Non-Conventional Methods for Gelation of Alginate

Gurikov, Pavel; Смирнова, Ирина

doi:10.3390/gels4010014

Cited by 108 publications

(70 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We developed the fast release therapy through the bioprinting fixation ( Fig. 2a) followed by freeze gelation, using ethanol 19 (Fig. 2b), enabling the fabrication of 5D nano-laden hydrogel (Fig.…”

Section: Resultsmentioning

confidence: 99%

In-vivo vascular application via ultra-fast bioprinting for future 5D personalised nanomedicine

Foresti

Rossi

Pinelli

et al. 2020

Sci Rep

View full text Add to dashboard Cite

The design of 3D complex structures enables new correlation studies between the engineering parameters and the biological activity. Moreover, additive manufacturing technology could revolutionise the personalised medical pre-operative management due to its possibility to interplay with computer tomography. Here we present a method based on rapid freeze prototyping (RFP) 3D printer, reconstruction cutting, nano dry formulation, fast freeze gelation, disinfection and partial processes for the 5D digital models functionalisation. We elaborated the high-resolution computer tomography scan derived from a complex human peripheral artery and we reconstructed the 3D model of the vessel in order to obtain and verify the additive manufacturing processes. Then, based on the drug-eluting balloon selected for the percutaneous intervention, we reconstructed the biocompatible eluting-freeform coating containing 40 nm fluorescent nanoparticles (NPs) by means of RFP printer and we tested the in-vivo feasibility. We introduced the NPs-loaded 5D device in a rat's vena cava. The coating dissolved in a few minutes releasing NPs which were rapidly absorbed in vascular smooth muscle cell (VSMC) and human umbilical vein endothelial cell (HUVEC) in-vitro. We developed 5D highresolution self-dissolving devices incorporating NPs with the perspective to apply this method to the personalised medicine.Chronic lower extremity peripheral arterial disease (PAD) is a manifestation of systemic atherosclerosis, and one of the main causes in loss of walking ability. Two treatments for lower extremity PAD are currently available: surgical (primarily bypass surgery) and endovascular treatment (EVT) mainly through balloon angioplasty. In fact, due to its clear advantages at least in the short-term, EVT represents -at present -the most common treatment for PAD 1 . Drug-Coated Balloons are made up of a semi-compliant or non-compliant balloon catheter covered with an anti-proliferative agent (typically paclitaxel), and an excipient (e.g. urea) to facilitate drug transfer into the vessel wall after balloon inflation 2 . The BASIL (Bypass Versus Angioplasty for Severe Ischemia of the Leg) trial is the only multicentre, randomised and controlled trial (RCT) which compared these two treatments 3 . One of the major limitations of this RCT is that current endovascular technologies have not been included 4 . Important advances developed in endovascular technologies address a great variety of anatomic challenges, while current and future efforts are directed towards improving long-term patency rates.The 3D printing techniques demonstrate the high potentiality of interactive processes applied to medicine and associated toxicity/vitality studies 5,6 . Materials selected for bio-printed scaffolds are predominantly based on both naturally derived polymers (including gelatine, collagen, alginate, chitosan and hyaluronic acid) or synthetic molecules (polyethylene glycol) 7-9 , allowing precise control 10 of chemical and physical properties 11 .

show abstract

Section: Resultsmentioning

confidence: 99%

In-vivo vascular application via ultra-fast bioprinting for future 5D personalised nanomedicine

Foresti

Rossi

Pinelli

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Cryogelation is a complex process and consists of three steps: non-deep freezing of a polymer solution, its storage in the frozen state, and thawing. The obtained cryogels are characterized by elasticity and a soft, porous structure with high permeability for drug molecules [11,12]. Tentative tests comparing the effect of solvent-casting and freeze-thaw method on placebo film characteristics showed that films formulated by freeze-thawing possessed significantly better mechanical, swelling, and mucoadhesive properties (data presented in Supplementary Materials).…”

Section: Resultsmentioning

confidence: 96%

“…Solvent crystallization during freezing might lead the polymer chains to connect into structures with a large number of macropores. ALG gels obtained using freeze-thaw method demonstrate a significant shrinkage that indicates a high degree of a balance between repulsive and attractive interactions, which, as a consequence, might lead to the creation of junction zones through Van der Waals forces and between uncharged ALG hydrogen bonds chains in the unfrozen liquid phase during thawing [10,11]. In our work, the OLG effect on the pharmaceutical characteristics of ALG buccal films was also examined.…”

Section: Introductionmentioning

confidence: 90%

Alginate Oligosaccharides Affect Mechanical Properties and Antifungal Activity of Alginate Buccal Films with Posaconazole

et al. 2019

View full text Add to dashboard Cite

Sodium alginate and its oligosaccharides through potential antifungal properties might improve the activity of antifungal drugs enhancing their efficacy and potentially reducing the frequency of application. Mucoadhesive buccal films are oral dosage forms designed for maintaining both local or systemic drug effects and seem to be a very promising alternative to conventional oral formulations. Hence, in this study, mucoadhesive buccal films based on the alginate and its oligosaccharide oligomer composed predominantly of mannuronic acid for the administration of posaconazole-antifungal drug from the azole group were developed. As the polymer gelation method, a relatively new freeze-thaw technique was chosen. All prepared formulations were examined for pharmaceutical tests, swelling, mechanical, and mucoadhesive properties. In addition, the influence of sodium alginate (ALG) and alginate oligosaccharides (OLG) on POS antifungal activity on Candida species was performed. It was observed that film formulation containing 1% ALG and 1% OLG (F2) was characterized by optimal mucoadhesive and swelling properties and prolonged drug release up to 5 h. Additionally, it was shown that OLG affected the growth reduction of all tested Candida spp. The obtained data has opened the way for future research for developing OLG-based dosage forms, which might increase the activity of antifungal drugs.

show abstract

“…Because amine groups in CS are protonated, repulsive electrostatic forces appear among different chains. Therefore, no attractive electrostatic interaction among polyelectrolytes takes part, and hydrogen bonding among chains becomes the main force, determining the gelation of the alginate [44,45], as well as the interaction among PEG and poloxamer with polyelectrolytes. Nevertheless, given that under high ionic strength conditions no aggregation was observed, despite the screening of charges, it is more likely that the denaturation of the BSA, which occurred at pH 2, is the triggering process [46].…”

Section: Discussionmentioning

confidence: 99%

Complex Polysaccharide-Based Nanocomposites for Oral Insulin Delivery

et al. 2020

View full text Add to dashboard Cite

Polyelectrolyte nanocomposites rarely reach a stable state and aggregation often occurs. Here, we report the synthesis of nanocomposites for the oral delivery of insulin composed of alginate, dextran sulfate, poly-(ethylene glycol) 4000, poloxamer 188, chitosan, and bovine serum albumin. The nanocomposites were obtained by Ca2+-induced gelation of alginate followed by an electrostatic-interaction process among the polyelectrolytes. Chitosan seemed to be essential for the final size of the nanocomposites and there was an optimal content that led to the synthesis of nanocomposites of 400–600 nm hydrodynamic size. The enhanced stability of the synthesized nanocomposites was assessed with LUMiSizer after synthesis. Nanocomposite stability over time and under variations of ionic strength and pH were assessed with dynamic light scattering. The rounded shapes of nanocomposites were confirmed by scanning electron microscopy. After loading with insulin, analysis by HPLC revealed complete drug release under physiologically simulated conditions.

show abstract

Non-Conventional Methods for Gelation of Alginate

Cited by 108 publications

References 61 publications

In-vivo vascular application via ultra-fast bioprinting for future 5D personalised nanomedicine

In-vivo vascular application via ultra-fast bioprinting for future 5D personalised nanomedicine

Alginate Oligosaccharides Affect Mechanical Properties and Antifungal Activity of Alginate Buccal Films with Posaconazole

Complex Polysaccharide-Based Nanocomposites for Oral Insulin Delivery

Contact Info

Product

Resources

About