Nonlabens aestuariivivens sp. nov., isolated from a tidal flat

Abstract

A Gram-stain-negative, non-flagellated, non-gliding, aerobic and rod-shaped bacterium, designated strain OITF-31T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-31T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain OITF-31T belonged to the genus Nonlabens, clustering with the type strains of Nonlabens dokdonensis and Nonlabens xylanidelens, sharing 98.1 % sequence similarity with each. Strain OITF-31T exhibited 16S rRNA gene sequence similarities of 93.9-95.8 % to the type strains of other Nonlabens species and less than of 91.8 % to the type strains of other recognized species. Strain OITF-31T contained MK-6 as the predominant menaquinone and iso-C17 : 0 3-OH and iso-C15 : 0 as the major fatty acids. The major polar lipids detected in strain OITF-31T were phosphatidylethanolamine, six unidentified lipids and two unidentified aminolipids. The DNA G+C content of strain OITF-31T was 38.1 mol% and its mean DNA-DNA relatedness values with the type strains of N. dokdonensis and N. xylanidelens were 24 and 19 %, respectively. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-31T is separated from recognized species of the genus Nonlabens. On the basis of the data presented, strain OITF-31T is considered to represent a novel species of the genus Nonlabens, for which the name Nonlabens aestuariivivens sp. nov. is proposed. The type strain is OITF-31T (=KCTC 52502T=NBRC 112459T).

Nonlabens ponticola sp. nov., isolated from seawater and reclassification of Nonlabens sediminis as a later heterotypic synonym of Nonlabens tegetincola

“…The genomic DNA G+C contents calculated from the whole genomes of strain MJ115 T and N. antarcticus JCM 14068 T were 40.7 and 38.5 %, respectively, which are within the DNA G+C content range of Nonlabens species [1–10]. The general genomic features of strain MJ115 T and N.…”

“…In addition, it was confirmed that the 16S rRNA gene sequences of strain MJ115 T and N. antarcticus JCM 14068 T obtained by Sanger sequencing and from the genomes were identical. The genomic DNA G+C contents calculated from the whole genomes of strain MJ115 T and N. antarcticus JCM 14068 T were 40.7 and 38.5 %, respectively, which are within the DNA G+C content range of Nonlabens species [1][2][3][4][5][6][7][8][9][10]. The general genomic features of strain MJ115 T and N. antarcticus JCM 14068 T were compared with those of closely related reference strains ( Table 1).…”

“…At the time of writing, the genus Nonlabens comprises 12 valid published species. All of them were isolated from various marine environments such as seawater [5, 6], glacier core [7], seashore sand [8], reclaimed land soil [9], tidal flat [10] and marine organisms [5, 11]. Members of the genus Nonlabens generally feature Gram-negative, orange-pigmented, catalase- and oxidase-positive, rod shaped and contain MK-6 as the major respiratory quinone.…”

“…Growth of strain MJ115 T was examined for 5 days at 30 °C on R2A agar, tryptic soy agar (BD, USA), nutrient agar (BD, USA), marine agar (BD, USA) and laboratory prepared LB agar. Growth of strain MJ115 T at different temperatures (4,10,15,20,25,30,37, 40 and 45 °C) and pH values (5.0-11.0 Table 1. General genomic features of strain MJ115 T and the type strains of closely related Nonlabens species Taxa: 1, strain MJ115 T ; 2, N. marinus S1-08 T ; 3, N. agnitus JCM 17109 T ; 4, N. antarcticus JCM 14068 T .…”

“…Growth of strain MJ115 T was examined for 5 days at 30 °C on R2A agar, tryptic soy agar (BD, USA), nutrient agar (BD, USA), marine agar (BD, USA) and laboratory prepared LB agar. Growth of strain MJ115 T at different temperatures (4,10,15,20,25,30,37,40 at 0.5 pH unit intervals) was evaluated on MA and in MB, respectively, for 5 days. MB with pH 5.0-5.5, pH 6.0-7.0 and pH 8.0-11.0 were prepared using sodium citrate buffer, phosphate buffer and Tris-HCl buffers, respectively [27].…”