Non-specific adsorption of serum and cell lysate on 3D biosensor platforms: A comparative study based on SPRi

Abstract

Comparative study for non-specific adsorption of cell lysate and serum on recently developed biosensor surfaces is reported. Different surface chemistries including, polyethylene glycol (PEG), a-cyclodextrin (CD), hydrogel dextran and surface initiated polymerization (SIP) based gold surfaces were taken into account for this study. Various techniques including, surface plasmon resonance imaging (SPRi) and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDIÀTOF/TOF MS) technique to evaluate the surfaces with Fourier transform infrared spectroscopy (FTIR) for the confirmation of surface fabrication were used. A high non-specific adsorption response of cell lysate and serum was observed on these so-called non-fouling surfaces. The obtained results from this comparative study provided some hope to explore SIP and dextran surface as a universal platform for biosensor applications. SIP produced best result and showed high sensitivity and minimum non-specific adsorption. We believe that this

A quadratic isothermal amplification fluorescent biosensor without intermediate purification for ultrasensitive detection of circulating tumor DNA

“…This phenomenon might be attributed to the unavoidable interference caused by the non-specific adsorption of proteins present in serum samples, which adversely affects the performance of the biosensor. 36…”

“…This phenomenon might be attributed to the unavoidable interference caused by the non-specific adsorption of proteins present in serum samples, which adversely affects the performance of the biosensor. 36 We conducted a sample addition recovery experiment to assess the accuracy of our biosensor. Under optimal experimental conditions, we detected three different concentrations of ctDNA (0.1, 1, and 10 pM) in PBS containing 1% human serum.…”

Highly Enhanced Adsorption For The Removal of Hg (II) From Aqueous Solution By Co-Polymerized Al2O3 Nanocomposites Fabricated By Improved Sol-Gel Combustion Route: Synthesis, Adsorption Kinetics & Isotherm Modelling

“…Finally, the precipitate was filtered using a Buchner funnel and washed several timed with distilled water and ethanol to remove monomers, oligomers and excess oxidant until the filtrate become almost colorless. At 80° C the precipitate was dried for 24h [63][64][65][66].…”

“…Al 2 O 3 nanoparticles were coated with different loadings of poly (aniline coanthranilic acid "PANAA") copolymer by using surface-initiated polymerization (SIP) method [63][64][65][66]. 8 ml conc.…”

“…In this work, we focus on the synthesis of poly(aniline co-anthranilic acid)/Al 2 O 3 nanocomposite (PANAA/ Al 2 O 3 ) using the surface initiated polymerization (SIP) method [63][64][65][66]. Various experimental factors influencing the adsorption process along with the isotherm models, kinetics, and thermodynamic investigation have been examined.…”