BACKGROUND: Peri-implantitis is mainly caused by the activity of periodontal pathogens with insufficient oral hygiene, which causes the disintegration of intraosseous dental implants against the background of chronic inflammation in peri-implant tissues. The correct techniques in the post-prosthetic stage in patients with dental implants include medical examination with regular professional oral hygiene, which is complicated by low patient compliance. In this regard, there are high requirements for the level of individual oral hygiene and for the effectiveness of local hygiene products, among which dental rinses occupy an important place. As rinses, it is possible to use pine-containing substances, particularly a complex of oil-essential fir Solagift of a Siberian company (Tomsk).
AIM: To conduct a microbiological study of the sensitivity of periodontopathogens and Candida albicans to the oil-essential complex of Siberian fir of different concentrations.
MATERIAL AND METHODS: A number of periodontopathogens and C. albicans were cultured in the presence of a complex of oilessential Siberian fir in the following proportions: 1:5, 1:10, and 1:15. Incubation for up to 3 days was conducted in the Reverse Spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density of the culture (OD) at a wavelength of =850 nm. The optical density was measured in McFarland units (Mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontopathogens: adaptive (lag phase), exponential (log phase), stationary, and dying. The following clinical isolates of microorganisms were used: Streptococcus constellatus, Staphylococcus aureus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and C. albicans.
RESULTS: Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5 by 13.727.1% (A. actinomycetemcomitans), 18.362.0% (F. nucleatum), 30.056.4% (S. aureus), and 19.274.1% (S. constellatus). The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%.
CONCLUSION. Joint cultivation of periodontopathogens with the oilether complex of Siberian fir reduces the OD of cultures of clinical isolates when breeding coniferous substance 1:151:5. The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the OD of the culture of fungi in the microbiological experiment by 29.8%. The bacteriostatic effectiveness of the Siberian fir oil-ether complex significantly exceeds the effect of the herbal preparation Stomatophyte.