Nitric oxide-mediated cytosolic glucose-6-phosphate dehydrogenase is involved in aluminum toxicity of soybean under high aluminum concentration

Wang, Huahua; Hou, Junjie; Li, Yan; Zhang, Yangyang; Huang, Junjun; Liang, Weihong

doi:10.1007/s11104-017-3197-x

Cited by 35 publications

(18 citation statements)

References 44 publications

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“…In our study, cold stress promoted ROS production ( Figure 6 A and Figure 7 A). It has been reported that cytosolic G6PDHs participate in the modulation of cellular redox states by supplying NADPH [ 26 , 36 ]. We found that the levels of ROS were obviously lower in FaG6PDH-CY overexpression than control under cold stress, and the cellular membrane damage in cold-exposed overexpression strawberry was less severe as manifested by the notable decrease in MDA content ( Figure 6 B).…”

Section: Discussionmentioning

confidence: 99%

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Zhang

Luo

Cheng

et al. 2020

IJMS

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Glucose-6-phosphate dehydrogenase (G6PDH) plays an important role in plant stress responses. Here, five FaG6PDH sequences were obtained in strawberry, designated as FaG6PDH-CY, FaG6PDH-P1, FaG6PDH-P1.1, FaG6PDH-P2 and FaG6PDH-P0, which were divided into cytosolic (CY) and plastidic (P) isoforms based on the bioinformatic analysis. The respective FaG6PDH genes had distinct expression patterns in all tissues and at different stages of fruit development. Notably, FaG6PDH-CY was the most highly expressed gene among five FaG6PDH members, indicating it encoded the major G6PDH isoform throughout the plant. FaG6PDH positively regulated cold tolerance in strawberry. Inhibition of its activity gave rise to greater cold-induced injury in plant. The FaG6PDH-CY transcript had a significant increase under cold stress, similar to the G6PDH enzyme activity, suggesting a principal participant in response to cold stress. Further study showed that the low-temperature responsiveness (LTR) element in FaG6PDH-CY promoter can promote the gene expression when plant encountered cold stimuli. Besides, FaG6PDH-CY was involved in regulating cold-induced activation of antioxidant enzyme genes (FaSOD, FaCAT, FaAPX and FaGR) and RBOH-dependent ROS generation. The elevated FaG6PDH-CY enhanced ROS-scavenging capability of antioxidant enzymes to suppress ROS excessive accumulation and relieved the oxidative damage, eventually improving the strawberry resistance to cold stress.

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Section: Discussionmentioning

confidence: 99%

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Zhang

Luo

Cheng

et al. 2020

IJMS

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“…Lipid peroxidation was estimated via determining the content of malondialdehyde (MDA) according to Wang et al (2017) . Fresh rice roots (about 0.2 g) were ground and extracted in 2 mL of 10% ( w / v ) trichloroacetic acid; then the supernatant was collected by centrifuging at 8,000 × g for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…The residue was dissolved in 4 mL of 2 M H 2 SO 4 . After 5 min of incubation at 25°C, the absorbance was recorded at 415 nm ( Wang et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…Increasing the activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and catalase (CAT) to alleviate damage from ROS is a common way in which Al toxicity is alleviated in plants ( Wang and Yang, 2005 ; Zhang et al, 2010b ). Wang et al (2017) recently found that the activity of cytosolic glucose-6-phosphate dehydrogenase is also involved in resistance to Al through mediating ROS levels in soybean ( Wang et al, 2017 ). In addition, plants alleviate Al toxicity by combining Al with organic compounds to detoxify it in the cytoplasm, for example, combining Al with citrate in buckwheat or with delphinidin-3-glucoside and 3-caffeolylquinic acid in Hydrangea macrophylla ( Ma et al, 1998 ; Ma and Hiradate, 2000 ; Schreiber et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice

et al. 2018

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Hydrogen sulfide (H2S) plays a vital role in Al3+ stress resistance in plants, but the underlying mechanism is unclear. In the present study, pretreatment with 2 μM of the H2S donor NaHS significantly alleviated the inhibition of root elongation caused by Al toxicity in rice roots, which was accompanied by a decrease in Al contents in root tips under 50 μM Al3+ treatment. NaHS pretreatment decreased the negative charge in cell walls by reducing the activity of pectin methylesterase and decreasing the pectin and hemicellulose contents in rice roots. This treatment also masked Al-binding sites in the cell wall by upregulating the expression of OsSATR1 and OsSTAR2 in roots and reduced Al binding in the cell wall by stimulating the expression of the citrate acid exudation gene OsFRDL4 and increasing the secretion of citrate acid. In addition, NaHS pretreatment decreased the symplasmic Al content by downregulating the expression of OsNRAT1, and increasing the translocation of cytoplasmic Al to the vacuole via upregulating the expression of OsALS1. The increment of antioxidant enzyme [superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POD)] activity with NaHS pretreatment significantly decreased the MDA and H2O2 content in rice roots, thereby reducing the damage of Al3+ toxicity on membrane integrity in rice. H2S exhibits crosstalk with nitric oxide (NO) in response to Al toxicity, and through reducing NO content in root tips to alleviate Al toxicity. Together, this study establishes that H2S alleviates Al toxicity by decreasing the Al content in the apoplast and symplast of rice roots.

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“…− Staining H 2 O 2 and O 2 − staining was carried out according to the method described by Wang et al [47].…”

Section: H 2 O 2 and Omentioning

confidence: 99%

Alternative Pathway is Involved in Nitric Oxide-Enhanced Tolerance to Cadmium Stress in Barley Roots

Wang

Feng

et al. 2019

Plants

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Alternative pathway (AP) has been widely accepted to be involved in enhancing tolerance to various environmental stresses. In this study, the role of AP in response to cadmium (Cd) stress in two barley varieties, highland barley (Kunlun14) and barley (Ganpi6), was investigated. Results showed that the malondialdehyde (MDA) content and electrolyte leakage (EL) level under Cd stress increased in two barley varieties. The expressions of alternative oxidase (AOX) genes (mainly AOX1a), AP capacity (Valt), and AOX protein amount were clearly induced more in Kunlun14 under Cd stress, and these parameters were further enhanced by applying sodium nitroprussid (SNP, a NO donor). Moreover, H2O2 and O2− contents were raised in the Cd-treated roots of two barley varieties, but they were markedly relieved by exogenous SNP. However, this mitigating effect was aggravated by salicylhydroxamic acid (SHAM, an AOX inhibitor), suggesting that AP contributes to NO-enhanced Cd stress tolerance. Further study demonstrated that the effect of SHAM application on reactive oxygen species (ROS)-related scavenging enzymes and antioxidants was minimal. These observations showed that AP exerts an indispensable function in NO-enhanced Cd stress tolerance in two barley varieties. AP was mainly responsible for regulating the ROS accumulation to maintain the homeostasis of redox state.

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Nitric oxide-mediated cytosolic glucose-6-phosphate dehydrogenase is involved in aluminum toxicity of soybean under high aluminum concentration

Cited by 35 publications

References 44 publications

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Identification of the Cytosolic Glucose-6-Phosphate Dehydrogenase Gene from Strawberry Involved in Cold Stress Response

Hydrogen Sulfide Alleviates Aluminum Toxicity via Decreasing Apoplast and Symplast Al Contents in Rice

Alternative Pathway is Involved in Nitric Oxide-Enhanced Tolerance to Cadmium Stress in Barley Roots

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