Nonlinear optical microscopy of articular cartilage

Yeh, Alvin T.; Hammer‐Wilson, Marie J.; Sickle, David C. Van; Benton, Hilary P.; Zoumi, Aikaterini; Tromberg, Bruce J.; Peavy, George M.

doi:10.1016/j.joca.2004.12.007

Cited by 90 publications

(84 citation statements)

References 27 publications

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“…Yeh et al, demonstrated alteration in ECM of advanced degenerative bovine articular cartilage [11]. Jessica et al, demonstrated morphological changes in degenerative equine articular cartilage [12].…”

Section: Introductionmentioning

confidence: 99%

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Kumar

Grønhaug

Davies

et al. 2015

Biomed. Opt. Express

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Abstract:In a synovial joint, the articular cartilage is directly affected during the progression of Osteoarthritis (OA). The characterization of early stage modification in extra-cellular matrix of cartilage is essential for detection as well as understanding the progression of disease. The objective of this study is to demonstrate the potential and capability of nonlinear optical microscopy for the morphological investigation of early stage osteoarthritic cartilage. ICRS Grade-I cartilage sections were obtained from the femoral condyle of the human knee. The surface of articular cartilage was imaged by second harmonic generation and two-photon excited fluorescence microscopy. Novel morphological features like microsplits and wrinkles were observed, which would otherwise not be visible in other clinical imaging modalities (e.g., CT, MRI, ultrasound and arthroscope. The presence of superficial layer with distinct collagen fibrils parallel to the articular surface in 4 specimens out of 14 specimens, indicates that different phases of OA within ICRS Grade-I can be detected by SHG microscopy. All together, the observed novel morphologies in early stage osteoarthritic cartilage indicates that SHG microscopy might be a significant tool for the assessment of cartilage disorder.

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Section: Introductionmentioning

confidence: 99%

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Kumar

Grønhaug

Davies

et al. 2015

Biomed. Opt. Express

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“…Its application to cartilage is complicated by the fact that the individual fibres of type II collagen are finer than those of type I and below the limit of resolution, but valuable information on its organisation in cartilage has been derived by image processing [7,8] and polarization senstive measurements [9]. Elastin shows strong two photon fluorescence (TPF) and this technique has revealed the somewhat unexpected presence of an extensive network of elastin fibres in the surface zone of articular cartilage [10,11]. Whilst SHG and TPF are extremely useful in exploring the architecture of fibrous protein matrices there is a need for more general approaches capable of imaging the distribution of other tissue constituents.…”

Section: Introductionmentioning

confidence: 99%

Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering

et al. 2013

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Stimulated Raman Scattering has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interteritorial matrix. Changes in the cells and pericellular matrix were investigated as a function of depth into the cartilage. Lipid was detected in the pericellular matrix of superficial zone chondrocytes. The spectral profile of lipid droplets within the chondrocytes indicated that they contained predominantly unsaturated lipids. The mineral content has been imaged by using the PO4 3-vibration at 959cm -1 and the CO3 2-vibration at 1070cm -1 . Both changes in cells and mineralization are known to be important factors in the progression of osteoarthritis. SRS enables these to be visualised in fresh unstained tissue and consequently should benefit osteoarthiritis research

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“…However, an SHG signal is barely detectable when scattered from nonlinear media such as heat-denatured or degraded collagen fibers (Theodossiou et al, 2006). Recently, SHG imaging has been used to study collagen fibers in cartilage, hard tissue and some tumors (Brown et al, 2003;Yeh et al, 2005).…”

Section: Effect Of Hsp47 Disruption On Collagen Fiber Formationmentioning

confidence: 99%

“…However, we have no direct evidence of whether Hsp47 is necessary for the molecular maturation of network-forming collagen, FACIT collagen and/or other types of collagen which have transmembrane domains. The recently developed SHG imaging method is applicable for the examination of pathological conditions in which collagen synthesis is abnormally stimulated, such as the fibrillation that occurs during osteoarthritis or the development of certain tumors (Brown et al, 2003;Yeh et al, 2005).…”

Section: Hsp47 Acts As a Molecular Chaperone For Many Types Of Collagmentioning

confidence: 99%

The molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation

Masago

Hosoya

Kawasaki

et al. 2012

Journal of Cell Science

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SummaryHeat shock protein 47 kDa (Hsp47) is considered as a molecular chaperone essential for the correct folding of type I and type IV procollagen in the ER. However, the function of Hsp47 for other types of procollagen and its importance for chondrogenesis have never been elucidated. To examine the function of Hsp47 in cartilage formation and endochondral ossification, we conditionally inactivated the Hsp47 gene in chondrocytes using Hsp47 floxed mice and mice carrying a chondrocyte-specific Col2a1-Cre transgene. Hsp47 conditional null mutant mice died just before or shortly after birth, and exhibited severe generalized chondrodysplasia and bone deformities with lower levels of type II and type XI collagen. Second-harmonic generation (SHG) analysis and electron microscopy revealed the accumulation of misaligned type I collagen molecules in the intervertebral discs and a substantial decrease in type II collagen fibers, respectively. Whole-mount skeletal staining showed no calcified region in the vertebral bodies of sacral vertebrae, and revealed that the endochondral bones were severely twisted and shortened. These results demonstrate that Hsp47 is indispensable for well-organized cartilage and normal endochondral bone formation.

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Nonlinear optical microscopy of articular cartilage

Cited by 90 publications

References 27 publications

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering

The molecular chaperone Hsp47 is essential for cartilage and endochondral bone formation

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