Nitrogen, carbon and light-mediated regulation studies of carotenoid biosynthesis in immobilized mycelia of Gibberella fujikuroi

Garbayo, Inés; Vı́lchez, C.; Nava-Saucedo, José-Edmundo; Barbotin, Jean‐Noël

doi:10.1016/s0141-0229(03)00182-0

Cited by 42 publications

(26 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As in F. fujikuroi, this activation was independent of that produced by light, as indicated by the similar result obtained with wc mutants, totally devoid of photocarotenogenesis. A different approach led to similar conclusions in G. fujikuroi (19). Immobilized mycelia incubated under nitrogen starvation in the dark increased their carotenoid content for several days, but addition of nitrogen resulted in cessation of the synthesis.…”

Section: Discussionmentioning

confidence: 71%

“…An earlier work described that nitrogen availability affects carotenoid production in immobilized G. fujikuroi mycelia (19). To evaluate the relationship between carotenoid biosynthesis and the production of nitrogen-regulated secondary metabolites in F. fujikuroi, we analyzed the effect of nitrogen starvation on carotenogenesis in parallel to gibberellin and bikaverin production in the wild type and in carS mutants.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Rodríguez-Ortiz

Limón

Ávalos

2009

Appl Environ Microbiol

View full text Add to dashboard Cite

The fungus Fusarium fujikuroi (Gibberella fujikuroi MP-C) produces metabolites of biotechnological interest, such as gibberellins, bikaverins, and carotenoids. Gibberellin and bikaverin productions are induced upon nitrogen exhaustion, while carotenoid accumulation is stimulated by light. We evaluated the effect of nitrogen availability on carotenogenesis in comparison with bikaverin and gibberellin production in the wild type and in carotenoid-overproducing mutants (carS). Nitrogen starvation increased carotenoid accumulation in all strains tested. In carS strains, gibberellin and bikaverin biosynthesis patterns differed from those of the wild type and paralleled the expression of key genes for both pathways, coding for geranylgeranyl pyrophosphate (GGPP) and kaurene synthases for the former and a polyketide synthase for the latter. These results suggest regulatory connections between carotenoid biosynthesis and nitrogen-controlled biosynthetic pathways in this fungus. Expression of gene ggs1, which encodes a second GGPP synthase, was also derepressed in the carS mutants, suggesting the participation of Ggs1 in carotenoid biosynthesis. The carS mutations did not affect genes for earlier steps of the terpenoid pathway, such as fppS or hmgR. Light induced carotenoid biosynthesis in the wild type and carRA and carB levels in the wild-type and carS strains irrespective of nitrogen availability.

show abstract

Section: Discussionmentioning

confidence: 71%

mentioning

confidence: 99%

Regulation of Carotenogenesis and Secondary Metabolism by Nitrogen in Wild-Type Fusarium fujikuroi and Carotenoid-Overproducing Mutants

Rodríguez-Ortiz

Limón

Ávalos

2009

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Studies have demonstrated that the fungus Gibberella fujikuroi is influenced by light in its mycelial growth, in the presence of light there is production of orange carotenoids and in the dark there is no carotenoids production (Garbayo et al, 2003). As previously mentioned, the production of carotenoids is influenced by factors such as light, pH, temperature and culture medium (Burja et al, 2006;Ramirez et al, 2001;Santos et al, 2016).…”

Section: Carotenoids Produced By Yeasts and Filamentous Fungimentioning

confidence: 97%

“…Usually the fungi grow at temperatures between 25 and 30°C (Garbayo et al, 2003;Estrada et al, 2009;Csernetics et al, 2011). Studies have demonstrated that the fungus Gibberella fujikuroi is influenced by light in its mycelial growth, in the presence of light there is production of orange carotenoids and in the dark there is no carotenoids production (Garbayo et al, 2003).…”

Section: Carotenoids Produced By Yeasts and Filamentous Fungimentioning

confidence: 99%

Microbial production of carotenoids A review

Cardoso¹,

Kanno²,

Karp³

2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Carotenoids are natural pigments that can be synthesized by various microorganisms, including bacteria, yeasts, filamentous fungi and microalgae. These pigments comprise around 700 different structures with peculiar colors and biological properties that are beneficial to health. Advantages of biotechnological production of carotenoids include the ability of microorganisms to use low cost substrates, the optimized control of cultivation, minimized production time and the natural origin of the synthesized pigments. Techniques for separation and purification of carotenoids are well established at laboratory scale, however the development of processes that can be economically scaled-up is essential for industrial production.

show abstract

“…Neurosporaxanthin biosynthesis in F. fujikuroi is stimulated by nitrogen starvation: immobilized mycelia incubated under low nitrogen conditions produce carotenoids, but addition of nitrogen slows down the synthesis [ 227 ]. Moreover, the exchange of immobilized mycelia between media with different nitrogen contents results in induction or cessation of carotene synthesis, depending on the absence or presence of nitrogen, respectively.…”

Section: Dependence On Temperature and Nitrogenmentioning

confidence: 99%