Noncontiguous finished genome sequence and description of Raoultibacter massiliensis gen. nov., sp. nov. and Raoultibacter timonensis sp. nov, two new bacterial species isolated from the human gut

Abstract

As part of the culturomics project aiming at describing the human microbiota, we report in this study the description of the new bacterial genus Raoultibacter gen. nov. that includes two new species, that is , R. massiliensis sp. nov. and R. timonensis sp. nov. The R. massiliensis type strain Marseille‐P2849 T was isolated from the fecal specimen of a healthy 19‐year‐old Saudi Bedouin, while R. timonensis type strain Marseille‐P3277 T was isolated from the feces of an 11‐year‐old pygmy female living in Congo. Strain Marseille‐P2849 T exhibited 91.4% 16S rRNA sequence similarity with Gordonibacter urolithinfaciens , its phylogenetic closest neighbor with standing in nomenclature. As well, strain Marseille‐P3277 T exhibited 97.96% 16S rRNA similarity with strain Marseille‐P2849 T . Both strains were Gram‐positive, motile, nonspore‐forming rod and form transparent microcolonies on blood agar in both anaerobic and microaerophilic atmospheres. The genome sizes of strain Marseille‐P2849 T and strain Marseille‐P3277 T were 3,657,161 bp and 4,000,215 bp, respectively. Using a taxono‐genomic approach combining the phenotypic, biochemical, and genomic characteristics, we propose the genus Raoultibacter gen. nov., which contains strains Marseille‐P2849 T (= CSUR P2849 T , = DSM 103407 T ) and Marseille‐P3277 T (=CCUG 70680 T , =CSUR P3277 T ) as type strains of the species R. massiliensis sp. nov., and R. timonensis sp. nov., respectively.

New members of the family Eggerthellaceae isolated from Marmota himalayana: Xiamenia xianingshaonis gen. nov., sp. nov., from intestinal contents, and Berryella wangjianweii sp. nov., from trachea

“…As shown in , the ability of producing acid (from mannose and raffinose)/inability (from glucose) of strain pair zg-886 T /zg-887 differentiated them from all other strains [6–11, 20, 33–41]; similarly, the ability of strain pair zg-1050 T /zg-1084 to produce acid from glucose differed from their sibling species, Berryella intestinalis 68-1-3 T [6].…”

“…Although C 16 : 0 and C 18 : 1 ω 9 c were the two most abundant CFAs in all novel strains, C 12 : 0 reached a dominant level in zg-1050 T /zg-1084, whereas three different CFAs (C 14 : 0 , iso - C 14 : 0 and C 18 : 0 ) were present at high levels (>10 %) in zg-886 T /zg-887 ( and S4). Moreover, the predominant level of C 18 : 0 in zg-886 T /zg-887 and Berryella intestinalis 68-1-3 T () [6] was in sharp contrast to the modest amount in others (zg-1050 T /zg-1084, Table S4 and Raoultibacter massiliensis Marseille-P2849 T ) [6–9, 20, 33, 34, 36, 42–44].…”

“…nov. [8]. Members of this family are Gram-stain-positive [6][7][8][9], rod-shaped, strictly anaerobic, nonspore-forming and non-haemolytic [8], frequently isolated from human gut or faeces, and occasionally from human oral cavity [10] or bovine rumen [11].…”