Non‐Invasive Physiology and Pharmacology Using 19F Magnetic Resonance

Jian, Yu; Cui, Weina; Zhao, Dawen; Mason, Ralph P.

doi:10.1016/b978-0-444-53086-8.00005-9

Cited by 19 publications

(27 citation statements)

References 352 publications

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“…In rat thigh muscle, the range of baseline pO 2 values measured by PISTOL and pO 2 response to oxygen challenge were similar to those measured here by the well-established 19 F-MR oximetry technique and those reported previously using 1 H spectroscopy of HMDSO (19) or 19 F MRI of HFB (28), needle electrodes, fiber optical probes (29,30), or electron paramagnetic resonance (31,32). The response of individual voxels and muscles in separate rats to oxygen breathing depends on voxel location (Figs.…”

Section: Discussionsupporting

confidence: 86%

Proton imaging of siloxanes to map tissue oxygenation levels (PISTOL): a tool for quantitative tissue oximetry

et al. 2008

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Hexamethyldisiloxane (HMDSO) has been identified as a sensitive proton NMR indicator of tissue oxygenation (pO 2 ) based on spectroscopic spin-lattice relaxometry. A rapid MRI approach has now been designed, implemented, and tested. The technique, proton imaging of siloxanes to map tissue oxygenation levels (PISTOL), utilizes frequency-selective excitation of the HMDSO resonance and chemical-shift selective suppression of residual water signal to effectively eliminate water and fat signals and pulse-burst saturation recovery 1 H echo planar imaging to map T 1 of HMDSO and hence pO 2 . PISTOL was used here to obtain maps of pO 2 in rat thigh muscle and Dunning prostate R3327 MAT-Lu tumor-implanted rats. Measurements were repeated to assess baseline stability and response to breathing of hyperoxic gas. Each pO 2 map was obtained in 3½ min, facilitating dynamic measurements of response to oxygen intervention. Altering the inhaled gas to oxygen produced a significant increase in mean pO 2 from 55 Torr to 238 Torr in thigh muscle and a smaller, but significant, increase in mean pO 2 from 17 Torr to 78 Torr in MAT-Lu tumors. Thus, PISTOL enabled mapping of tissue pO 2 at multiple locations and dynamic changes in pO 2 in response to intervention. This new method offers a potentially valuable new tool to image pO 2 in vivo for any healthy or diseased state by 1 H MRI.

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Section: Discussionsupporting

confidence: 86%

Proton imaging of siloxanes to map tissue oxygenation levels (PISTOL): a tool for quantitative tissue oximetry

et al. 2008

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“…Direct quantitative methods, including Eppendorf microelectrodes (16), electron paramagnetic resonance (EPR) oximetry (17), 19 F relaxometry (18), or Overhauser enhanced MRI (19), are either invasive or require the injection of a reporter probe, and are currently not clinically applicable. One technique for mapping hypoxia in the current clinical setting is PET using nitroimidazole-derived tracers accumulating within hypoxic areas (20,21).…”

Section: Introductionmentioning

confidence: 99%

Qualification of a Noninvasive Magnetic Resonance Imaging Biomarker to Assess Tumor Oxygenation

Colliez

Neveu

Magat

et al. 2014

Clinical Cancer Research

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Purpose: Although hypoxia has been long recognized as a crucial factor impairing tumor response in many therapeutic schemes, atraumatic and reliable methods of individually quantifying tumor oxygenation are still lacking in day-to-day clinical practice. The aim of this work was to investigate the potentially quantitative properties of our recently described noninvasive magnetic resonance (MR) technique "MOBILE" (mapping of oxygen by imaging lipids relaxation enhancement) and to qualify this endogenous contrast as a tumor hypoxia marker.Experimental Design: The "MOBILE" technique, which assesses the longitudinal MR relaxation rate, R 1 , of lipid protons, was benchmarked with the parent technique which assesses the global (or water) R 1 , in response to a hyperoxic challenge (carbogen breathing) and to a hypoxic challenge (combretastatin A4) in MDA-MB-231 xenografts and in NT2 mammary tumors. Electron paramagnetic resonance (EPR) oximetry was used to quantitatively assess the tumor pO 2 in matching tumors longitudinally.Results and Conclusion: Our study evidenced that (i) positive and negative changes in tumor oxygenation can be detected using MOBILE; (ii) a change in the R 1 of lipids is positively correlated with a change in the tumor pO 2 (P ¼ 0.0217, r ¼ 0.5097); (iii) measured lipid R 1 values are positively correlated with absolute pO 2 values in both tumor models (P ¼ 0.0275, r ¼ 0.3726); and (iv) changes in the R 1 of lipids are more sensitive than changes in the global R 1 . As this technique presents unique translational properties, it seems promising for the individual longitudinal monitoring of tumor oxygenation in a clinical setting.

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“…In addition, the electronegativity can alter ionization of adjacent groups such as carboxyl, phenol, and amine (this is explored further in the next section and presented in Table 2). Several pharmaceuticals, agrochemicals and pesticides include a fluorine atom and have been examined by 19 F NMR as reviewed previously [27, 28]. In other cases 19 F may be added to explore structure activity relationships or the ADMET (Absorption, Distribution, Metabolism, and Excretion Toxicity) process [29].…”

Section: Introductionmentioning

confidence: 99%

“…Notably, some reviews have focused on specific topics such as applications to pharmacology [29, 104], physiology [105], reporter molecules [27, 106–110]. To broaden the utility of 19 F NMR various chemical, physical, and pharmacological innovations have been applied to enhance signal to noise, as discussed in the next section.…”

Section: Introductionmentioning

confidence: 99%