Nippostrongylus brasiliensis Histochemical changes in the composition of mucins in goblet cells during infection in rats

Koninkx, J. F. J. G.; Mirck, M. H.; Hendriks, Henno; Mouwen, J. M. V. M.; Dijk, J. E. van

doi:10.1016/0014-4894(88)90109-9

Cited by 52 publications

(30 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, eosinophils known for releasing potent cytotoxic and proinflammatory mediators have been demonstrated to protect against repeated exposure to GI parasites, such as T. spiralis. Goblet cell hyperplasia is associated with worm expulsion of Strongyloides ratti (7), Trichuris muris (11), and T. spiralis (18,26), with N. brasiliensis (32,44,45) being the most efficient example, as it is vulnerable to both the goblet cell hyperplastic response and a qualitative change in mucin glycoproteins (34). In our study, mice infected with S. obvelata elicited no significant eosinophilia, mastocytosis, and/or goblet cell hyperplasia to suggest that any of these responses contributed to the expulsion of the nematode.…”

Section: Discussionmentioning

confidence: 99%

Infection withSyphacia obvelata(Pinworm) Induces Protective Th2 Immune Responses and Influences Ovalbumin-Induced Allergic Reactions

et al. 2006

View full text Add to dashboard Cite

Infections with pinworms are common in rodent animal facilities. In this study, we show the consequence of an outbreak in a transgenic barrier facility of infection by Syphacia obvelata, a murine pinworm gastrointestinal nematode. Immune responses were defined in experimental infection studies with BALB/c mice. Infection with S. obvelata induced a transient Th2-type immune response with elevated interleukin 4 (IL-4), IL-5, and IL-13 cytokine production and parasite-specific immunoglobulin G1 (IgG1). In contrast, BALB/c mice deficient in IL-13, IL-4/13, or the IL-4 receptor alpha chain showed chronic disease, with a >100-fold higher parasite burden, increased gamma interferon production, parasite-specific IgG2b, and a default Th2 response. Interestingly, infected IL-4 ؊/؊ BALB/c mice showed only slightly elevated parasite burdens compared to the control mice, suggesting that IL-13 plays the dominant role in the control of S. obvelata. The influence that pinworm infection has on the allergic response to a dietary antigen was found to be important. Helminth-infected mice immunized against ovalbumin (Ova) elicited more severe anaphylactic shock with reduced Ova-specific IL-4 and IL-5 than did noninfected controls, demonstrating that S. obvelata infection is able to influence nonrelated laboratory experiments. The latter outcome highlights the importance of maintaining mice for use as experimental models under pinworm-free conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Infection withSyphacia obvelata(Pinworm) Induces Protective Th2 Immune Responses and Influences Ovalbumin-Induced Allergic Reactions

et al. 2006

View full text Add to dashboard Cite

show abstract

“…In Nyppostrongilus brasiliensis-infected rats, Konink et al (1988) demonstrated a switch from neutral to acidic mucins, with sulphomucins predominating within acid glycoconjugates. Usenov (1981) showed that during larval migration the amount of acid mucopolysaccharides increased in lambs infected with Nematodirus sp.…”

Section: Discussionmentioning

confidence: 99%

“…(Usenov 1981), in intestinal villi during the immune cell-mediated expulsion of Nyppostrongilus brasiliensis from the rat intestine (Miller & Nawa 1979, Miller et al 1981, Miller & Huntley 1982, Miller 1987, in the stomach and small intestine of rats infected with Taenia taeniaeformis (Cook & Williams 1981), and in mice small intestine parasitized with Hymenolepis diminuta (McKay et al 1990) and Trichinella spiralis (Kahn et al 2001). Qualitative changes in goblet cell secretions have also been described in response to the presence of parasites (Konink et al 1988, Ishikawa et al 1993, Fairweather 1997, Manjili et al 1998, and it has been suggested that an increased secretion of chemically different mucus contributes to the elimination of parasites from the alimentary tract (Ishikawa et al 1993, Miller 1987.…”

Section: Introductionmentioning

confidence: 99%

Histochemistry of glycoconjugates in mucous cells of Salmo trutta uninfected and naturally parasitized with intestinal helminths

Bosi¹,

Arrighi²,

Giancamillo³

et al. 2005

Dis. Aquat. Org.

View full text Add to dashboard Cite

Mucus secreted onto the surface of the intestine forms a physical barrier to invading parasites so that a possible attachment of helminths to the surface is prevented and their expulsion by peristalsis facilitated. In mammals, intestinal parasites induce hyperplasia and hypertrophy of intestine goblet cells and provoke changes in the mucus composition. In fish, this topic has received less attention. In the present investigation, histochemical methods were employed to compose intestinal mucous cell numbers and their glycoconjugate composition were compared by uninfected brown trout Salmo trutta and in S. trutta parasitized with Cyathocephalus truncatus or Pomphorhynchus laevis. When P. laevis was present in the intestine of the brown trout, the total mucous cell number, and the number of mucous cells containing acid or mixed glycoconjugates were significantly enhanced. No significant change in the total mucous cell number was detected in the intestine of fish parasitized with C. truncatus in comparison with uninfected brown trout. A significant increase was observed in the number of both acid (especially sulphated) and mixed glycoconjugates containing mucous cells as well as a significant decrease in the number of neutral glycoconjugates containing mucous cells. When intestinal helminths were present, the thickness of the adherent mucous gel increased. In a limited number of other fish species, the occurrence of gill and intestinal parasites has been reported to increase the mucosal glycoconjugate secretions. Our study is the first quantitative report on the effects of intestinal helminths on the density of mucous cells and mucus composition in a fish species. KEY WORDS: Glycoconjugates · Mucus composition · Mucous cell number · Intestinal parasites · Brown troutResale or republication not permitted without written consent of the publisher

show abstract

“…Before or around the time of worm rejection, a variety of pathological changes occur in the intestinal mucosa; these changes include edema, eosinophil infiltration, mastocytosis, and partial villus atrophy with crypt hyperplasia (9,13,25). However, the alterations resolve quickly after worm rejection, along with the occurrence of goblet cell hyperplasia with mucins that have altered sugar residues (15,19,21). Nawa et al (21) demonstrated that mucins are highly selective and specific effectors for N. brasiliensis worm expulsion, and McKenzie et al (18) reported a possible link between the Th2 cytokine interleukin-13 and the production of intestinal mucus.…”

mentioning

confidence: 99%

Up-Regulation of Fas (CD95) and Induction of Apoptosis in Intestinal Epithelial Cells by Nematode - Derived Molecules

et al. 2002

View full text Add to dashboard Cite

Infection by the intestinal nematode Nippostrongylus brasiliensis induces acceleration of apoptosis in the small intestinal villus epithelial cells in vivo. In the present study, we examined whether worm extract or excretory-secretory product induces apoptosis in the rat intestinal epithelial cell line IEC-6 in vitro. In the presence of worm extract or excretory-secretory product (>6 g/ml), IEC-6 cell growth was significantly suppressed, and there was a concomitant increase in the number of detached cells in culture dishes. Detached cells showed nuclear fragmentation, activation of caspase-3, and specific cleavage of poly(ADP-ribose) polymerase, suggesting that apoptosis was induced in these cells. Semiquantitative reverse transcription-PCR showed that expression of Fas (CD95) mRNA was up-regulated as early as 6 h after addition of excretorysecretory product, while Fas ligand expression and p53 expression were not up-regulated. Fluorescenceactivated cell sorter analyses revealed a significant increase in Fas expression and a slight increase in FasL expression in IEC-6 cells cultured in the presence of excretory-secretory product, while control IEC-6 cells expressed neither Fas or FasL. These results indicated that N. brasiliensis worms produce and secrete biologically active molecules that trigger apoptosis in intestinal epithelial cells together with up-regulation of Fas expression, although the mechanism of induction of apoptosis remains to be elucidated.

show abstract

Nippostrongylus brasiliensis Histochemical changes in the composition of mucins in goblet cells during infection in rats

Cited by 52 publications

References 17 publications

Infection withSyphacia obvelata(Pinworm) Induces Protective Th2 Immune Responses and Influences Ovalbumin-Induced Allergic Reactions

Infection withSyphacia obvelata(Pinworm) Induces Protective Th2 Immune Responses and Influences Ovalbumin-Induced Allergic Reactions

Histochemistry of glycoconjugates in mucous cells of Salmo trutta uninfected and naturally parasitized with intestinal helminths

Up-Regulation of Fas (CD95) and Induction of Apoptosis in Intestinal Epithelial Cells by Nematode - Derived Molecules

Contact Info

Product

Resources

About