Nitrogen Removal Characteristics and Comparison of the Microbial Community Structure in Different Anaerobic Ammonia Oxidation Reactors

Zhang, Liqiu; Lv, Wei; Li, Shugeng; Geng, Zhongxuan; Yao, Hainan

doi:10.3390/w11020230

Cited by 7 publications

(3 citation statements)

References 22 publications

(23 reference statements)

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“…The culturable microorganism accounts for 1% ~ 5%; therefore, molecular biology has been widely used in microbial community research. Lang et al and Zhang et al studied the community structure of constructed wetlands using molecular biology methods ( Zhang et al, 2019 ; Lang et al, 2021 ). This study was the first to investigate the composition and distribution characteristics of Massilia in sewage, substrate, plant rhizosphere, plant phyllosphere and air in a constructed wetland sewage treatment system by two molecular biology methods: construction of a 16S rDNA clone library and RFLP sequence analysis.…”

Section: Introductionmentioning

confidence: 99%

Dynamic distribution of Massilia spp. in sewage, substrate, plant rhizosphere/phyllosphere and air of constructed wetland ecosystem

Liu

Zhao³

et al. 2023

Front. Microbiol.

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IntroductionMassilia bacteria are widely distributed and have various ecological functions. Preliminary studies have shown that Massilia is the dominant species in constructed wetland ecosystems, but its species composition and distribution in constructed wetlands are still unclear.MethodsIn this paper, the in-house-designed primers were used to construct a 16S rDNA clone library of Massilia. The RFLP sequence analysis method was used to analyze the diversity of Massilia clone library and the composition of Massilia in sewage, substrate, plant rhizosphere, plant phyllosphere and air in a constructed wetland sewage treatment system. Redundancy analysis (RDA) and canonical correspondence analysis (CCA) were used to analyze the correlation between environmental factors and the population characteristics of Massilia in the corresponding environment. The dominant species of Massilia were analyzed for differences.ResultsThe results showed that the 16S rDNA clone library in primer 5 worked well. According to the clone library diversity index analysis, the richness of Massilia varied significantly in different environments in different seasons, where the overall summer and autumn richness was higher than that in the spring and winter. The relative abundance of 5 Massilia in the constructed wetland ecosystem was greater than 1% in all samples, which were M. alkalitolerans, M. albidiflava, M. aurea, M. brevitalea, and M. timonae. The seasonal variation of dominant genera was significantly correlated with environmental factors in constructed wetlands.DiscussionThe above results indicated that the species of Massilia were abundant and widely distributed in the constructed wetland ecosystem, and there were significant seasonal differences. In addition, the Massilia clone library of constructed wetland was constructed for the first time in this study and the valuable data of Massilia community structure were provided, which was conducive to the further study of microbial community in constructed wetland.

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Section: Introductionmentioning

confidence: 99%

Dynamic distribution of Massilia spp. in sewage, substrate, plant rhizosphere/phyllosphere and air of constructed wetland ecosystem

Liu

Zhao³

et al. 2023

Front. Microbiol.

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“…Most previous fundamental studies about PN/A process have focused solely on the microbial ecology of the autotrophs including AOB, AMX and nitrite-oxidizing bacteria (NOB) [4][5][6][7]. However, the other communities, for instance heterotrophs in this system, have often been neglected.…”

Section: Introductionmentioning

confidence: 99%

Evaluating Microbial Interactions of Autotrophs and Heterotrophs in Partial Nitritation/Anammox (PN/A) Process by Experimental and Simulation Analyses

Lai

Chu

Huang

et al. 2021

Water

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The microbial interactions between autotrophs and heterotrophs by the exchange of microbial products in a partial nitritation/anammox (PN/A) bioreactor were evaluated with both experimental and simulation analyses. Real-time quantitative PCR analysis showed that anammox bacteria (AMX) and ammonium oxidizing bacteria (AOB) made up 56.59% and 8.35% of total bacteria, respectively, while heterotrophs identified as Chloroflexi also constituted a large portion (32.76%) in the reactor, even without an external organic carbon supply. Furthermore, a mathematical model was developed to describe the growth of heterotrophs on soluble microbial products (SMP), which were released from the metabolism of autotrophs. After model calibration and validation, the simulation results were consistent with the experimental observations of the microbial composition and the nitrogenous transformations. According to the model analysis, the bulk oxygen concentration was determined to be the dominant factor governing the reactor performance and biomass fractions in the granule. Increasing granular size could decrease heterotrophic growth, but has little effect on the effluent concentration of SMP. Results of this study could establish a better understanding of eco-physiological interactions of autotrophs and heterotrophs in PN/A process.

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“…During sewage treatment, the sewage flows continuously along an open curve under the action of gravity, and the curve is divided into multiple sections. The water quality parameters change continuously along the direction of the water flow but do not change with time, thus providing a stable environment for the growth of microorganisms [16,17]. After the operation stabilizes, theoretically, succession occurs in the microbial community structure of different biofilms, and the metabolic function interval is eliminated.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Removal Efficiency and Microbial Community Analysis of a High-Efficiency Honeycomb Fixed-Bed Bioreactor

Zhu

Liu

et al. 2020

Water

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Based on the concept of microbial community multi-processing in integrated spatial bacterial succession (ISBS), this study constructs a highly efficient cellular fixed-bed bioreactor that follows the growth of biological flora in the wastewater treatment process. The reactor is organically partitioned based on synergistic laws and in accordance with environmental and microbial metabolic changes, and sewage is subjected to unitized and specialized biological treatment under direct current conditions. The results show that the ISBS reactor exhibits stable nitrogen removal performance under a low-carbon source. Compared with traditional sewage biochemical treatment technology, the microbial concentration is increased by 2–3 times and even up to 12 times, and the ammonia nitrogen removal rate is maintained at 99%. The removal rate reaches 90% (hydraulic retention time of 14 h). High-throughput sequencing analysis based on 16S rDNA reveals the microbial community structure succession at different depths of the same section of the reactor. The microbial community is rich under the influence of environmental factors and exhibits different responses. The intervals vary. An analysis of the microbial community function explains why the ISBS reactor has high nitrogen removal efficiency.

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Nitrogen Removal Characteristics and Comparison of the Microbial Community Structure in Different Anaerobic Ammonia Oxidation Reactors

Cited by 7 publications

References 22 publications

Dynamic distribution of Massilia spp. in sewage, substrate, plant rhizosphere/phyllosphere and air of constructed wetland ecosystem

Dynamic distribution of Massilia spp. in sewage, substrate, plant rhizosphere/phyllosphere and air of constructed wetland ecosystem

Evaluating Microbial Interactions of Autotrophs and Heterotrophs in Partial Nitritation/Anammox (PN/A) Process by Experimental and Simulation Analyses

Nitrogen Removal Efficiency and Microbial Community Analysis of a High-Efficiency Honeycomb Fixed-Bed Bioreactor

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