This context exhaustively investigates the ride comfort performance index on the proposed active suspension vehicle system. Ride comfort in terms of occupants (includes driver and passenger) head acceleration, sprung mass vertical and pitching accelerations is considered. For this examination, a 14-degree-of-freedom human vehicle road integrated system model was extensively developed. Then, an active suspension system composed of a hydraulic actuator and proportional-integral-derivative controller is incorporated into the developed vehicle model to enhance the ride comfort. Besides, the designed controller needs to satisfy other vehicle performance indices like vehicle stability and ride safety. Accordingly, the controller parameters were optimally tunned with the help of genetic algorithm technique, on the basis of integral time absolute error criterion. The objective function was created on the basis of minimizing the integral time absolute error of sprung mass displacement, suspension working space and tire deflection responses. The entire response of human vehicle road integrated model, with the proposed active suspension system and passive suspension system on various random road surfaces (A, B, C, D and E with respect to ISO 8608) with five constant speeds (20, 40, 60, 80 and 100 kmph), was compared via surficial presentation. Furthermore, the comfort measures such as root mean square and vibration dose value from ISO 2631-1 were adopted to evaluate the severity between the occupants via head acceleration response. The simulation results showed that the suggested active suspension system significantly improved the ride comfort with guaranteed vehicle stability and ride safety.