New reversed-phase liquid chromatographic method to detect aflatoxins in food and feed with cyclodextrins as fluorescence enhancers added to the eluent

Chiavaro, Emma; Dall’Asta, Chiara; Galaverna, Gianni; Biancardi, Alberto; Gambarelli, E; Dossena, Arnaldo; Marchelli, Rosangela

doi:10.1016/s0021-9673(01)01300-0

Cited by 95 publications

(50 citation statements)

References 19 publications

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“…A post-column derivatisation method that seems analytically equivalent to iodination and bromination is the photochemical one: it is based on the formation of hemiacetals of AFB1 and AFG1 as the effect of the irradiation of the HPLC column eluate by a UV light (Joshua, 1993;Waltking & Wilson, 2006). A method based on the formation of an inclusion complex between aflatoxins and cyclodextrins (CDs) has been recently developed (Chiavaro et al, 2001): specific CDs are added to mobile phase (water-methanol) including aflatoxins in their cyclic structure, enhancing AFB1 and AFG1 fluorescence (Aghamohammadi & Alizadeh, 2007). The introduction of mass spectrometry and the subsequent coupling of liquid chromatography to this very efficient system of detection have resulted in the development of many LC-MS or LC-MS/MS methods for aflatoxin analysis.…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Aflatoxins: Their Measure and Analysis

Manetta¹

2011

Aflatoxins - Detection, Measurement and Control

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Section: Chromatographic Methodsmentioning

confidence: 99%

Aflatoxins: Their Measure and Analysis

Manetta¹

2011

Aflatoxins - Detection, Measurement and Control

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“…However, aflatoxins have a weak native fluorescence, which can be enhanced by preor post-column derivatization. Addition of the cyclodextrin to the LC eluent has been proved to highly enhance cyclodextrin fluorescence (Chiavaro et al, 2001). The method usually requires extraction and clean-up procedures, which enhance sensitivity and specificity.…”

Section: Aflatoxinsmentioning

confidence: 99%

Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry

Sforza

Dall’Asta

Marchelli

2005

Mass Spectrometry Reviews

196

104

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Mycotoxins are fungal toxins produced by molds, which occur universally in food and feed derivatives, and are produced under certain environmental conditions in the field before harvest, post‐harvest, during storage, processing, and feeding. Mycotoxin contamination is one of the most relevant and worrisome problem concerning food and feed safety because it can cause a variety of toxic acute and chronic effects in human and animals. In this review we report the use of mass spectrometry in connection with chromatographic techniques for mycotoxin determination by considering separately the most diffuse class of mycotoxins: patulin, aflatoxins, ochratoxin A, zearalenone, trichothecenes, and fumonisins. Although the selectivity of mass spectrometry is unchallenged if compared to common GC and LC detection methods, accuracy, precision, and sensitivity may be extremely variable concerning the different mycotoxins, matrices, and instruments. The sensitivity issue may be a real problem in the case of LC/MS, where the response can be very different for the different ionization techniques (ESI, APCI, APPI). Therefore, when other detection methods (such as fluorescence or UV absorbance) can be used for the quantitative determination, LC/MS appears to be only an outstanding confirmatory technique. In contrast, when the toxins are not volatile and do not bear suitable chromophores or fluorophores, LC/MS appears to be the unique method to perform quantitative and qualitative analyses without requiring any derivatization procedure. The problem of exact quantitative determination in GC/MS and LC/MS methods is particularly important for mycotoxin determination in food, given the high variability of the matrices, and can be solved only by the use of isotopically labeled internal standards or by the use of ionization interfaces able to lower matrix effects and ion suppressions. When the problems linked to inconstant ionization and matrix effects will be solved, only MS detectors will allow to simplify more and more the sample preparation procedures and to avoid clean‐up procedures, making feasible low‐cost, high‐throughput determination of mycotoxins in many different food matrices. © 2005 Wiley Periodicals, Inc.

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“…Since the use of cyclodextrins (CDs) as fluorescence enhancers for aflatoxin detection is widely reported in the literature, and succinyl--CD is considered to be the most efficient fluorescence enhancer for AFM1 [32], [33], a new titration curve was measured by using succinyl--CD at 5 mM ratio. Fig.…”

Section: Portable Fluorometer For the Rapid Detection In Milk Of Tmentioning

confidence: 99%

Eat-by-light fiber-optic and micro-optic devices for food quality and safety assessment

et al. 2007

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Abstract-A selection is presented of fiber-optic and micro-optic devices that have been designed and tested for guaranteeing the quality and safety of typical foods, such as extra virgin olive oil, beer, and milk. Scattered colorimetry is used to authenticate various types of extra virgin olive oil and beer, while a fiber-opticbased device for UV-VIS-NIR absorption spectroscopy is exploited in order to obtain the hyperspectral optical signature of olive oil. This is done not only for authentication purposes, but also so as to correlate the spectral data with the content of fatty acids, which are important nutritional factors. A micro-optic sensor for the detection of olive oil aroma that is capable of distinguishing different ageing levels of extra virgin olive oil is also presented. It shows effective potential for acting as a smart cap of bottled olive oil in order to achieve a nondestructive olfactory perception of oil ageing. Lastly, a compact portable fluorometer for the rapid monitoring of the carcinogenic M1 aflatoxin in milk, is experimented.Index Terms-Absorption spectroscopy, aflatoxins, beer, fluorescence spectroscopy, food authentication, milk, olive oil, scattered colorimetry. I. BETTER FOOD FOR BETTER HEALTHT HE quality and safety of the food we eat attracts a great deal of publicity, and is high on the list of public concerns. This highly emotional issue leads to a more "aware" con- Manuscript received July 20, 2007; accepted February 14, 2008. Published July 16, 2008. This work was supported by the EU SM&T Programme, under Contract SMT4-CT972157 "OPTIMO"; Regione Toscana, ITT "CARABIOTEC"; MIUR-FIRB, under Contract RBNE01KZZM "BIOSENS"; EU FP6 Network of Excellence Contract 003887 "NEMO"; CNR Short Term Mobility Program; DWTC-IAP, FWO Vlaanderen and the OZR of the Vrije Universiteit Brussel; and Regione Sicilia, Assessorato Agricoltura e Foreste, Servizio IX , Palermo. The work of H. Ottevaere was supported by the Flemish Fund for Scientific Research (FWO) under the "Postdoctoraal Onderzoeker" Fellowship. The associate editor coordinating the review of this paper and approving it for publication was Prof. Brian Culshaw.A. G. Mignani, L. Ciaccheri, C. Cucci, and A. A. Mencaglia are with the CNR-IFAC, Sesto Fiorentino (FI), Italy (e-mail: a.g.mignani@ifac.cnr.it).A. Cimato and C. . We live in a global marketplace, and we eat a great variety of foods from many different countries. Many fruits, vegetables, and dairy products now on the market were not available ten years ago. These are grown using a variety of production practices, from small organic operations to large-scale mechanized farms. On the one hand, produce buyers want to be reassured that farmers are taking reasonable steps to ensure that the produce delivered is safe and free from human pathogenic bacteria and mycotoxins. On the other hand, consumers and food handlers (restaurateurs, retailers, etc.) need to know that the food which they eat and serve is safe and healthy.In addition to conventional analytical techniques, new instruments...

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New reversed-phase liquid chromatographic method to detect aflatoxins in food and feed with cyclodextrins as fluorescence enhancers added to the eluent

Cited by 95 publications

References 19 publications

Aflatoxins: Their Measure and Analysis

Aflatoxins: Their Measure and Analysis

Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry

Eat-by-light fiber-optic and micro-optic devices for food quality and safety assessment

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