Nonradioactive Techniques for Measurement of In Vitro T-Cell Proliferation: Alternatives to the [<sup>3</sup>H]Thymidine Incorporation Assay

Messele, Tsehaynesh; Roos, M. T. L.; Hamann, Dörte; Koot, M.; Fontanet, Arnaud; Miedema, Frank; Schellekens, P. T. A.; Wit, Tobias F. Rinke de

doi:10.1128/cdli.7.4.687-692.2000

Cited by 49 publications

(29 citation statements)

References 36 publications

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“…T-cell proliferative capacity is an important independent predictor of progression to HIV disease [35,36] and can also serve to monitor immunological improvement after therapy [37,38]. Decreased proliferation of T-cells from HIV-infected individuals has been previously measured in response to in-vitro stimulation with CD3 monoclonal antibody, pokeweed mitogen, alloantigens, and recall antigens [39][40][41][42][43]. The response to phytohemagglutinin (PHA) remains unaffected in the early phases but is significantly reduced later in infection [44,45].…”

Section: Discussionmentioning

confidence: 99%

Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus

Cohly

Asad

Das

et al. 2003

IJMS

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pokeweed mitogen (PWM) were also examined in the presence of AZT and Tm. In the infection assay, T, Tm and Cu individually did not reduce p-24 antigen release or improve cell viability. AZT (5µM) + Tm (800 ng/ml) inhibited infection by 37 % and increased cell numbers by 30%; whereas, Tm (80 ng/ml) inhibited infection by 26% and increased cell number by 60%. In the proliferation assay, lymphocytes from HIV-infected patients showed better inhibition of mitogen responsiveness to Tm (800 ng/ml) when compared to AZT at 5 µM or Tm at 80 ng/ml. Turmerin inhibited HIV-infected T-cell proliferation and, in combination with AZT, decreased T-cell infection and increased cell viability. These data provide evidence suggesting that efficacious anti-HIV therapy may be possible using lower, less toxic doses of AZT in the presence of turmerin.

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Section: Discussionmentioning

confidence: 99%

Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus

Cohly

Asad

Das

et al. 2003

IJMS

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“…Direct visual cell counting using a hemocytometer can be very accurate (Fresheney 1987) but is generally too time-consuming and laborious to be of utility in screening applications. Alternative in vitro assays have been developed to assess cell proliferation such as staining cells, measurement of intracellular enzyme activities, measurement of DNA synthesis or measurement of metabolic activity (Wemme et al 1992;Messele et al 2000). Most of the studies dealing with the immunomodulatory activity of dairy products performed only one of these in vitro assays.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the immunomodulatory activity of cheese extracts by a complete and easy to handle in vitro screening methodology

et al. 2005

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A simple and rapid screening methodology based on the in vitro culture of murine hybridoma and human T-lymphocytes was developed to assess the potential immunomodulatory activity of water-soluble extracts (WSE) from cheese. The two immune cell lines were cultured in microplates with or without cheese WSE. The proliferation and the metabolic activity of cells were monitored at their different growth phases by the BrdU (5-bromo-2'-deoxyuridine) and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide] assays, respectively. WSE from Abondance cheese enhanced DNA synthesis and the metabolic activity of the hybridoma cells (1.2-and 1.3-to 1.6-fold of the control, respectively) and also of the T lymphocytes (1.5-fold of the control) for almost all of the dilutions tested. To evaluate the validity of the results obtained following this screening methodology, hybridoma and T-lymphocyte cells were cultivated in 50 ml flasks: the maximal cell density was increased by about 10-16% in presence of cheese WSE for both cell lines and the antibody production by the hybridoma cells was increased by 50%.

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“…[6]. Expression of CD69 antigen, an early activation marker, by T cells in response to specific and unspecific stimuli reflects functional properties of immunocompetent cells [8]. The efficiency of using this marker in patients with type 1 diabetes mellitus and systemic lupus erythematous was demonstrated.…”

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Evaluation of the expression of early activation marker CD69 by CD8+ lymphocytes in HIV-infected patients

Исаков

Куртасова

2007

Bull Exp Biol Med

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Nonradioactive Techniques for Measurement of In Vitro T-Cell Proliferation: Alternatives to the [³H]Thymidine Incorporation Assay

Abstract: T-cell proliferation is an important in vitro parameter

Cited by 49 publications

References 36 publications

Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus

Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus

Assessment of the immunomodulatory activity of cheese extracts by a complete and easy to handle in vitro screening methodology

Evaluation of the expression of early activation marker CD69 by CD8+ lymphocytes in HIV-infected patients

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Nonradioactive Techniques for Measurement of In Vitro T-Cell Proliferation: Alternatives to the [3H]Thymidine Incorporation Assay

Abstract: T-cell proliferation is an important in vitro parameter

Cited by 49 publications

References 36 publications

Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus

Effect of Antioxidant (Turmeric, Turmerin and Curcumin) on Human Immunodeficiency Virus

Assessment of the immunomodulatory activity of cheese extracts by a complete and easy to handle in vitro screening methodology

Evaluation of the expression of early activation marker CD69 by CD8+ lymphocytes in HIV-infected patients

Contact Info

Product

Resources

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Nonradioactive Techniques for Measurement of In Vitro T-Cell Proliferation: Alternatives to the [³H]Thymidine Incorporation Assay