New Polymeric Composites Based on Two-Dimensional Nanomaterials for Biomedical Applications

Pires, Laura S; Magalhães, Fernão D.; Pinto, Artur M.

doi:10.3390/polym14071464

Cited by 7 publications

(4 citation statements)

References 135 publications

(159 reference statements)

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“…Then, Rapa, as a potent immunosuppressive drug with anti-proliferation and anti-inflammation effects, was loaded on the Ti 3 C 2 nanosheets using the electrostatic self-assembly method. Due to the negative charge on the surface of Ti 3 C 2 nanosheets and the positive charge on Rapa molecules, a high drug loading capacity could be achieved ( Huang et al, 2022 ; Korupalli et al, 2022 ; Pires et al, 2022 ). The Ti 3 C 2 nanosheets and Rapa-Ti 3 C 2 nanosheets thus prepared have abundant functional groups on their surfaces, which can be easily dispersed in PBS ( Supplementary Figures S1A,B ).…”

Section: Resultsmentioning

confidence: 99%

Two-dimensional ultrathin Ti3C2 MXene nanosheets coated intraocular lens for synergistic photothermal and NIR-controllable rapamycin releasing therapy against posterior capsule opacification

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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Posterior capsule opacification (PCO) is one of the most frequent late-onset complications after cataract surgery. Several kinds of drug-eluting intraocular lenses (IOL) were designed for sustainable drug release to suppress ocular inflammation, the proliferation of lens epithelial cells (LECs) and the development of PCO after cataract surgery. Despite previous advances in this field, the drug-loaded IOLs were limited in ocular toxicity, insufficient drug-loading capacity, and short release time. To prevent PCO and to address these drawbacks, a novel drug-loaded IOL (Rapa@Ti3C2-IOL), prepared from two-dimensional ultrathin Ti3C2 MXene nanosheets and rapamycin (Rapa), was fabricated with a two-step spin coating method in this study. Rapa@Ti3C2 was prepared via electrostatic self-assembly of Ti3C2 and Rapa, with a loading capacity of Rapa at 92%. Ti3C2 was used as a drug delivery reservoir of Rapa. Rapa@Ti3C2-IOL was designed to have the synergistic photothermal and near infrared (NIR)-controllable drug release property. As a result, Rapa@Ti3C2-IOL exhibited the advantages of simple preparation, high light transmittance, excellent photothermal conversion capacity, and NIR-controllable drug release behavior. The Rapa@Ti3C2 coating effectively eliminated the LECs around Rapa@Ti3C2-IOL under a mild 808-nm NIR laser irradiation (1.0 W/cm−2). Moreover, NIR-controllable Rapa release inhibited the migration of LECs and suppressed the inflammatory response after photothermal therapy in vitro. Then, Rapa@Ti3C2-IOL was implanted into chinchilla rabbit eyes, and the effectiveness and biocompatibility to prevent PCO were evaluated for 4 weeks. The Rapa@Ti3C2-IOL implant exhibited excellent PCO prevention ability with the assistance of NIR irradiation and no obvious pathological damage was observed in surrounding healthy tissues. In summary, the present study offers a promising strategy for preventing PCO via ultrathin Ti3C2 MXene nanosheet-based IOLs with synergistic photothermal and NIR-controllable Rapa release properties.

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Section: Resultsmentioning

confidence: 99%

Two-dimensional ultrathin Ti3C2 MXene nanosheets coated intraocular lens for synergistic photothermal and NIR-controllable rapamycin releasing therapy against posterior capsule opacification

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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“…Moreover, the zeta potential of rGNP@ is lower than that of non-deposited ION, indicating that the former have higher water stability (Figure S1B). Such features were preferred to ensure biocompatibility, since poor water dispersability has been associated with a lack of biodegradation, bioaccumulation, and toxicity of nanomaterials [36,37] , while large particles (above 200 nm) are more often phagocytosed than internalized [38] .…”

Section: Synthesis Of Magnetized Particles and Magnetic Pcl Compositesmentioning

confidence: 99%

3D printed magneto-active microfiber scaffolds for remote stimulation of 3Din vitroskeletal muscle models

Servin

Dahri

Meneses

et al. 2023

Preprint

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Tunable culture platforms that guide cellular organization and mechanically stimulate skeletal muscle development are still unavailable due to limitations in biocompatibility and actuation triggered without contact. This study reports the rational design and fabrication of magneto-active microfiber meshes with controlled hexagonal microstructures via melt electrowriting (MEW) of a thermoplastic/graphene/iron oxide composite. In situ deposition of iron oxide nanoparticles on oxidized graphene yielded homogeneously dispersed magnetic particles with sizes above 0.5 micrometer and low aspect ratio, preventing cellular internalization and toxicity. With these fillers, homogeneous magnetic composites with very high magnetic filler content (up to 10 wt.%) were obtained and successfully processed in a solvent-free manner for the first time. MEW of magnetic composites enabled the skeletal muscle-inspired design of hexagonal scaffolds with tunable fiber diameter, reconfigurable modularity, and zonal distribution of magneto-active and nonactive material. Importantly, the hexagonal microstructures displayed elastic deformability under tension, mitigating the mechanical limitations due to high filler content. External magnetic fields below 300 mT were sufficient to trigger out-of-plane reversible deformation leading to effective end-to-end length decrease up to 17%. Moreover, C2C12 myoblast culture on 3D Matrigel/collagen/MEW scaffolds showed that the presence of magnetic particles in the scaffolds did not significantly affect viability after 8 days with respect to scaffolds without magnetic filler. Importantly, in vitro culture demonstrated that myoblasts underwent differentiation at similar rates regardless of the presence of magnetic filler. Overall, these innovative microfiber scaffolds were proven as a magnetically deformable platform suitable for dynamic culture of skeletal muscle with potential for in vitro disease modeling.

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“…Moreover, the zeta potential of rGNP@ is lower than that of non-deposited ION, indicating that the former had higher water stability (Figure S1B, Supporting Information). Such features were preferred to ensure biocompatibility, since poor water dispersability has been associated with a lack of biodegradation, bioaccumulation, and toxicity of nanomaterials, [29,30] while large particles (>200 nm) are more often phagocytosed than internalized. [31] Furthermore, particle morphology has been identified as a mechanism for ION cytotoxicity.…”

Section: Synthesis Of Magnetized Particles and Magnetic Polycaprolact...mentioning

confidence: 99%

3D Printed Magneto‐Active Microfiber Scaffolds for Remote Stimulation and Guided Organization of 3D In Vitro Skeletal Muscle Models

Cedillo‐Servin,

Dahri,

Meneses

et al. 2023

Small

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This work reports the rational design and fabrication of magneto‐active microfiber meshes with controlled hexagonal microstructures via melt electrowriting (MEW) of a magnetized polycaprolactone‐based composite. In situ iron oxide nanoparticle deposition on oxidized graphene yields homogeneously dispersed magnetic particles with sizes above 0.5 µm and low aspect ratio, preventing cellular internalization and toxicity. With these fillers, homogeneous magnetic composites with high magnetic content (up to 20 weight %) are obtained and processed in a solvent‐free manner for the first time. MEW of magnetic composites enabled the creation of skeletal muscle‐inspired design of hexagonal scaffolds with tunable fiber diameter, reconfigurable modularity, and zonal distribution of magneto‐active and nonactive material, with elastic tensile deformability. External magnetic fields below 300 mT are sufficient to trigger out‐of‐plane reversible deformation. In vitro culture of C2C12 myoblasts on three‐dimensional (3D) Matrigel/collagen/MEW scaffolds showed that microfibers guided the formation of 3D myotube architectures, and the presence of magnetic particles does not significantly affect viability or differentiation rates after 8 days. Centimeter‐sized skeletal muscle constructs allowed for reversible, continued, and dynamic magneto‐mechanical stimulation. Overall, these innovative microfiber scaffolds provide magnetically deformable platforms suitable for dynamic culture of skeletal muscle, offering potential for in vitro disease modeling.

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New Polymeric Composites Based on Two-Dimensional Nanomaterials for Biomedical Applications

Cited by 7 publications

References 135 publications

Two-dimensional ultrathin Ti3C2 MXene nanosheets coated intraocular lens for synergistic photothermal and NIR-controllable rapamycin releasing therapy against posterior capsule opacification

Two-dimensional ultrathin Ti3C2 MXene nanosheets coated intraocular lens for synergistic photothermal and NIR-controllable rapamycin releasing therapy against posterior capsule opacification

3D printed magneto-active microfiber scaffolds for remote stimulation of 3Din vitroskeletal muscle models

3D Printed Magneto‐Active Microfiber Scaffolds for Remote Stimulation and Guided Organization of 3D In Vitro Skeletal Muscle Models

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