Niche-Specific Adaptive Evolution of Lactobacillus plantarum Strains Isolated From Human Feces and Paocai

Pan, Qiqi; Cen, Shi; Yu, Leilei; Tian, Fengwei; Zhang, Hao; Zhai, Qixiao

doi:10.3389/fcimb.2020.615876

Cited by 13 publications

(9 citation statements)

References 60 publications

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“…Furthermore, although we focused on the metabolism of adipose tissue as a possible mechanism of the control of obesity and diabetes, liver has an even greater role than adipose tissue in energy metabolism, such that more comprehensive studies including assessments using metabolic cages are needed. Finally, even when they belong to the same species, different microbial strains differ genetically and perhaps functionally [92][93][94] . Our findings were obtained by using a type strain, and the actual strains involved in the clinical effect should be isolated and subjected to single-cell analysis and other evaluation.…”

Section: Discussionmentioning

confidence: 99%

Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota

et al. 2022

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The gut microbiome is an important determinant in various diseases. Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus. Oral administration of B. wexlerae to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet–induced obesity and diabetes. The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and l-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition. These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.

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Section: Discussionmentioning

confidence: 99%

Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota

et al. 2022

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“…L. plantarum has been classified as a “nomadic” or “generalist” bacterium because of its diverse habitats ( Martino et al, 2016 ; Choi et al, 2018 ; Yu et al, 2020 , 2021 ). Consistent with its broad environmental and host ranges, L. plantarum strains have significant intraspecific genetic and phenotypic versatility due to strain-specific genes ( Molenaar et al, 2005 ; Zheng et al, 2015 ; Duar et al, 2017 ; Salvetti et al, 2018 ; Cen et al, 2020 ; Pan et al, 2021 ). The latest taxonomic research has elevated L. plantarum subsp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of IR Biotyper for Lactiplantibacillus plantarum Typing and Its Application Potential in Probiotic Preliminary Screening

Zhu

Wang

et al. 2022

Front. Microbiol.

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IR Biotyper (IRBT), which is a spectroscopic system for microorganism typing based on Fourier transform infrared (FTIR) technology, has been used to detect the spread of clones in clinical microbiology laboratories. However, the use of IRBT to detect probiotics has rarely been reported. Herein, we evaluated the discriminatory power of IRBT to type Lactiplantibacillus plantarum isolates at the strain level and explored its application potential in probiotic preliminary selection. Twenty Lactiplantibacillus isolates collected from pickled radishes during successive fermentation were used to test the robustness of IRBT at the strain level. IRBT was then compared with genotyping methods such as whole-genome sequencing (WGS), pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) to evaluate its discrimination power. IRBT distributed the 20 isolates into five clusters, with L. argentoratensis isolate C7-83 being the most distant from the other isolates, which belonged to L. plantarum. IRBT showed good reproducibility, although deviation in the discriminative power of IRBT was found at the strain level across laboratories, probably due to technical variance. All examined methods allowed bacterial identification at the strain level, but IRBT had higher discriminatory power than MLST and was comparable to the WGS and PFGE. In the phenotypic comparison study, we observed that the clustering results of probiotic physiological attributes (e.g., sensitivity to acid and bile salts, hydrophobicity of the cell surface, and resistance to antibiotics) were consistent with the typing results of IRBT. Our results indicated that IRBT is a robust tool for L. plantarum strain typing that could improve the efficiency of probiotic identification and preliminary screening, and can potentially be applied in probiotic traceability and quality control.

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“…9 Additionally, L. plantarum, among other Lactobacilli species, exhibit nichespecific genetic alterations that have been linked to this organism's ability to alter its metabolism and regulate the composition of the surrounding microbial community. [29][30][31] This study characterized the ability of L. plantarum to utilize biomolecules commonly released by microbes to support EET via overlapping, but unique mechanisms. We assert that multiple EET routes have been maintained by L. plantarum to support survival in diverse environments and which route is preferred is dependent on the availability of redox active molecules in the local environment and the cellular status (i.e.…”

Section: Discussionmentioning

confidence: 99%

The differing roles of flavins and quinones in extracellular electron transfer in Lactiplantibacillus plantarum

Tolar

Ajo‐Franklin

2022

Preprint

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Lactiplantibacillus plantarum is a homofermentative lactic acid bacteria that is commonly found in the human gut and fermented food products. Despite its overwhelmingly fermentative metabolism, this microbe can perform extracellular electron transfer (EET) when provided with an exogenous quinone, 1,4-dihydroxy-2-naphthoic acid (DHNA) and riboflavin. However, the separate roles of DHNA and riboflavin in EET in L. plantarum has remained unclear. Here we seek to understand the role of quinones and flavins for EET by monitoring iron and anode reduction in the presence and absence of these small molecules. We found that either addition of DHNA or riboflavin can support robust iron reduction, indicating electron transfer to extracellular iron occurs through both flavin-dependent and DHNA-dependent routes. Using genetic mutants of L. plantarum, we found that flavin-dependent iron reduction requires Ndh2 and EetA, while DHNA-dependent iron reduction largely relies on Ndh2 and PplA. In contrast to iron reduction, DHNA-containing media supported more robust anode reduction than riboflavin-containing media, suggesting electron transfer to an anode proceeds most efficiently through the DHNA-dependent pathway. Furthermore, we found that flavin-dependent anode reduction requires EetA, Ndh2, and PplA, while DHNA-dependent anode reduction requires Ndh2 and PplA. Taken together, we identify multiple EET routes utilized by L. plantarum and show that the EET route depends on access to environmental biomolecules and on the extracellular electron acceptor. This work expands our molecular-level understanding of EET in Gram-positive microbes and provides additional opportunities to manipulate EET for biotechnology.

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Niche-Specific Adaptive Evolution of Lactobacillus plantarum Strains Isolated From Human Feces and Paocai

Cited by 13 publications

References 60 publications

Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota

Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota

Evaluation of IR Biotyper for Lactiplantibacillus plantarum Typing and Its Application Potential in Probiotic Preliminary Screening

The differing roles of flavins and quinones in extracellular electron transfer in Lactiplantibacillus plantarum

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