Nitrite assimilation by the yeast <i>Candida nitratophila</i>

Kubisi, Adnan Al; Ali, A. H.; Hipkin, C. R.

doi:10.1111/j.1469-8137.1996.tb01851.x

Cited by 8 publications

(9 citation statements)

References 9 publications

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“…As for the fermentation medium with 0.01% (w/v) nitrogen content, potassium nitrate, KNO 3 showed the highest yield among the other sources, 26.6% of biomass yield increment compare to the control without any supplements (Figure 4). According to Kubisi et al (1996), potassium nitrate is a naturally occurring mineral source of nitrogen, but only several yeasts are able to assimilate nitrate as a nitrogen source. Candida is one the species which its physiology and enzymology of nitrate assimilation of some species in general.…”

Section: Resultsmentioning

confidence: 99%

Effects of nitrogen supplementation on yeast (Candida utilis) biomass production by using pineapple (Ananas comosus) waste extracted medium

Ahmad¹,

Cheong²

2007

MJM

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Pineapple waste medium was used to cultivate yeast, Candida utilis. It served as the sole carbon and energy source for the yeast growth. However, pineapple waste media contain very little nitrogen (0.003-0.015% w/v). Various nitrogen sources were incorporate and their effects on biomass, yield and productivity were studied. Significant (p<0.05) increment on biomass production was observed when nitrogen supplement (commercial yeast extract, peptone, ammonium dihydrogen phosphate, ammonium sulphate and potassium nitrate) was added into fermentation medium. Commercial yeast extract, Maxarome ® which increased 55.2% of biomass production at 0.09% (w/v) nitrogen content, is the most suitable among the selected organic source. On the other hand, ammonium dihydrogen phosphate at 0.09% (w/v) nitrogen content is comparable inorganic source which enhanced 53.7% of production. Total nitrogen content of each treatment at 0.05% (w/v) showed that nitrogen supplied was not fully utilized as substrate limitation in the fermentation medium.

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Section: Resultsmentioning

confidence: 99%

Effects of nitrogen supplementation on yeast (Candida utilis) biomass production by using pineapple (Ananas comosus) waste extracted medium

Ahmad¹,

Cheong²

2007

MJM

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“…But Boeckstaens et al (2007) also suggested that cells were unable to prevent ammonia (NH 3 ) diffusing through the plasma membrane and that released NH 3 was reabsorbed as ammonium by the nonselective cation transporters involved in its release. Al Kubisi et al (1996) found that the yeast Candida nitratophila was able to reduce nitrite to ammonium but released it into the growth media without further assimilation. It is possible that uncontrolled loss of or NH 3 by Lactarius species in our study may have restricted biomass production.…”

Section: Figmentioning

confidence: 99%

Growth on nitrate and occurrence of nitrate reductase‐encoding genes in a phylogenetically diverse range of ectomycorrhizal fungi

et al. 2008

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Summary• Ectomycorrhizal (ECM) fungi are often considered to be most prevalent under conditions where organic sources of N predominate. However, ECM fungi are increasingly exposed to nitrate from anthropogenic sources. Currently, the ability of ECM fungi to metabolize this nitrate is poorly understood.• Here, growth was examined among 106 isolates, representing 68 species, of ECM fungi on nitrate as the sole N source. In addition, the occurrence of genes coding for the nitrate reductase enzyme (nar gene) in a broad range of ectomycorrhizal fungi was investigated.• All isolates grew on nitrate, but there was a strong taxonomic signature in the biomass production, with the Russulaceae and Amanita showing the lowest growth. Thirty-five partial nar sequences were obtained from 43 tested strains comprising 31 species and 10 genera. These taxa represent three out of the four clades of the Agaricales within which ECM fungi occur. No nar sequences were recovered from the Russulaceae and Amanita, but Southern hybridization showed that the genes were present.• The results demonstrate that the ability to utilize nitrate as an N source is widespread in ECM fungi, even in those fungi from boreal forests where the supply of nitrate may be very low.

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“…The physiology and enzymology of nitrate assimilation have been extensively studied in nitrophilous yeasts, such as Candida , and Rhodotorula [ 10 ], as well as Wickerhamomyces and Ogataea (formerly Hansenula ) [ 11 ] . Nitrophilous yeasts exhibit rapid growth at concentrations higher than 10 mM nitrate-nitrogen, while similar nitrate concentrations are usually toxic, inhibiting yeast growth [ 12 ]. On the contrary, tolerance to high nitrite concentrations has been rarely documented.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Phylogenetic Characterization of Rhodotorula diobovata DSBCA06, a Nitrophilous Yeast

Civiero

Pintus

Ruggeri

et al. 2018

Biology

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Agriculture and intensive farming methods are the greatest cause of nitrogen pollution. In particular, nitrification (the conversion of ammonia to nitrate) plays a role in global climate changes, affecting the bio-availability of nitrogen in soil and contributing to eutrophication. In this paper, the Rhodotorula diobovata DSBCA06 was investigated for growth kinetics on nitrite, nitrate, or ammonia as the sole nitrogen sources (10 mM). Complete nitrite removal was observed in 48 h up to 10 mM initial nitrite. Nitrogen was almost completely assimilated as organic matter (up to 90% using higher nitrite concentrations). The strain tolerates and efficiently assimilates nitrite at concentrations (up to 20 mM) higher than those previously reported in literature for other yeasts. The best growth conditions (50 mM buffer potassium phosphate pH 7, 20 g/L glucose as the sole carbon source, and 10 mM nitrite) were determined. In the perspective of applications in inorganic nitrogen removal, other metabolic features relevant for process optimization were also evaluated, including renewable sources and heavy metal tolerance. Molasses, corn, and soybean oils were good substrates, and cadmium and lead were well tolerated. Scale-up tests also revealed promising features for large-scale applications. Overall, presented results suggest applicability of nitrogen assimilation by Rhodotorula diobovata DSBCA06 as an innovative tool for bioremediation and treatment of wastewater effluents.

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Nitrite assimilation by the yeast Candida nitratophila

Cited by 8 publications

References 9 publications

Effects of nitrogen supplementation on yeast (Candida utilis) biomass production by using pineapple (Ananas comosus) waste extracted medium

Effects of nitrogen supplementation on yeast (Candida utilis) biomass production by using pineapple (Ananas comosus) waste extracted medium

Growth on nitrate and occurrence of nitrate reductase‐encoding genes in a phylogenetically diverse range of ectomycorrhizal fungi

Physiological and Phylogenetic Characterization of Rhodotorula diobovata DSBCA06, a Nitrophilous Yeast

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