Nocardioides marinquilinus sp. nov., isolated from coastal seawater

Abstract

A Gram-stain-positive, non-motile, strictly aerobic, non-spore-forming and short rod-shaped bacterial strain, CL-GY44 T , was isolated from coastal seawater, Korea. Analysis of the 16S rRNA gene sequence of strain CL-GY44 T revealed a clear affiliation with the genus Nocardioides. Based on phylogenetic analysis, strain CL-GY44 T showed the closest phylogenetic relationship with Nocardioides ginsengagri BX5-10 T and Nocardioides plantarum NCIMB 12834 T . Strain CL-GY44 T was not able to grow in the presence of NaCl but grew with 0-5.5 % sea salts. The optimum temperature and pH for growth were 30 6C and pH 7.0. The major cellular fatty acids of strain CL-GY44 T were C 17 : 1 v6c, iso-C 16 : 0 , C 16 : 0 and iso-C 15 : 0 and the major menaquinone was MK-8(H 4 ). The cell-wall analysis showed that strain CL-GY44 T contained LL-diaminopimelic acid. The genomic DNA G+C content was 71.6 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed that strain CL-GY44 T could be clearly distinguished from members of the genus Nocardioides. Thus, strain CL-GY44 T should be classified as representing a novel species in the genus Nocardioides, for which the name Nocardioides marinquilinus sp. nov. is proposed. The type strain is CL-GY44 T (5KCCM 90109 T 5JCM 18459 T ).The genus Nocardioides, first described by Prauser (1976), belongs to the family Nocardioidaceae, in the suborder Propionibacterineae (Zhi et al., 2009). At the time of writing, the genus Nocardioides comprised 62 species including 58 with validly published names in the List of Prokaryotic Names with Standing in Nomenclature (http://www.bacterio.cict.fr) and four species in press, with names which have been validly published during the processing of this manuscript, Nocardioides albertanoniae isolated from Roman catacombs (Alias-Villegas et al., 2013), Nocardioides perillae isolated from the surface-sterilized root of Perilla frutescens (Du et al., 2013) and Nocardioides szechwanensis and Nocardioides psychrotolerans isolated from Hailuogou glacier in Szechwan, China . Some members in the genus Nocardioides have been isolated from various environments and ten species have been discovered from various marine environments: Nocardioides aestuarii (Yi & Chun, 2004a) Here, we describe a bacterial strain, designated CL-GY44 T , isolated from coastal seawater of Gwangyang Bay, South sea of Korea. Based on the polyphasic approach, we propose that the bacterium represents a novel species in the genus Nocardioides.A sample (10 ml) of coastal seawater was spread on a 1/10-diluted marine agar (MA; Difco) and incubated at 30 u C for 3 weeks. Among 24 strains isolated from 1/10-diluted MA (12 strains of Alpha-proteobacteria, one strain of Betaproteobacteria, five strains of gammaproteobacteria, one strain of Actinobacteria, two strains of Bacteroidetes and three strains of Firmicutes), one strain assigned to the genus Nocardioides showed .3 % 16S rRNA gene sequence divergence from species of the genus Nocardioides with validly published names. A single colon...

Nocardioides salsibiostraticola sp. nov., isolated from biofilm formed in coastal seawater

“…¡96.3 %) with recognized species of the genus Nocardioides and the phylogenetic position of strain PAMC 26527 T showed that the strain could be assigned to a novel species in the genus Nocardioides. The DNA G+C content of strain PAMC 26527 T was 69.3 mol%, within the range (67-75 mol%; Prauser, 1976;Yoon et al, 2006;Cho et al, 2013) reported for recognized species of the genus Nocardioides.…”

“…Most species of the genus Nocardioides have been isolated from non-saline environments, including herbage, oil shale column, groundwater, organisms from freshwater and soil (Collins et al, 1994;Yoon et al, 1997Yoon et al, , 2004Tó th et al, 2008;Lee et al, 2012). However, 11 species have been discovered from various marine environments: Nocardioides marinus (Choi et al, 2007) and Nocardioides marinquilinus (Cho et al, 2013) from seawater, Nocardioides salarius from seawater enriched with zooplankton, Nocardioides marinisabuli , Nocardioides furvisabuli (Lee, 2007), Nocardioides hwasunensis (Lee et al, 2008), Nocardioides dokdonensis (Park et al, 2008) and Nocardioides basaltis (Kim et al, 2009) from beach sand, Nocardioides caricicola (Song et al, 2011) from a halophyte growing on a sand dune, and Nocardioides aestuarii (Yi & Chun, 2004a) and Nocardioides ganghwensis (Yi & Chun, 2004b) from tidal flat sediment.…”

Nocardioides dongxiaopingii sp. nov., isolated from leaves of Lamiophlomis rotata on the Qinghai–Tibet Plateau

“…S5) consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified phospholipids (PL1-3), one unidentified glycolipid (GL, missing in strain 4053) and one unidentified lipid (L). DPG was a major polar lipid in the isolates but absent in N. plantarum DSM 11054 T [38]; the existence of PE in N. litoris DSM 103718 T [39] and PI in N. rubriscoriae DSM 23986 T [40] was in contrast to strains S-1144 T lactose, l-arabitol, l-fucose, l-sorbose, l-xylose, maltose, melezitose, melibiose, methyl αd-glucopyranoside, methyl β-d-xylopyranoside, N-acetylglucosamine, raffinose, salicin or starch. In the API 20NE strip, weakly positive for aesculin ferric citrate hydrolysis but negative for gelatin hydrolysis, nitrate reduction, indole production and urease activity.…”

Nocardioides jishulii sp. nov.,isolated from faeces of Tibetan gazelle (Procapra picticaudata)

“…net/ nocardioides. html), which have been isolated from various sources, such as soil [2][3][4][5], seawater [6], desert [7], plants [8][9][10], lake [11] and animals [12]. In our previous studies, several novel species of the order Actinomycetales have been isolated from wild animals (Tibetan antelope, vultures and plateau pika) from the Qinghai-Tibet Plateau, including Nocardioides houyundeii [13], Actinomyces gaoshouyii [14], Actinomyces tangfeifanii [15] and Actinomyces vulturis [16].…”