Niastella hibisci sp. nov., isolated from rhizosphere soil of mugunghwa, the Korean national flower

Abstract

A Gram-stain-negative, aerobic, non-motile, long rods with no flagellum strain, designated THG-YS3.2.1T, was isolated from rhizosphere soil of mugunghwa, collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40 °C (optimum 28 °C), at pH 6.0-8.0 (optimum pH 7.0) and with 0-1.0 % NaCl (optimum 1.0 %). The predominant menaquinone was menaquinone-7 (MK-7). The major cellular fatty acids were anteiso-C12 : 0, iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1 G, anteiso-C15 : 1 A, C15 : 0 2-OH, C16 : 0, iso-C16 : 0, C16 : 0 3-OH, iso-C16 : 0 3-OH, iso-C16 : 1 G, C17 : 0 2-OH, iso-C17 : 0 3-OH, C17 : 1ω6c, C18 : 3ω6c (6, 9, 12), C18 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major polar lipids were phosphatidylmethylethanolamine), phosphatidylethanolamine, five unidentified aminolipids and three unidentified lipids. The DNA G+C content of strain THG-YS3.2.1T was 45.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.1T were Niastella populi KCTC 22560T (98.7 % 16S rRNA gene sequence similarity), Niastella gongjuensis KACC 17399T (96.9 %), Niastella vici KCTC 42474T (96.2 %), Niastella yeongjuensis KACC 11466T (95.5 %) and Niastella koreensis KACC 11465T (95.1 %). DNA-DNA hybridization values between strain THG-YS3.2.1T and N. populi KCTC 22560T, N. gongjuensis KACC 17399T, N.vici KCTC 42474T, N. yeongjuensis KACC 11466T and N. koreensis KACC 11465Twere 55.8±1.0, 39.5±0.5, 35.2±0.1, 17.6±0.3 and 12.5±1.2 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.1T represents a novel species of the genus Niastella, for which the name Niastellahibisci sp. nov. is proposed. The type strain is THG-YS3.2.1T (=KCTC 52084T=CCTCC AB 2015356T).

Niastella caeni sp. nov., isolated from activated sludge

“…Besides, 6062 CDSs and 56 RNAs were predicted. The DNA G+C content of strain HX-16-21 T acquired from the genome sequence was 43.5 mol%, a value within the range reported for strains belonging to the genus Niastella [1][2][3][4][5]. Additionally, among the 5 genomes of members in genus Niastella, strain HX-16-21 T possessed the smallest genome size.…”

“…S2). The only detected respiratory quinone was menaquinone MK-7 (MK-7), which generally also existed in other members of the genus Niastella [1][2][3][4][5].…”

“…The genus Niastella, proposed by Weon et al in 2006 [1] and emended by Zhang et al in 2010 [2], belongs to the family Chitinophagaceae, phylum Bacteroidetes. Up to now, this genus comprises six species: Niastella koreensis [1], Niastella yeongjuensis [1], Niastella populi [2], Niastella gongjuensis [3], Niastella vici [4] and Niastella hibisci [5]. All of the Niastella species with validly published names are Gram-stain-negative, aerobic and filamentous rods.…”

“…Chemotaxonomically, members of the genus are characterized by menaquinone 7 (MK-7) as the predominant respiratory ubiquinone and iso-C 15 : 0 , iso-C 15 : 1 G and iso-C 17 : 0 3-OH as the main fatty acid components. The DNA G+C content ranges from 41.4 to 45.8 mol% [1][2][3][4][5]. In this study, we characterize strain HX-16-21 T , which was isolated from an activated sludge sample collected from an agricultural chemical plant.…”

“…Differential characteristics of strain HX-16-21 T and related type strains of the genus Niastella Strains: 1, HX-16-21 T (data from this study); 2, Niastella vici CCTCC AB 2015052 T (data from this study); 3, Niastella koreensis KACC 11465 T [1]; 4, Niastella yeongjuensis KACC 11466 T [1]; 5, Niastella populi KCTC 22560 T [2]; 6, Niastella gongjuensis KACC 17399 T [3]; 7, Niastella hibisci KCTC 52084 T[5]. All strains are Gram-stain-negative, non-flagellated and aerobic.…”