Nitrate and nitrite injection during municipal solid waste anaerobic biodegradation

Vigneron, Vassilia; Ponthieu, Marie; Barina, Giulia; Audic, J. M.; Duquennoi, C.; Mazéas, Laurent; Bernet, Nicolas; Bouchez, Théodore

doi:10.1016/j.wasman.2006.02.020

Cited by 51 publications

(30 citation statements)

References 23 publications

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“…DNRA is known to occur in very reduced environments with either sulfide or high chemical oxygen demand to NO 3 -ratios (Tugtas and Pavlostathis 2007b;Akunna et al 1992). Injection of nitrate into an anaerobic digester treating municipal solid waste was associated with ammonium accumulation and DNRA was suspected (Vigneron et al 2007). In this study the most noteworthy distinguishing feature between cultures suspected of carrying out DNRA compared to denitrification, is that the latter was less inhibited by NO x -compounds.…”

Section: Discussionmentioning

confidence: 66%

Nitrate and nitrite inhibition of methanogenesis during denitrification in granular biofilms and digested domestic sludges

2009

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Anaerobic bioreactors that can support simultaneous microbial processes of denitrification and methanogenesis are of interest to nutrient nitrogen removal. However, an important concern is the potential toxicity of nitrate (NO(3) (-)) and nitrite (NO(2) (-)) to methanogenesis. The methanogenic toxicity of the NO (x) (-) compounds to anaerobic granular biofilms and municipal anaerobic digested sludge with two types of substrates, acetate and hydrogen, was studied. The inhibition was the severest when the NO (x) (-) compounds were still present in the media (exposure period). During this period, 95% or greater inhibition of methanogenesis was evident at the lowest concentrations of added NO(2) (-) tested (7.6-10.2 mg NO(2) (-)-N l(-1)) or 8.3-121 mg NO(3) (-)-N l(-1) of added NO(3) (-), depending on substrate and inoculum source. The inhibition imparted by NO(3) (-) was not due directly to NO(3) (-) itself, but instead due to reduced intermediates (e.g., NO(2) (-)) formed during the denitrification process. The toxicity of NO (x) (-) was found to be reversible after the exposure period. The recovery of activity was nearly complete at low added NO (x) (-) concentrations; whereas the recovery was only partial at high added NO (x) (-) concentrations. The recovery is attributed to the metabolism of the NO (x) (-) compounds. The assay substrate had a large impact on the rate of NO(2) (-) metabolism. Hydrogen reduced NO(2) (-) slowly such that NO(2) (-) accumulated more and as a result, the toxicity was greater compared to acetate as a substrate. The final methane yield was inversely proportional to the amount of NO (x) (-) compounds added indicating that they were the preferred electron acceptors compared to methanogenesis.

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Section: Discussionmentioning

confidence: 66%

Nitrate and nitrite inhibition of methanogenesis during denitrification in granular biofilms and digested domestic sludges

2009

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“…The measurement conditions were same as in Vigneron et al (2007). To analyze stable carbon isotopic signature of CH 4 (d 13 C-CH 4 ) and CO 2 (d 13 C-CO 2 ) gas samples were collected by a syringe, transferred into 5 ml vacuumed serum bottles, and stored for later isotopic analysis performed using a Trace GC Ultra (Thermo Electron Corporation, USA) attached to a Delta V plus isotope ratio mass spectrometer (Thermo Electron Corporation, USA) via a Finnigan GC combustion III with an unique combination of microcombustion oxidation furnace maintained at 940°C.…”

Section: Methodsmentioning

confidence: 99%

Methanosarcina as the dominant aceticlastic methanogens during mesophilic anaerobic digestion of putrescible waste

Вавилин

Mazéas³

et al. 2008

Antonie van Leeuwenhoek

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Taking into account isotope (13)C value a mathematical model was developed to describe the dynamics of methanogenic population during mesophilic anaerobic digestion of putrescible solid waste and waste imitating Chinese municipal solid waste. Three groups of methanogens were considered in the model including unified hydrogenotrophic methanogens and two aceticlastic methanogens Methanosaeta sp. and Methanosarcina sp. It was assumed that Methanosaeta sp. and Methanosarcina sp. are inhibited by high volatile fatty acids concentration. The total organic and inorganic carbon concentrations, methane production, methane and carbon dioxide partial pressures as well as the isotope (13)C incorporation in PSW and CMSW were used for the model calibration and validation. The model showed that in spite of the high initial biomass concentration of Methanosaeta sp. Methanosarcina sp. became the dominant aceticlastic methanogens in the system. This prediction was confirmed by FISH. It is concluded that Methanosarcina sp. forming multicellular aggregates may resist to inhibition by volatile fatty acids (VFAs) because a slow diffusion rate of the acids limits the VFA concentrations inside the Methanosarcina sp. aggregates.

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“…Denitrification was the main process responsible for nitrogen removal under anaerobic conditions. It was also found in recent years that anaerobic ammonium oxidation (ANAMMOX) and dissimilatory nitrate reduction to ammonium (DNRA) took place under anaerobic or anoxic conditions (Ahn, 2006;Vigneron et al, 2007). N in gas production accounted for 93.56% of TON-N added to the denitrification column, which suggested denitrification might be the dominant reaction responsible for TON removal in the denitrification column.…”

Section: In Situ Denitrification and Methanogenesismentioning

confidence: 97%

Nitrogen removal from landfill leachate via ex situ nitrification and sequential in situ denitrification

Zhong

Tao

et al. 2009

Waste Management

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Nitrate and nitrite injection during municipal solid waste anaerobic biodegradation

Cited by 51 publications

References 23 publications

Nitrate and nitrite inhibition of methanogenesis during denitrification in granular biofilms and digested domestic sludges

Nitrate and nitrite inhibition of methanogenesis during denitrification in granular biofilms and digested domestic sludges

Methanosarcina as the dominant aceticlastic methanogens during mesophilic anaerobic digestion of putrescible waste

Nitrogen removal from landfill leachate via ex situ nitrification and sequential in situ denitrification

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