Non‐cross‐reactivity of antibodies to murine LDH‐C4 with LDH‐A4 and LDH‐B4

Liang, Zhiting; Shelton, Joyce A.; Goldberg, Erwin

doi:10.1002/jez.1402400312

Cited by 15 publications

(6 citation statements)

References 22 publications

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“…Development of a human contraceptive vaccine relies on the observation that antibodies to LDHC4 do not cross-react with lactate dehydrogenases of somatic tissues (29,33,34). Antibodies to LDHC4, however, do cross-react with the LDHC4 subunits of different species and these reagents have been useful in mapping antigenic determinants.…”

Section: Discussionmentioning

confidence: 99%

Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence.

Millán

Driscoll

Levan

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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The sequence and structure of human testisspecific L-lactate dehydrogenase [LDHC4, LDHX; (L)-lactate: NAD' oxidoreductase, EC 1.1.1.27] has been derived from analysis of a complementary DNA (cDNA) clone comprising the complete protein coding region of the enzyme. From the deduced amino acid sequence, human LDHC4 is as different from rodent LDHC4 (73% homology) as it is from human LDHA4 (76% homology) and porcine LDHB4 (68% homology).Subunit homologies are consistent with the conclusion that the LDHC gene arose by at least two independent duplication events. Furthermore, the lower degree of homology between mouse and human LDHC4 and the appearance of this isozyme late in evolution suggests a higher rate of mutation in the mammalian LDHC genes than in the LDHA and -P genes.Comparison of exposed amino acid residues of discrete antigenic determinants of mouse and human LDHC4 reveals significant differences. Knowledge of the human LDHC4 sequence will help design human-specific peptides useful in the development of a contraceptive vaccine.

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Section: Discussionmentioning

confidence: 99%

Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence.

Millán

Driscoll

Levan

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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“…Although most of the LDH-C4 is intracellular, a small proportion of LDH-C4 is located on the surface of sperm (Wheat & Goldberg 1977). It is also reported that antibodies to LDH-C4 do not cross-react with its somatic isozymes (Liang et al 1986). With these traits, LDH-C4 is an excellent target for the development of a new kind of immunocontraceptive vaccine.…”

Section: Discussionmentioning

confidence: 99%

Study on the antifertility effects of the plasmid DNA vaccine expressing partial brLDH-C4′

Chang¹,

Peng²,

Yang³

et al. 2006

Reproduction

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Partial cDNA sequence coding for Microtus brandti radde (Brandt's vole) testes-specific lactate dehydrogenase (brLDH-C4) was amplified by reverse transcription-polymerase chain reaction (RT-PCR). By inserting the product into the eukaryotic expression vector pCR3.1, pCR3.1-brLDH-C4 0 was obtained as the prototype of contraceptive DNA vaccine. Immunization with pCR3.1-brLDH-C4 0 in BALB/c mice generated antibodies specific to purified brLDH-C4 0 and native mouse LDH-C4 protein. The birth rate of the pCR3.1-brLDH-C4 0 immunized mice was found to be decreased significantly (80% lower than that of those immunized with pCR3.1). Functions of the elicited antibodies in sera from pCR3.1-brLDH-C4 0 inoculated mice were further explored. The results indicated that the antibodies from the mice injected with pCR3.1-brLDH-C4 0 could cause the agglutination of normal sperm suspension, while the ovarian structure and the development of ovarian follicles of these mice were not impaired, which gives a possible explanation for the immunocontraceptive effects of the pCR3.1-brLDH-C4 0 DNA vaccine.

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“…These findings have provided impetus for development of an immunologic contraceptive of highly purified sperm-specific antigen such as lactate dehydrogenase-C 4 (LDH-C 4 ). 1,2 Antisera against LDH-C 4 are immunospecific and do not react with somatic LDH-A 4 and LDH-B 4 , 3,4 although this was contradicted in another report. 5 Immunization with heterologous LDH-C 4 by passive or active route reduced fertility in rabbits, mice, and baboons, 1,6 although impairment of fertility by allogenic LDH-C 4 is yet to be demonstrated.…”

Section: Introductionmentioning

confidence: 89%

“…Virgin Balb/c female mice in three different groups were immunized with native LDH-C 4 , Glu-LDH-C 4 , and Gossy-LDH-C 4 . Antigens were emulsified in Al(OH) 3 and 50 mg of each Ag were used for primary immunization through i.r. route.…”

Section: Immunization Protocol and Preparation Of Antiseramentioning

confidence: 99%

Newly Exposed Immunochemically Cross‐Reactive Epitopes in Sperm‐Specific LDH After Glucosylation and Gossypol Interaction

Gupta

Syal

2000

American J Rep Immunol

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It is concluded that LDH-C4 is not an immunochemically sperm-specific protein, in which crossreactive epitopes are hidden within its conformation. Due to the large intake of cotton seed (a source of gossypol) by cattle, its unrefined oil by humans in various parts of the world, and the prevelance of diabetic state all the world over, the present study warns of immunological consequences in situ following gossypol interaction and glucosylation of LDH and conformationally related proteins in circulation.

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Non‐cross‐reactivity of antibodies to murine LDH‐C₄ with LDH‐A₄ and LDH‐B₄

Cited by 15 publications

References 22 publications

Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence.

Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence.

Study on the antifertility effects of the plasmid DNA vaccine expressing partial brLDH-C4′

Newly Exposed Immunochemically Cross‐Reactive Epitopes in Sperm‐Specific LDH After Glucosylation and Gossypol Interaction

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