence: 99%

Molecular Identification and Biochemical Characterization of Novel Marine Yeast Strains with Potential Application in Industrial Biotechnology

et al. 2022

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“…Conventionally, the extraction of lipids from microbes is conducted using Soxhlet or Folch techniques (Neto et al, 2019). These techniques firstly involve biomass recovery from the broth through centrifugation and subsequent drying before solvent extraction.…”

Section: Considerations For Sco Recovery Methodsmentioning

confidence: 99%

“…Oil recovery from dry biomass is considered to be efficient, however, there are economical and safety shortcomings involved. Drying of biomass involves extensive energy use and may also lead to substantial compound degradation due to high reaction temperatures (Xu et al, 2011;Neto et al, 2019). Soxhlet extraction requires extended durations of extraction and high solvent quantities (Somashekar et al, 2001).…”

Section: Considerations For Sco Recovery Methodsmentioning

confidence: 99%

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

et al. 2021

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Microbial lipids, also known as single-cell oils (SCOs), are highly attractive feedstocks for biodiesel production due to their fast production rates, minimal labor requirements, independence from seasonal and climatic changes, and ease of scale-up for industrial processing. Among the SCO producers, the less explored filamentous fungi (molds) exhibit desirable features such as a repertoire of hydrolyzing enzymes and a unique pellet morphology that facilitates downstream harvesting. Although several oleaginous filamentous fungi have been identified and explored for SCO production, high production costs and technical difficulties still make the process less attractive compared to conventional lipid sources for biodiesel production. This review aims to highlight the ability of filamentous fungi to hydrolyze various organic wastes for SCO production and explore current strategies to enhance the efficiency and cost-effectiveness of the SCO production and recovery process. The review also highlights the mechanisms and components governing lipogenic pathways, which can inform the rational designs of processing conditions and metabolic engineering efforts for increasing the quality and accumulation of lipids in filamentous fungi. Furthermore, we describe other process integration strategies such as the co-production with hydrogen using advanced fermentation processes as a step toward a biorefinery process. These innovative approaches allow for integrating upstream and downstream processing units, thus resulting in an efficient and cost-effective method of simultaneous SCO production and utilization for biodiesel production.

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“…After several cycles of evaporation, condensation and percolation of solvent through the biomass sample, the flask containing a mixture of solvent and extracted lipids is taken out to recover the crude lipids after vaporizing the solvent using a rota-evaporator [114]. Dalmas Neto et al [115] evaluated the performance of three different solvents, hexane, chloroform and chloroform/methanol (2:1), for the extraction of lipids from dried R. toruloides DEBB 5533 using Soxhlet extraction. They discovered the amounts of lipids obtained were comparable with 46.1, 44.7 and 43.2% for hexane, chloroform/methanol and chloroform, respectively.…”

Section: Soxhlet Extractionmentioning

confidence: 99%

Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

et al. 2021

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The production of lipids from oleaginous yeasts involves several stages starting from cultivation and lipid accumulation, biomass harvesting and finally lipids extraction. However, the complex and relatively resistant cell wall of yeasts limits the full recovery of intracellular lipids and usually solvent extraction is not sufficient to effectively extract the lipid bodies. A pretreatment or cell disruption method is hence a prerequisite prior to solvent extraction. In general, there are no recovery methods that are equally efficient for different species of oleaginous yeasts. Each method adopts different mechanisms to disrupt cells and extract the lipids, thus a systematic evaluation is essential before choosing a particular method. In this review, mechanical (bead mill, ultrasonication, homogenization and microwave) and nonmechanical (enzyme, acid, base digestions and osmotic shock) methods that are currently used for the disruption or permeabilization of oleaginous yeasts are discussed based on their principle, application and feasibility, including their effects on the lipid yield. The attempts of using conventional and “green” solvents to selectively extract lipids are compared. Other emerging methods such as automated pressurized liquid extraction, supercritical fluid extraction and simultaneous in situ lipid recovery using capturing agents are also reviewed to facilitate the choice of more effective lipid recovery methods.

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New Method for the Extraction of Single-Cell Oils from Wet Oleaginous Microbial Biomass: Efficiency, Oil Characterisation and Energy Assessment

Cited by 11 publications

References 18 publications

Molecular Identification and Biochemical Characterization of Novel Marine Yeast Strains with Potential Application in Industrial Biotechnology

Molecular Identification and Biochemical Characterization of Novel Marine Yeast Strains with Potential Application in Industrial Biotechnology

The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production

Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

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