Non-H-2 Antigens Serving as Carrier Determinants in a Primary Anti-Thy-1 Response in Mice

Abstract

The primary immune response of mice to the Thy-1 antigens was elicited by intravenous injection of thymocytes from H-2 compatible donors. The magnitude of the ensuing response judged by the number of plaque forming cells producing antibodies lytic for the Thy-1 bearing cells was influenced by some non-H-2 antigens present on the immunizing thymocytes. Absence of non-H-2 incompatibility resulted in a poor response whereas the presence of such an incompatibility allowed a good response. Genetic analysis revealed that the non-H-2 incompatibility affecting the anti-Thy-1 response is controlled by 1-3 independently segregating genes presumably determing molecules acting as carrier determinants for otherwise poorly immunogenic Thy-1 antigens. The carrier effect appeared to be influenced by the gene dosage of the carrier molecules on the immunizing cells. The data presented are discussed in the context of the current concepts of the mechanisms controlling various immune responses.

Preliminary Analysis of Primary and Secondary Anti-Thy-1 Responses Elicited by Immunization with Cell-Bound and Cell-Free Antigen

“…In the case of the Thy-1 antigen, it has been pro posed that it is recognized by means of associating with either non-H-2 molecules that act as carrier-like moieties [15,16] or by associating with class II mole cules that presumably fulfill the role of carrier [1,2], Therefore, one would expect two different subsets of T cells to be involved.…”