Nonlethal dose of silver nanoparticles attenuates TNF-α-induced hepatic epithelial cell death through HSP70 overexpression

Tsai, Tsen Ni; Lee, Tzu Ying; Liu, Maw Shung; Ho, Jia Jing; Huang, Li Ju; Liu, Chia Jen; Chen, Tsan Ju; Yang, Rei Cheng

doi:10.1152/ajpcell.00044.2015

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…A previous study of the protective effect of AgNPs reported the overexpression of HSP70 in Clone 9 cells [22]. Our aim was to obtain more details about the molecular mechanism underlying the protective effect of AgNPs and thus we used a low concentration of AgNPs (5 µg/mL) so as to not induce a cytotoxic effect.…”

Section: Discussionmentioning

confidence: 99%

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

Fehaid

Taniguchi

2018

Science and Technology of Advanced Materials

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Silver nanoparticles (AgNPs) are widely known to have anti-inflammatory properties, but the exact mechanism underlying this anti-inflammatory effect is not clearly understood. Tumor necrosis factor-α (TNFα) is a major pro-inflammatory cytokine that is expressed in the early stage of cell inflammation and induces apoptosis by several known pathways. Our study aimed to investigate the effect of AgNPs on the response of lung epithelial cells to TNFα and the molecular mechanism of this response. Lung epithelial cell line NCI-H292 cells were exposed to AgNPs (5 µg/mL) and/or TNFα (20 ng/mL) for 24 h, then cellular uptake was analyzed using flow cytometry. Our results showed that AgNPs were taken up by cells in a dose-dependent manner and that the cellular uptake ratio of AgNPs was significantly increased in the presence of TNFα. Apoptosis assays indicated that exposure to AgNPs significantly decreased the apoptotic effect of TNFα. Confocal microscopy was used to localize the tumor necrosis factor receptor 1 (TNFR1) and revealed that TNFR1 localized on the surface of cells exposed to TNFα. In contrast, TNFR1 localized inside cells exposed to both AgNPs and TNFα, with very few receptors scattered on the cell membrane. The results indicated that AgNPs reduced the cell surface TNFR1 expression level. The results suggested that the reduction of surface TNFR1 reduced cellular response to TNFα, resulting in an anti-apoptotic effect.

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Section: Discussionmentioning

confidence: 99%

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

Fehaid

Taniguchi

2018

Science and Technology of Advanced Materials

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“…Eryılmaz et al also demonstrated that silver nanoparticles increased βcatenin expression in zebrafish embryos (Eryilmaz et al, 2018). It is still unclear, nevertheless, how silver nanoparticles affect TNF-α expression in the research that are currently being conducted; some discover a favorable correlation (Semedo et al, 2022), while others detect a negative correlation (Tsai et al, 2015). It is worth noting that most of the current studies focus on the cytotoxicity of silver nanoparticles and very few studies have examined the changes in TNF-α expression in cells when free silver nanoparticles are lowered.…”

Section: Discussionmentioning

confidence: 99%

Ag₂O‐TiO₂‐NTs enhance osteogenic activity in vitro by modulating TNF‐α/β‐catenin signaling in bone marrow‐derived mesenchymal stem cells

Zeng,

Lai,

Zheng

2024

Chem Biol Drug Des

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The toxic effects of nanoparticles‐silver oxide (Ag2O) limited its use. However, loading Ag2O nanoparticles into titanium dioxide (TiO2) nanotubes (Ag2O‐TiO2‐NTs) has more efficient biological activity and safety. The aim of this study was to observe the effect of Ag2O‐TiO2‐NTs on osteogenic differentiation of bone marrow‐derived mesenchymal stem cells (BMSCs) and its mechanism. The enzyme activity of lactate dehydrogenase (LDH) and the expression of RUNX family transcription factor 2 (Runx2), OPN, OCN in BMSCs were detected by quantitative real time polymerase chain reaction. At 14 days of induction, the mineralization ability and alkaline phosphatase (ALP) activity of cells in each group were observed by Alizarin Red S staining and ALP staining. In addition, the protein levels of tumor necrosis factor‐α (TNF‐α) and β‐catenin in BMSCs of each group were observed by western blot. After 14 days of the induction, the mineralization ability and ALP activity of BMSCs in the Ag2O‐TiO2‐NTs group were significantly enhanced compared with those in the Ag2O and TiO2 groups. Western blot analysis showed that the BMSCs in the Ag2O‐TiO2‐NTs group exhibited much lower protein level of TNF‐α and higher protein level of β‐catenin than those in the Ag2O and TiO2 groups.Ag2O‐TiO2‐NTs enhance the osteogenic activity of BMSCs by modulating TNF‐α/β‐catenin signaling.

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Combined biological effects of silver nanoparticles and heavy metals in different target cell lines

Xue

Yun

2021

Environ Sci Pollut Res

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Nonlethal dose of silver nanoparticles attenuates TNF-α-induced hepatic epithelial cell death through HSP70 overexpression

Abstract: RC. Nonlethal dose of silver nanoparticles attenuates TNF-␣-induced hepatic epithelial cell death through HSP70 overexpression.

Cited by 5 publications

References 31 publications

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

Ag₂O‐TiO₂‐NTs enhance osteogenic activity in vitro by modulating TNF‐α/β‐catenin signaling in bone marrow‐derived mesenchymal stem cells

Combined biological effects of silver nanoparticles and heavy metals in different target cell lines

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Nonlethal dose of silver nanoparticles attenuates TNF-α-induced hepatic epithelial cell death through HSP70 overexpression

Abstract: RC. Nonlethal dose of silver nanoparticles attenuates TNF-␣-induced hepatic epithelial cell death through HSP70 overexpression.

Cited by 5 publications

References 31 publications

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

Ag2O‐TiO2‐NTs enhance osteogenic activity in vitro by modulating TNF‐α/β‐catenin signaling in bone marrow‐derived mesenchymal stem cells

Combined biological effects of silver nanoparticles and heavy metals in different target cell lines

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Ag₂O‐TiO₂‐NTs enhance osteogenic activity in vitro by modulating TNF‐α/β‐catenin signaling in bone marrow‐derived mesenchymal stem cells