Non-significant Effects of The Geometric Shape of Autologous Cartilage Grafts on Tissue Healing: An Animal Study

Serel, Savaş; Çerkez, Cem; Alpat, Servet Elcin; Yiğit, Polat; Can, Belgin; Göktürk, Hilal

doi:10.1007/s00266-020-01904-x

Cited by 4 publications

(2 citation statements)

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“…Herein, after transplantation, under the joint influence of surgical-induced inflammatory response, subcutaneous and nasal septum pressure, and the new nourishing microenvironment of the nose, 31 a new dynamic equilibrium was reached after grafting as resorption, regeneration, and rearrangement of chondrocytes and type II collagen fibers occurred. In addition, the finding in the current study showed similar changes in the autologous auricular grafts to animal models, 32 but ossification of the grafts could be observed in some animal models, 33 whereas no ossification was observed in this study.…”

Section: Discussionsupporting

confidence: 66%

Biological Changes of Autologous Auricular Cartilage in Rhinoplasty

Sun²,

Sheng³

et al. 2022

Journal of Craniofacial Surgery

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Autologous auricular cartilage is used extensively as a good graft material in rhinoplasty. In this study, clinical specimens from patients who underwent revision rhinoplasty with auricular cartilage grafts were collected to compare the changes before and after auricular cartilage transplantation with the use of histologic, immunohistochemical, and quantitative assays. Patients who underwent revision rhinoplasty from 2018 to 2022 were analyzed. Fresh auricular cartilage left after surgery and auricular cartilage graft tissue were examined and compared. Compared with fresh auricular cartilage, local fibrosis was seen in the transplanted auricular cartilage with a slight decrease in elastic fibers, type II collagen, and extracellular matrix secretion. Quantitative assays showed a decrease in glycosaminoglycan, DNA, and total collagen content in the transplanted auricular cartilage tissue. The results of the study suggest that the histologic characteristics, cell functionality, and biochemical composition of the grafted cartilage changed to a certain extent after autologous auricular cartilage graft rhinoplasty. These results provide insights into the selection of graft/filler materials for rhinoplasty and what changes to expect.

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Section: Discussionsupporting

confidence: 66%

Biological Changes of Autologous Auricular Cartilage in Rhinoplasty

Sun²,

Sheng³

et al. 2022

Journal of Craniofacial Surgery

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“…All specimens stained with H&E were evaluated in terms of chondrocyte proliferation at the incision border of the graft tissue, chondrocyte proliferation at the incision border of the ear cartilage, and the surrounding cartilage integration of the graft. The scoring criteria of Serel et al [16] were modified and used to evaluate the specimens. Chondrocyte proliferation at the incision border of the graft tissue and chondrocyte proliferation at the incision border of the ear cartilage were evaluated according to the criteria in Table 1, and the graft's surrounding cartilage integration was assessed using the criteria in Table 2.…”

Section: Hande Stainingmentioning

confidence: 99%

Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model

Ünlü¹,

Bülbül²,

Kolbaşı³

et al. 2023

Arch Aesthetic Plast Surg

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Background Since cartilage, unlike skin, does not contain vessels, it obtains nutrition by diffusion. This reduces graft viability, resulting in problems such as reductions in size, changes in shape, and resorption of the cartilage graft in the late post-graft period. This study aimed to investigate the effects of adipose-derived mesenchymal cells and conditioned medium (CM) on cartilage graft viability.Methods Dissections were performed 4 months after the injection of 0.5 mL of CM or 2×10<sup>6</sup> mesenchymal stem cells (MSCs) in 0.5 mL after grafting into a control group and two experimental groups (n=21 rabbits in total). Chondrocyte viability and type II collagen expression in the grafted areas were analyzed by hematoxylin-eosin staining and immunohistochemical methods, respectively.Results In the MSC and CM groups, chondrocyte proliferation at the graft tissue incision margin (MSC: P<0.01, CM: P<0.0001), chondrocyte proliferation at the auricular cartilage incision margin (MSC: P<0.05, CM: P<0.0001), integration of the graft with the surrounding cartilage (MSC: P<0.001, CM: P<0.0001) and type II collagen expression levels (MSC: P=0.001, CM: P=0.0002) significantly increased.Conclusions Xenogenic injection of MSCs and CM contributed to new cartilage production without any tumoral effects or immune reactions. In particular, the cell-free nature of CM strengthened its potential for safe use. Since injections of MSC and CM can preserve cartilage graft viability, interest in this technique is expected to increase as long-term results from clinical studies on the subject become available.

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