Nitrite causes reversible inactivation of nitrate reductase in the yeast Hansenula anomala

González, Celedonio; González, Gregorio; Ávila, Julio; Pérez, Montse; Brito, Nélida; Siverio, José M.

doi:10.1099/00221287-140-10-2633

Cited by 10 publications

(10 citation statements)

References 16 publications

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“…1 at around 4–8 h where the rate of nitrogen production was temporarily reduced until the nitrite levels in the medium began to fall. González et al . (1994) have previously demonstrated the inactivation of nitrate reductase by nitrite in the yeast Hansenula anomala.…”

Section: Discussionmentioning

confidence: 99%

Effects of cultural conditions on denitrification by Pseudomonas stutzeri measured by Membrane Inlet Mass Spectrometry

Firth¹,

Edwards

1999

J Appl Microbiol

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. 1999. Denitrification is a globally important process leading to loss of fertiliser efficiency and the production of the greenhouse gas nitrous oxide and nitric oxide, an ozone depleter. Membrane inlet mass spectrometry (MIMS) was employed to study the effect of different variables on the process of denitrification by Pseudomonas stutzeri in a defined salts medium. MIMS was used for concomitant measurements of nitrous oxide, nitrogen and oxygen and showed that denitrification occurred in the presence of dissolved oxygen. A nitrate concentration of 15 mmol l −1 and a nitrite concentration of 5 mmol l −1 were found to be optimum for complete denitrification of nitrate or nitrite to nitrogen and varying these concentrations had a marked effect on the ratio of gaseous products released. Denitrification products were also dependant on pH with neutral or alkaline conditions being best for production of gaseous end products. Our results suggest that under nutrient rich conditions the most important factor in the regulation of denitrification by Ps. stutzeri is the amount of nitrite generated at the first enzymatic stage of the process. This appears to cause inhibition of the denitrification pathway above 5 mmol l −1 and at high enough concentrations (15 mmol l −1 ) restricts growth.

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Section: Discussionmentioning

confidence: 99%

Effects of cultural conditions on denitrification by Pseudomonas stutzeri measured by Membrane Inlet Mass Spectrometry

Firth¹,

Edwards

1999

J Appl Microbiol

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“…This inactivation is reversible and does not affect a non‐physiological activity of the protein in which only the molybdopterin moiety is involved. Curiously, the inactive enzyme was found to be associated with membranes presumably from mitochondria [67,68]. A satisfactory explanation to this localisation has not yet been found; however, a similar situation has been found in plants [69,70].…”

Section: Non‐transcriptional Regulation Of the Proteins Involved In mentioning

confidence: 92%

“…In H. anomala the absence of nitrogen sources or the presence of ammonium or glutamine produces a loss of nitrate reductase activity and protein [7,66]. In addition, in this yeast nitrate reductase undergoes inactivation as a response to nitrite, uncouplers and heat shock [67,68]. This inactivation is reversible and does not affect a non‐physiological activity of the protein in which only the molybdopterin moiety is involved.…”

Section: Non‐transcriptional Regulation Of the Proteins Involved In mentioning

confidence: 99%

Assimilation of nitrate by yeasts

Siverio

2002

FEMS Microbiol Rev

114

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Nitrate assimilation has received much attention in filamentous fungi and plants but not so much in yeasts. Recently the availability of classical genetic and molecular biology tools for the yeast Hansenula polymorpha has allowed the advance of the study of this metabolic pathway in yeasts. The genes YNT1, YNR1 and YNI1, encoding respectively nitrate transport, nitrate reductase and nitrite reductase, have been cloned, as well as two other genes encoding transcriptional regulatory factors. All these genes lie closely together in a cluster. Transcriptional regulation is the main regulatory mechanism that controls the levels of the enzymes involved in nitrate metabolism although other mechanisms may also be operative. The process involved in the sensing and signalling of the presence of nitrate in the medium is not well understood. In this article the current state of the studies of nitrate assimilation in yeasts as well as possible venues for future research are reviewed.

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“…Nitrite, an intermediate in the reductive assimilation of nitratc-nitrogen into ammonium-nitrogen, is also a potential source of nitrogen for these organisms and appears to play an important role in the regulation of nitrate assimilation (Gonzalez et al, 1994). However, although nitrite uptake has been studied in cyanobacteria and algae (Cresswell &: Syrett, 1982;Flores, Herrero & Guerrero.…”

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confidence: 99%

Nitrite assimilation by the yeast Candida nitratophila

1996

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S L: M M A R yNitrite uptake in the nitrate-assimilating yeast, Candida nitratophila, was inhibited completely by addition of nitrate or ammonium to cultures at pH SO. Nitrite assimilation m these cultures was also completely dependent on the availability of a suitable carbon source (glucose). In cultures at pH 5'0, neither nitrate nor ammonium inhibited nitrite uptake completely. In these cultures, some nitrite was assimilated in the absence of a carbon source. Carbon-deficient cultures at pH 5'0 were able to reduce nitrite to ammonium but excreted this ammonium without further assimilation. E\idence for the uptake of nitrite by passive diffusion and by active transport via the nitrate uptake system is discussed.

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Nitrite causes reversible inactivation of nitrate reductase in the yeast Hansenula anomala

Abstract: The addition of nitrite, the product of the reaction catalysed by nitrate reductase, to cell suspensions of the yeast Hansenula anomala caused a reversible inactivation of NADPH-dependent nitrate reductase activity. The

Cited by 10 publications

References 16 publications

Effects of cultural conditions on denitrification by Pseudomonas stutzeri measured by Membrane Inlet Mass Spectrometry

Effects of cultural conditions on denitrification by Pseudomonas stutzeri measured by Membrane Inlet Mass Spectrometry

Assimilation of nitrate by yeasts

Nitrite assimilation by the yeast Candida nitratophila

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