Nicotine Inhibits Mineralization of Human Dental Pulp Cells

Smoking Modulates Gene Expression of Type I Collagen, Bone Sialoprotein, and Osteocalcin in Human Alveolar Bone

“…They found that a low nicotine concentration increases osteoblast-like cell proliferation and the gene expression that mediates bone metabolism. Similarly to the present study, COL-I expression was increased in osteosarcoma cells 37 and in human dental pulp cells 38 in the presence of nicotine, especially in low doses. 37 COL-I, a fibril-forming collagen, is found in most connective tissues and is abundant in bone.…”

“…Nicotine stimulated the deposition of Ca 2+ in ROB-C26 cells, which are characteristic of the early stage of cell differentiation, and decreased the deposition of Ca 2+ in MC3T3-E1 cells, which are characteristic of the late stage of differentiation of osteoblasts. 38,41 The same time-dependent effect of tobacco on healing was observed in the study by Yamano et al 17 They observed an increased expression of BMP-2, BSP, and type II collagen mRNA in bone harvested from around titanium implants installed in the tibias of rats after 2 weeks. After 4 weeks, in a ''mature'' bone healing area, genes, including BMP-2, BSP, OPN, type II collagen, and core-binding factor subunit a-1, were less expressed.…”

“…[47][48][49] In contrast, OCN is a calcium-binding protein of bone and the most abundant noncollagenous protein of mineralized tissue. 50 It is not present in the first crystal formation but in fully mineralized matrix, 38 indicating its role in the mineralization and maturation of bone tissue. In this context, once BSP is inhibited by smokewith a consequent decrease in crystal nucleates and the collagenous matrix-OCN could be less expressed and lead to a less mineralized tissue.…”

Fibroblast Growth Factor–2 Regulates the Cell Function of Human Dental Pulp Cells

“…All 3 primary cell isolations were examined for the several extracellular matrix proteins by using reverse transcription-polymerase chain reaction. We confirmed that the cells expressed mRNA of type I collagen, dentin matrix protein-1 (DMP-1), and dentin sialoprotein; the latter 2 proteins are known to be specific to dentin (23). All primary cell isolations provided similar results in each experiment of this study.…”

Hypoxia Promotes Mineralization of Human Dental Pulp Cells

“…When cell growth had reached confluence, the cells were detached by trypsinization (0.2% trypsin and 0.02% ethylenediaminetetraacetic acid; Gibco) and subcultured with Dulbecco modified Eagle medium plus 10% fetal bovine serum. In some cases, the culture medium was supplemented with 10 mmol/L b-glycerolphosphate (Sigma-Aldrich, St Louis, MO), 50 mg/mL ascorbic acid, and 10 À8 mol/L dexamethasone to induce mineralization (16).…”