New Strategies in the Design of Nanomedicines to Oppose Uptake by the Mononuclear Phagocyte System and Enhance Cancer Therapeutic Efficacy

Zhou, Yiming; Dai, Zhifei

doi:10.1002/asia.201800149

Cited by 61 publications

(37 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By fluorescence analysis, we observed the highest accumulation of EVs in the liver (Fig. 6d, e), which was to be expected, as the liver is a highly irrigated organ where most of the nanoparticles tend to accumulate, including EVs [13,60]. Importantly, we observed no significant differences between the distribution of EV-AuNPs and control EVs, indicating that AuNPs did not affect systemic distribution (Fig.…”

Section: Tracking Double-labeled B16f10-ev Accumulation In Metastaticsupporting

confidence: 74%

Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Lara¹,

Palma-Florez²,

Salas-Huenuleo³

et al. 2020

J Nanobiotechnol

View full text Add to dashboard Cite

Background: Extracellular vesicles (EVs) have shown great potential for targeted therapy, as they have a natural ability to pass through biological barriers and, depending on their origin, can preferentially accumulate at defined sites, including tumors. Analyzing the potential of EVs to target specific cells remains challenging, considering the unspecific binding of lipophilic tracers to other proteins, the limitations of fluorescence for deep tissue imaging and the effect of external labeling strategies on their natural tropism. In this work, we determined the cell-type specific tropism of B16F10-EVs towards cancer cell and metastatic tumors by using fluorescence analysis and quantitative gold labeling measurements. Surface functionalization of plasmonic gold nanoparticles was used to promote indirect labeling of EVs without affecting size distribution, polydispersity, surface charge, protein markers, cell uptake or in vivo biodistribution. Double-labeled EVs with gold and fluorescent dyes were injected into animals developing metastatic lung nodules and analyzed by fluorescence/computer tomography imaging, quantitative neutron activation analysis and gold-enhanced optical microscopy. Results: We determined that B16F10 cells preferentially take up their own EVs, when compared with colon adenocarcinoma, macrophage and kidney cell-derived EVs. In addition, we were able to detect the preferential accumulation of B16F10 EVs in small metastatic tumors located in lungs when compared with the rest of the organs, as well as their precise distribution between tumor vessels, alveolus and tumor nodules by histological analysis. Finally, we observed that tumor EVs can be used as effective vectors to increase gold nanoparticle delivery towards metastatic nodules.

show abstract

Section: Tracking Double-labeled B16f10-ev Accumulation In Metastaticsupporting

confidence: 74%

Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Lara¹,

Palma-Florez²,

Salas-Huenuleo³

et al. 2020

J Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…In principle, cationic nanoparticles have higher affinity for macrophages than neutral and anionic nanomaterials. 33 Attachment of surface protein, such as opsonins may result in opsonization and subsequent phagocytosis. The negative zeta potential of the AGuIX@PS@KDKPPR nanoparticle characterizes its surface charge, which is essential for preventing opsonization in vivo and contributes to successfully achieve an effective targeting.…”

Section: Results and Discussion Physicochemical Characterization And mentioning

confidence: 99%

<p>Multiscale Selectivity and in vivo Biodistribution of NRP-1-Targeted Theranostic AGuIX Nanoparticles for PDT of Glioblastoma</p>

Gries¹,

Thomas²,

Daouk³

et al. 2020

IJN

View full text Add to dashboard Cite

Background: Local recurrences of glioblastoma (GBM) after heavy standard treatments remain frequent and lead to a poor prognostic. Major challenges are the infiltrative part of the tumor tissue which is the ultimate cause of recurrence. The therapeutic arsenal faces the difficulty of eradicating this infiltrating part of the tumor tissue while increasing the targeting of tumor and endogenous stromal cells such as angiogenic endothelial cells. In this aim, neuropilin-1 (NRP-1), a transmembrane receptor mainly overexpressed by endothelial cells of the tumor vascular system and associated with malignancy, proliferation and migration of GBM, highlighted to be a relevant molecular target to promote the anti-vascular effect of photodynamic therapy (VTP). Methods: The multiscale selectivity was investigated for KDKPPR peptide moiety targeting NRP-1 and a porphyrin molecule as photosensitizer (PS), both grafted onto original AGuIX design nanoparticle. AGuIX nanoparticle, currently in Phase II clinical trials for the treatment of brain metastases with radiotherapy, allows to achieve a real-time magnetic resonance imaging (MRI) and an accumulation in the tumor area by EPR (enhanced permeability and retention) effect. Using surface-plasmon resonance (SPR), we evaluated the affinities of KDKPPR and scramble free peptides, and also peptides-conjugated AGuIX nanoparticles to recombinant rat and human NRP-1 proteins. For in vivo selectivity, we used a cranial window model and parametric maps obtained from T2*-weighted perfusion MRI analysis. Results: The photophysical characteristics of the PS and KDKPPR molecular affinity for recombinant human NRP-1 proteins were maintained after the functionalization of AGuIX nanoparticle with a dissociation constant of 4.7 μM determined by SPR assays. Cranial window model and parametric maps, both revealed a prolonged retention in the vascular system of human xenotransplanted GBM. Thanks to the fluorescence of porphyrin by noninvasive imaging and the concentration of gadolinium evaluated after extraction of organs, we checked the absence of nanoparticle in the brains of tumor-free animals and highlighted elimination by renal excretion and hepatic metabolism. Conclusion: Post-VTP follow-ups demonstrated promising tumor responses with a prolonged delay in tumor growth accompanied by a decrease in tumor metabolism.

show abstract

“…This will slightly change the secondary structure of the proteins and lead to the formation of both soft and hard protein corona. [22][23][24] Formation of protein corona on nanomaterials will affect the surface of nanomaterials and their subsequent interaction with biological molecules (biodistribution and penetrance) and cellular compartments. 18,25 The composition, properties and formation of protein corona are influenced by physiochemical properties (size, shape, surface area, charge, chemical composition, functionalization, colloidal stability, porosity, etc.)…”

Section: (I) Interaction Of Nanomaterials With Serum Proteinsmentioning

confidence: 99%

Nanomaterials for Nanotheranostics: Tuning Their Properties According to Disease Needs

et al. 2020

View full text Add to dashboard Cite

Nanotheranostics is one of the biggest scientific breakthroughs in nanomedicine. Most of the currently available diagnosis and therapies are invasive, time-consuming and associated with severe toxic side effects. Nanotheranostics, on the other hand, has the potential to bridge this gap by harnessing the capabilities of nanotechnology and nanomaterials for combined therapeutics and diagnostics with markedly enhanced efficacy. However, nanomaterial applications in nanotheranostics are still in its infancy. This is due to the fact that each disease has a particular microenvironment with well-defined characteristics, which promotes deeper selection criteria of nanomaterials to meet the disease needs. In this review, we have outlined how nanomaterials are designed and tailored for nanotheranostics of cancer, and other diseases such as neurodegenerative, autoimmune (particularly on rheumatoid arthritis) and cardiovascular diseases. The penetrability and retention of a nanomaterial in the biological system, the therapeutic strategy used and the imaging mode selected are some of the aspects discussed for each disease. The specific properties of the nanomaterials in terms of feasibility, physicochemical challenges, progress in clinical trials, its toxicity and their future application on translational medicine are addressed. Our review meticulously and critically examines the applications of nanotheranostics with various nanomaterials, including graphene, across several diseases; offering a broader perspective of this emerging field.

show abstract

New Strategies in the Design of Nanomedicines to Oppose Uptake by the Mononuclear Phagocyte System and Enhance Cancer Therapeutic Efficacy

Cited by 61 publications

References 104 publications

Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors

<p>Multiscale Selectivity and in vivo Biodistribution of NRP-1-Targeted Theranostic AGuIX Nanoparticles for PDT of Glioblastoma</p>

Nanomaterials for Nanotheranostics: Tuning Their Properties According to Disease Needs

Contact Info

Product

Resources

About