Newcastle Disease Virus

Dey, Sohini; Pathak, D. C.; Debnath, Ashis; Narayan, Raj; Rahul, .; D’Silva, Ajai Lawrence; Chellappa, Madhan Mohan

doi:10.1007/978-981-13-9073-9_17

Cited by 3 publications

(3 citation statements)

References 108 publications

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“…Transcription of the HN unit and the F unit is responsible for the constitution of the HN (hemagglutinin-neuraminidase) and F (fusion) protein, respectively. On the envelope of the virus, both of these proteins act as virus neutralizing and protective antigens of NDV ( 1 , 21 , 22 ). The F protein of NDV is basically a type I membrane glycoprotein.…”

Section: Introductionmentioning

confidence: 99%

Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn

et al. 2020

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Newcastle disease (ND) is a viral disease that causes labored breathing, periorbital oedema, and ataxia in the majority of avian species. The available vaccines against Newcastle disease virus (NDV) are limited, owing to their low reactivity and multiple dosage requirements. Plant-based machinery provides an attractive and safe system for vaccine production. In the current study, we attempted to express fusion (F) and hemagglutinin-neuraminidase (HN) proteins (the protective antigens against NDV) under constitutive 35S and seed-specific Zein promoters, respectively. Almost 2-7.1-fold higher expression of F gene mRNA in transgenic corn leaves and 8-28-fold higher expression of HN gene mRNA in transgenic corn seeds were observed, when the expression was analyzed by real-time PCR on a relative basis as compared to non-transgenic control plant material (Leaves and seeds). Similarly, 1.66 µg/ml of F protein in corn leaves, i.e., 0.5% of total soluble protein, and 2.4 µg/ml of HN protein in corn seed, i.e., 0.8% of total seed protein, were found when calculated through ELISA. Similar levels of immunological response were generated in chicks immunized through injection of E. coli-produced pET F and pET HN protein as in chickens orally fed leaves and seeds of maize with expressed immunogenic protein. Moreover, the detection of anti-NDV antibodies in the sera of chickens that were fed maize with immunogenic protein, and the absence of these antibodies in chickens fed a normal diet, confirmed the specificity of the antibodies generated through feeding, and demonstrated the potential of utilizing plants for producing more vaccine doses, vaccine generation at higher levels and against other infectious diseases.

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Section: Introductionmentioning

confidence: 99%

Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn

et al. 2020

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“…the two binding sites of the hN globular head (sites 1 and 2) are responsible for its neuraminidase and fusion activities, respectively. the stalk region promotes fusion by interacting with the f protein, which is responsible for virion penetration into the cell surface (Ewies et al, 2017;Jin et al, 2017;dey et al, 2019). the hN protein possesses neuraminidase activity; it binds sialic-acid-containing receptors (hemagglutination) on host cells.…”

Section: Introductionmentioning

confidence: 99%

E. coli expression and immunological assessment of expressed recombinant Newcastle disease virus hemagglutinin-neuraminidase protein in chickens

Shahid¹,

Rao²,

Ahad³

et al. 2020

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Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NdV). developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (hN) protein can protect the poultry industry. in the present study, the full-length hN protein was expressed in Escherichia coli (E. coli) Bl21 (dE3) cells, purified via affinity chromatography and detected via western blot analysis using his-specific antibodies. the purified hN protein was further evaluated in chickens to study the immune response against NdV. the successful production of hN-specific igY proved the activity of the purified hN protein. igY was present in the serum of immunized chickens. however, the immune response was higher in chickens immunized with purified hN protein along with complete and incomplete adjuvants than in chickens immunized with only the hN protein.

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“…For a long time, IBD has been reported to be one of the major hindrance factor for the poultry farming globally (Dey et al, 2019;Sainsbury, 2000). However, the disease has become more devastating especially in the last two decades after the appearance of very virulent and variant strains (Mutinda, 2016).…”

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confidence: 99%

Factors associated with infectious bursal disease vaccination failure in chickens in Tanzania

Saidi¹

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Infectious bursal disease (IBD) is a viral infection that affects young chicks. The IBD outbreaks in vaccinated chickens are frequently reported in Tanzania. The current study was conducted to determine the possible causes of vaccination failure focusing on knowledge, attitude and practices (KAPs) of vaccine sellers and poultry farmers, maternally derived antibodies (MDA), immunogenic potential of Virgo 7 vaccine and the phylogenetic relationship between the vaccine and the field strains. A cross-sectional study was performed to gather information on KAPs from 384 farmers and 20 veterinary outlets in Dar es Salaam. Results revealed inadequate knowledge of farmers in IBD management and breaches in the cold chain maintenance by vaccine sellers. A total of 60 chicks were experimentally vaccinated with Virgo 7 strain vaccine and titers of induced antibodies assessed. The vaccine induced adequate antibodies against IBDV, this confirming its immunogenic efficacy. Isolated nucleic acids from the vaccine and field strains were sequenced and result shows that field isolates are genetically different from the vaccine strains used in the country. The field isolates belong to the vvIBDV African types, while the vaccines belong to the vvIBDV European or classical virulent types. Putting together results from this study reveals multiple possible reasons which may contribute to vaccine failures. These include poor vaccine handling by farmers and vaccine seller and the genetic disparity between the field and vaccine strains. It is therefore recommended that veterinary regulatory authorities should ensure good vaccine handling practices and considering local virus isolates during vaccine development.

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Newcastle Disease Virus

Cited by 3 publications

References 108 publications

Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn

Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn

E. coli expression and immunological assessment of expressed recombinant Newcastle disease virus hemagglutinin-neuraminidase protein in chickens

Factors associated with infectious bursal disease vaccination failure in chickens in Tanzania

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