Nocardioides deserti sp. nov., an actinobacterium isolated from desert soil

Li, Tuo; Dong, Yanhong; Habden, Xugela; Liu, Jiameng; Guo, Lin; Liu, Xianfu; Chen, Li; Jiang, Zhong-Ke; Liu, Shaowei; Zhang, Yubin; Zhang, Yuqin; Sun, Cheng‐Hang

doi:10.1099/ijs.0.000147

Cited by 44 publications

(24 citation statements)

References 43 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the analysis of whole-cell fatty acids, cell mass of strain IP6SC6 T and P. jejuensis NRRL B-24460 T were harvested from both TSA and R2A agar plates grown at 28 8C for 3 days, when the bacterial communities reached the late-exponential stage of growth according to the four quadrants streak method. The whole-cell fatty acids were saponified, methylated and extracted according to the standard protocol described by Sasser (1990), and analysed according to the method described by Tuo et al (2015). For G+C content assessment, genomic DNA of strain IP6SC6 T was prepared according to the method described by Marmur (1961) and the G+C content was determined by reversed-phase HPLC as described by Mesbah et al (1989).…”

mentioning

confidence: 99%

Phycicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from Bruguiera gymnorhiza

Liu

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

A novel endophytic actinobacterium, designated strain IP6SC6 T , was isolated from surfacesterilized bark of Bruguiera gymnorhiza collected from Zhanjiang Mangrove Forest National Nature Reserve in Guangdong, China. Cells of strain IP6SC6 T were Gram-stain-positive, aerobic, non-spore-forming, non-motile rods. Strain IP6SC6 T grew at 20-42 8C (optimum, 37 8C), at pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-8 % (w/v) NaCl (optimum, 0-2 %). Chemotaxonomic analyses showed that the isolate possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan, galactose and glucose as whole-cell sugars, and MK-8(H 4 ) as the predominant menaquinone. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and an unknown lipid. The major fatty acids were iso-C 15 : 0 , anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 . The G+C content of the genomic DNA was 72.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain IP6SC6 T belonged to the genus Phycicoccus and shared the highest sequence similarity with Phycicoccus jejuensis NRRL B-24460 T (96.97 %). On the basis of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain IP6SC6 T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus endophyticus sp. nov. is proposed. The type strain is IP6SC6 T (5DSM 100020 T 5CGMCC 4.7300 T ).

show abstract

mentioning

confidence: 99%

Phycicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from Bruguiera gymnorhiza

Liu

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Members of this genus are Gramstaining positive, non-acid-fast, aerobic and motile or non motile actinomycetes (Evtushenko et al 2015). The genus Nocardioides is versatile, being isolated not only from soil (Prauser 1976;Ahn et al 2014), but also from sea water (Kim et al 2008), volcanic ash (Lee and Lee 2014), sea food samples (Lin et al 2015), desert (Tuo et al 2015), ground water (Yoon et al 2004), beach sand (Lee et al 2007) and sediment sample (Dastager et al 2009). Different species of the genus Nocardioides have the ability to grow from psychrophilic to mesophilic (Dastager et al 2010) and thermophilic growth conditions.…”

Section: Introductionmentioning

confidence: 98%

Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan

Amin

Ahmed

Habib

et al. 2016

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated as NCCP-1340(T), was isolated from a hot water spring, Tatta Pani, Pakistan. Cells of strain NCCP-1340(T) were observed to be aerobic, rod shaped, catalase and urease positive but H2S production and oxidase negative. Growth was observed at pH 6.0-8.0 (optimum pH 7.0) and at 20-40 °C (optimum 37 °C). The strain could tolerate 0-8 % NaCl (optimum 2 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NCCP-1340(T) belongs to the genus Nocardioides and is closely related to Nocardioides iriomotensis JCM 17985(T) (96.8 %), Nocardioides daedukensis KCTC 19601(T) (96.6 %), Nocardioides jensenii KCTC 9134(T) (96.1 %) and Nocardioides daejeonensis KCTC 19772(T) (96.1 %). The DNA-DNA relatedness values of strain NCCP-1340(T) with N. iriomotensis JCM 17985(T), N. daedukensis KCTC 19601(T) and N. jensenii KCTC 9134(T) were found to be less than 53 %. The DNA G+C content of strain NCCP-1340(T) was determined to be 71.8 mol %. The affiliation of strain NCCP-1340(T) to the genus Nocardioides was further supported by chemotaxonomic data which showed the presence of MK-8(H4) as major menaquinone system; iso-C16:0, C17:0, C16:0 10-methyl, iso-C15:0 and C 15:0 as major cellular fatty acids; and diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and unidentified glycolipids and polar lipids in the polar lipids profile. The cell wall peptidoglycan contained LL-diaminopimelic acid as the diagnostic amino acid. On the basis of physiological and biochemical characteristics and the phylogenetic analyses, strain NCCP-1340(T) can be distinguished from the closely related taxa and thus represents a novel species of the genus Nocardioides, for which the name Nocardioides pakistanensis sp. nov. is proposed with the type strain NCCP-1340(T) (= DSM 29942(T) = JCM 30630(T)).

show abstract

“…html). The sources for these strains include black sand (Lee, 2007), marine sediment (Deng et al, 2015), ground water (Yoon et al, 2004), seawater (Choi et al, 2007), sea urchins (Lin et al, 2015) and soil, such as rhizosphere soil (Lee et al, 2011), desert soil (Tuo et al, 2015) and mountain soil (Srinivasan et al, 2014). Of these, a few have been reported as endophytes, namely Nocardioides caricicola (Song et al, 2011), Nocardioides panzhihuaensis (Qin et al, 2012), Nocardioides endophyticus (Han et al, 2013), Nocardioides conyzicola (Han et al, 2013) and Nocardioides zeae (Glaeser et al, 2014 reports another novel endophyte of the genus Nocardioides isolated from the roots of Ginkgo biloba L.…”

mentioning

confidence: 99%

Nocardioides ginkgobilobae sp. nov., an endophytic actinobacterium isolated from the root of the living fossil Ginkgo biloba L.

Zhang

Cheng

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Nocardioides ginkgobilobae sp. nov., an endophytic actinobacterium isolated from the root of the living fossil Ginkgo biloba L. A Gram-stain-positive, aerobic and yellow actinobacterial strain, designated SYP-A7303 T , was isolated from the root of Ginkgo biloba L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-A7303 T belongs to the genus Nocardioides. The 16S rRNA gene sequence of strain SYP-A7303 T showed highest similarity to Nocardioides marinus CL-DD14 T (5JCM 15615 T ) (98.3 %) and Nocardioides aquiterrae GW-9 T (5JCM 11813 T ) (97.1 %), and less than 96.9 % to the type strains of other species of the genus Nocardioides. Strain SYP-A7303 T grew optimally at 28 8C, pH 7.0 and in the absence of NaCl. It contained LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, with mannose, ribose, rhamnose, glucose and galactose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown lipid. The menaquinone was MK-8(H 4 ) and the predominant cellular fatty acids were iso-C 16 : 0 , C 18 : 1 v9c and C 17 : 1 v8c. The DNA G+C content was 72 mol%. Mean DNA-DNA relatedness values between strain SYP-A7303 T and the closely related strains N. marinus JCM 15615 T and N. aquiterrae JCM 11813 T were 62.5¡2.4 and 56.5¡3.5 %, respectively. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain SYP-A7303 T represents a novel species of the genus Nocardioides, for which the name Nocardioides ginkgobilobae sp. nov. is proposed. The type strain is SYP-A7303 T (5DSM 100492 T 5KCTC 39594 T ).Prauser (1976) introduced the generic name Nocardioides for a group of Gram-positive actinobacteria that were widely distributed in soil, with Nocardioides albus as the type species. Members of the genus are characterized by the presence of LL-2,6-diaminopimelic acid (LL-DAP) in the cell wall and MK-8(H 4 ) as the major menaquinone (O'Donnell et al., 1982;Prauser, 1976; Urzì et al., 2000). The major fatty acids of species of the genus Nocardioides are diverse, with many species containing iso-C 16 : 0 , C 17 : 1 v8c or C 18 : 1 v9c and some species having C 16 : 0 , C 17 : 0 , C 15 : 0 , iso-C 15 : 0 or iso-C 17 : 0 (Cui et al., 2009; Kim et al., 2009;Lee et al., 2011;Liu et al., 2013;Yamamura et al., 2011;Zhang et al., 2009Zhang et al., , 2012. Soon after the proposal of the genus, many novel species were added, and at the time of writing the genus is represented by 78 validly described species (http://www.bacterio.net/nocardioides. html). The sources for these strains include black sand (Lee, 2007)

show abstract

Nocardioides deserti sp. nov., an actinobacterium isolated from desert soil

Cited by 44 publications

References 43 publications

Phycicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from Bruguiera gymnorhiza

Phycicoccus endophyticus sp. nov., an endophytic actinobacterium isolated from Bruguiera gymnorhiza

Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan

Nocardioides ginkgobilobae sp. nov., an endophytic actinobacterium isolated from the root of the living fossil Ginkgo biloba L.

Contact Info

Product

Resources

About