Nitric Oxide Biosensors Based on Hb/Phosphatidylcholine Films

Fan, Chunhai; Pang, Jiantao; Shen, Pingping; Li, Genxi; Zhu, Dunru

doi:10.2116/analsci.18.129

Cited by 38 publications

(24 citation statements)

References 19 publications

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“…Km app in this Hb-silver NPs system is estimated to be 100.80 µM, which is much smaller compared with the previous reports, 24 indicating a high affinity of Hb and NO. In order to test the applicability of this sensor, its selectivity is evaluated by NO determinations in the presence of some potentially co-existing compounds of NO in biological systems.…”

Section: Resultscontrasting

confidence: 75%

“…According to our previous studies, this new peak is ascribed to the reduction of NO induced by Hb. 22,24 Therefore, silver NPs not only give Hb an appropriate folding configuration, preserving the natural conformation of Hb, but also facilitate its catalytic reactivity and sensitivity towards the reduction of NO.…”

Section: Resultsmentioning

confidence: 99%

“…NO has been observed to react with hemoglobin (Hb) specially and its reduction can be catalyzed by the protein, which has educed a new hope of designing NO biosensors based on Hb modified electrodes. [19][20][21][22][23][24] On the other hand, recently, nanoparticles (NPs) have attracted great attention because of their excellent performances in a variety of areas including sensor devices, [25][26][27][28][29][30][31][32] since they have quanta character of small granule diameter with large specific surface area and a relatively strong reactivity. NPs have been effectively employed to obtain the direct electrochemistry of Hb, 33,34 as well as to develop NO biosensors.…”

Section: Introductionmentioning

confidence: 99%

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An Electrochemical Biosensor for Nitric Oxide Based on Silver Nanoparticles and Hemoglobin

et al. 2004

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A nitric oxide (NO) biosensor based on silver nanoparticles was fabricated with high sensitivity and selectivity as well as stability. Silver nanoparticles could preserve the microstructures of hemoglobin, but the electrochemical reactivity of the protein and its detection sensitivity toward NO could be greatly enhanced. Accordingly, a NO biosensor was developed. The linear concentration range was from 1.0 × 10 -6 to 5.0 × 10 -5 M. Its detection limit was 3.0 × 10 -7 M with a sensitivity of 0.0424 µA µM -1 NO. The possible co-existing compounds would not interfere with the detection.

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Section: Resultscontrasting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Electrochemical Biosensor for Nitric Oxide Based on Silver Nanoparticles and Hemoglobin

et al. 2004

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“…[9][10][11][12] Specifically, others and ourselves have developed a heme protein based approach for NO detection, which takes advantage of the high affinity of NO towards heme proteins. [13][14][15][16] Kieselguhr is a kind of inorganic material with porous structure, which some small molecules such as H 2 O and H 3 O + , can pass through. Due to its good mechanical, thermal and chemical stability, kieselguhr promises to be an immobilization matrix.…”

Section: Introductionmentioning

confidence: 99%

A Nitric Oxide Biosensor Based on Horseradish Peroxidase/Kieselguhr Co‐Modified Pyrolytic Graphite Electrode

et al. 2004

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A reagentless nitric oxide (NO) biosensor was prepared using a pyrolytic graphite (PG) electrode modified with a composite film containing horseradish peroxidase (HRP) and kieselguhr. Noticeably, the electron-transfer reactivity of HRP was significantly enhanced when incorporated in the inorganic kieselguhr material. Consequently, we observed the direct electrochemical response of HRP in this composite film, which would be otherwise electrochemically silent (in the absence of kieselguhr). Importantly, this modified electrode demonstrated nice catalytic activity, as well as high stability, towards the reduction of NO. The peak current related to NO was linearly proportional to its concentration from 2.0 x 10(-7) to 2.0 x 10(-5) mol/L, and the relative standard deviation was 4 % for five successive determinations at a NO concentration of 1.0 x 10(-5) mol/L. The critical level in concentration was estimated to be (4.0 +/- 0.3) x 10(-8) mol/L.

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“…* * Electrochemical Society Active Member. z E-mail: cbparker@duke.edu films, 22 hemoglobin-DNA film, 23 and ionic polymers and alphacyclodextrin. 24 In novel electrochemical systems employing platinumbased systems, [25][26][27] myoglobin-clay, 28 and indium hexacyanoferrate film-modified electrodes 29 have also been described.…”

mentioning

confidence: 99%

Diamond for Biosensing: Electrochemical Detection of NOx Species with Thiol-Amine Functionalized Diamond

Sund

Wolter

Natishan

et al. 2015

J. Electrochem. Soc.

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Diamond has long been of interest as a biomaterial due to its expected biocompatibility and chemical stability. Progress in surface functionalization of diamond and diamond electrochemistry has extended this interest to use as a biosensor platform. The sensitivity and selectivity of the diamond surface can be enhanced by covalently attaching carbon-based biochemicals. In this communication, an amine-thiol molecule was attached to a boron-doped diamond (BDD) surface to produce an enhanced ability to detect NO species. In the case of hydrogen terminated BDD, it was found that NO (aqueous) oxidation was insufficiently distinct to electrochemically detect NO. However, the amine-thiol functionalized BDD surface provided a distinct oxidation peak from 65 nanomolar to 236 nanomolar concentrations. Multiple cyclic voltammagrams with amine-thiol functionalized BDD electrode in (HPLC-quality) purified water indicated that the covalently attached surface molecules resisted decomposition during both anodic and cathodic polarization. The detection and monitoring of nitric oxide (i.e., nitrogen monoxide (NO)) has become of increasing interest. NO can arise as a pollutant from the combustion of air in automobiles and power plants, is an intermediate for nitric acid in semiconductor processes, and is a diverse signaling molecule in the human body. It plays important roles in vasodilation, anti-infection, and neurotransmission.1 The ability to detect NO in aqueous environments with robust and sensitive sensors would provide a means to monitor levels of dissolved NO emissions and to trace transient concentrations of NO in biomedical applications. In this work we take a biomimetic approach for functionalizing the BDD surface. In the human body, the amino acid cysteine is comprised of a thiol moiety (RSH) and carries NO in the form of RSNO. Thus, one can envision using a thiol functionalized surface to engineer a sensor for the detection of NO. This is particularly important because current NO sensors are limited by the need for a highly selective membrane.NO detection.-Because nitric oxide has a short half-life, NO sensors should be selective, responsive, and capable of being miniaturized to support continuous monitoring of in-vivo processes in biomedical applications. About 10% of NO-related studies involve direct measurements.2 In a review of nitric oxide selective electrodes, Davies and Zhang 2 indicated that electrochemical detection systems, especially amperometric, provide the best sensitivity and simple supporting apparatus compared to alternative techniques such as chemiluminescence, 3 the Griess assay, 4 paramagnetic resonance spectrometry, 5,6 and various bioassays. 7 Of these sensing approaches, electrochemical techniques are the most viable for a robust sensor, as they provide selectivity, durability, and portability.Davies and Zhang have previously noted that in addition to electrochemical detection of NO with simple organic and inorganic interfaces, more complex interfaces have been used, incorporating chelating mole...

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Nitric Oxide Biosensors Based on Hb/Phosphatidylcholine Films

Cited by 38 publications

References 19 publications

An Electrochemical Biosensor for Nitric Oxide Based on Silver Nanoparticles and Hemoglobin

An Electrochemical Biosensor for Nitric Oxide Based on Silver Nanoparticles and Hemoglobin

A Nitric Oxide Biosensor Based on Horseradish Peroxidase/Kieselguhr Co‐Modified Pyrolytic Graphite Electrode

Diamond for Biosensing: Electrochemical Detection of NOx Species with Thiol-Amine Functionalized Diamond

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