Nocardioides cynanchi sp. nov., isolated from soil of rhizosphere of Cynanchum wilfordii

Abstract

A novel actinobacterial strain, SB3-45T, was isolated from soil of Cynanchum wilfordii rhizosphere, Jaecheon-si, Chungcheongbuk-do, Republic of Korea. Strain SB3-45T, was Gram-stain-positive, aerobic and coccoid to short rod-shaped bacterium. Growth occurred at 4–37 °C (optimum 28 °C), pH 5–8 (optimum pH 7) and 0–2.5 % NaCl (optimum 0%). Phylogenetic analysis based on 16S rRNA gene sequence showed that strain SB3-45T belonged to the genus Nocardioides and was closely related to Nocardioides opuntiae OS-21T (96.2%) and Nocardioides panacihumi Gsoil 616T (95.9%). ll-DAP as the diamino acid in the peptidoglycan and the menaquinone MK-8(H4) as the predominant isoprenoid quinone were detected. The polar lipids of strain SB3-45T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unidentified phospholipid. The major cellular fatty acids (>5%) of strain SB3-45T were iso-C16 : 0, C18 : 1 ω9c and C17 : 0. Based on phylogenetic, physiological and chemotaxonomic characteristics, strain SB3-45T represents a novel species of the genus Nocardioides, for which the name Nocardioides cynanchi sp.nov. is proposed. The type strain is SB3-45T (=KCTC 49133T=NBRC 114107T).

Nocardioides panacis sp. nov., isolated from soil of a ginseng field

“…S5). The strain had menaquinone with eight isoprene units (MK-8) as the major quinone, consistent with other members of the genus Nocardioides [3–8], and ll -diaminopimelic acid as the diagnostic diamino acid of the cell-wall peptidoglycan.…”

“…At the time of writing, the genus consists of 124 species with validly published names (https://lpsn.dsmz.de/genus/nocardioides). Nocardioides species have been isolated from soil of various environments, such as forest, land without vegetation, mountain, ginseng field, and rhizosphere of Cynanchum wilfordii [2][3][4][5][6]. According to phylogenetic analysis of 16S rRNA gene sequences, the genus Nocardioides is closely related to the genus Marmoricola [7]; however, there are remarkable differences in their fatty acid profiles, including the amount of iso-branched-chain fatty acids.…”

Microbial features of mature and abandoned soils in refractory clay deposits

“…Plant-associated microorganisms were founded previously in rhizospheres -for example, Bradyrhyzobium [23], Nocardioides [32]. Some of the taxa previously reported as typical soil microbiota -Nocardioides [32,33], Pseudonocardia [34,35], Kribbella [36], Pseudolabrys [37]. Except differential analysis limitation, this result can be connected with using of topsoil.…”

“…Due differential abundance analysis limitations, mainly common soil-and plant-associated taxa were found. Plant-associated microorganisms were founded previously in rhizospheres -for example, Bradyrhyzobium [23], Nocardioides [32]. Some of the taxa previously reported as typical soil microbiota -Nocardioides [32,33], Pseudonocardia [34,35], Kribbella [36], Pseudolabrys [37].…”

Nocardioides mangrovi sp. nov., a novel endophytic actinobacterium isolated from root of Kandelia candel

“…Among these, the recently reported Nocardioides were separated from a variety of environments, such as Acrostichum aureum, Tagetes patula, soil of rhizosphere, tibetan antelope, dandelion, sewage sludge, cave, etc. [2][3][4][5][6][7]. Members of the genus Nocardioides are characterized as being Gram-positive and aerobic with a high DNA G+C content (68.7-74.9 mol%).…”