New Polymer Passive Sampler for Sensitive Biomonitoring of Lipid-Rich Matrices

Abstract

16The feasibility of passive sampling biological matrices to quantify contaminants has been 17 demonstrated using polydimethylsiloxane (PDMS). PDMS has, however, low sorptive capacity for 18 hydrophobic compounds (with K lipid-PDMS ∼30-40), and increasing the sampler volume and thus 19 chemical mass transfer is not feasible due to concomitant lipid transfer. We therefore developed 20 new polymers by graft polymerization from PDMS substrates, and evaluated the mechanism and 21 kinetics of lipid transfer. K lipid-polymer was significantly improved to 6.7 ± 0.53 for dioxins and 0.78 ± 22 0.15 for PCBs using poly(tertiary-butyl methacrylate) (PtBuMA) chain grafts. Consistent with this, 23PtBuMA solubility of selected dioxins was 6-10 times higher compared to PDMS. Lipid transfer 24 followed a swelling process, which was rapid (t 95% =20-72 hours), independent of tissue lipid content 25 and proportional to polymer sampler volume. The new PtBuMA polymer offers new opportunities 26 for sensitive, rapid biomonitoring of PBTs and possibly also less stable neutral hydrophobic 27 compounds in biota and food.

Equilibrium Passive Sampling of POP in Lipid-Rich and Lean Fish Tissue: Quality Control Using Performance Reference Compounds

“…In-tissue EPS excludes the laborious determination of lipid content with related uncertainties and is analytically easier to execute because the matrix effect is diminished compared to solvent extraction of tissue. Co-extraction of lipid is limited to ∼6 mg g −1 PDMS sampler, and absorbed lipid showed no effect on sampler uptake. , Recently, a new polymer passive sampler with considerable higher sorption capacity was proposed, but wide suitability for in-tissue EPS is doubtful due to the associated longer equilibrium times. As alternative to PDMS used in this study, LDPE could be applied for in-tissue EPS.…”

“…Co-extraction of lipid is limited to ∼6 mg g −1 PDMS sampler, and absorbed lipid showed no effect on sampler uptake. 18,27 Recently, a new polymer passive sampler with considerable higher sorption capacity was proposed, 28 but wide suitability for in-tissue EPS is doubtful due to the associated longer equilibrium times. As alternative to PDMS used in this study, LDPE could be applied for in-tissue EPS.…”