Normal and pathological human testes express hormone-sensitive lipase and the lipid receptors CLA-1/SR-BI and CD36

Arenas, María Isabel; Lobo, M.V. Toledo; Caso, Enrique; Huerta, Lidia; Paniagua, Ricardo; Martı́n-Hidalgo, Martin A

doi:10.1016/j.humpath.2003.08.015

Cited by 31 publications

(33 citation statements)

References 54 publications

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“…The 15P-1 cell line has been extensively described and is typically used for analysis of phagocytosis (Grandjean et al, 1997;Jabado et al, 2002) and can be considered to be an accurate model. In accordance with the findings reported by others (Arenas et al, 2004;Alessio et al, 1996;Greenwalt et al, 1992), our western blot results support the idea that, depending on cell type, CD36 displays different molecular masses probably corresponding to different glycoforms. In germ cell fractions, only one reactive high molecular mass form of CD36 is observed, Journal of Cell Science 118 (14) interpreted as a nonspecific band by several authors (Arenas et al, 2004), while in testis and the Sertoli-derived cell line several forms can be detected.…”

Section: Discussionsupporting

confidence: 93%

“…In accordance with the findings reported by others (Arenas et al, 2004;Alessio et al, 1996;Greenwalt et al, 1992), our western blot results support the idea that, depending on cell type, CD36 displays different molecular masses probably corresponding to different glycoforms. In germ cell fractions, only one reactive high molecular mass form of CD36 is observed, Journal of Cell Science 118 (14) interpreted as a nonspecific band by several authors (Arenas et al, 2004), while in testis and the Sertoli-derived cell line several forms can be detected. By immunolabelling experiments, we detect CD36 protein only in Sertoli cells in vivo as well as in 15P-1 cells in vitro, thus confirming the absence of CD36 expression in germ cells.…”

Section: Discussionsupporting

confidence: 93%

“…In human, CD36, as well as SR-BI and HSL (hormonesensitive lipase), expression and localization are modified in testis tumours reflecting an essential role for lipids and lipid receptors (Arenas et al, 2004). In mice, a null mutation in CD36 does not seem to affect the fertility of mutant animals but reveals important roles in fatty acid and lipoprotein metabolism (Febbraio et al, 1999;Febbraio et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells

Gillot

Jehl-Piétri

Gounon

et al. 2005

Journal of Cell Science

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The CD36 scavenger receptor is involved in the uptake and transport of fatty acids, as well as the phagocytosis process in macrophages. We show here that the CD36 protein is expressed by Sertoli cells in the seminiferous epithelium, mainly during the stages where phagocytosis takes place. Using a Sertoli-derived cell line, we show that addition of germ cells and residual bodies triggers a re-localization of CD36 from the cytoplasm to the plasma membrane of the cells, while latex beads do not. Moreover, Sertoli cell phagocytosis of germ cells, but not of latex beads, is reduced by the presence of fatty acids in the culture medium. In the testis, CD36 plays a key role in both phagocytosis and lipid recycling, for constant production of mature spermatozoa.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells

Gillot

Jehl-Piétri

Gounon

et al. 2005

Journal of Cell Science

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“…The expression of the testicular form of HSL (HSLtes) in postmeiotic germ cells rescues the male infertility observed in HSLdefi cient mice ( 37 ). These results and the presence of HSL in spermatids ( 4,35 ) revealed the rate-limiting role of HSLtes and the importance of CEH activity in the action of HSL on spermatogenesis and, more specifi cally, on spermiogenesis.…”

mentioning

confidence: 87%

“…HSL is localized to elongated spermatids and Sertoli cells guinea pig and mink testis ( 8,33 ). In the rat, HSL is only located in the seminiferous tubules (Sertoli and spermatogenic cells) ( 34 ), whereas in the normal human testis, HSL is detected in seminiferous tubules and Leydig cells ( 35 ).…”

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confidence: 99%

HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains

Casado

Huerta

Ortiz

et al. 2012

Journal of Lipid Research

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This article is available online at http://www.jlr.org Supplementary key words hormone-sensitive lipase • cholesterol esters • steroidogenesis • spermatogenesis • fertility • sterility Spermatogenesis occurs in a series of proliferative and differentiation stages, which can be subdivided into mitotic, meiotic, and spermatogenic phases. Each phase is characterized by distinct cell types (spermatogonia, spermatocytes, and spermatids, respectively) ( 1, 2 ). Pathologic, experimental, and natural seasonal arrest of spermatogenesis are all associated with increases in lipid droplets ( 3, 4 ), indicating a close relationship between fertility and changes in lipid metabolism during spermatogenesis ( 5 ). A constant supply of cholesterol is required within Leydig cells to serve as precursor for the synthesis of steroid hormones (steroidogenesis) ( 6, 7 ), whereas in the seminiferous tubules, cholesterol is involved in germinal cell differentiation to spermatozoids (spermatogenesis or gametogenesis) ( 8 ).There is also considerable evidence indicating that cholesterol is required for the development of gametes and fertility in both sexes. Disruption of the DHCR24 gene, which encodes the cholesterol biosynthetic enzyme desmosterol reductase, causes infertility in male mice ( 9 ). In addition to cholesterol, sterol Abstract There is a tight relationship between fertility and changes in cholesterol metabolism during spermatogenesis. In the testis, class B scavenger receptors (SR-B) SR-BI, SR-BII, and LIMP II mediate the selective uptake of cholesterol esters from HDL, which are hydrolyzed to unesterifi ed cholesterol by hormone-sensitive lipase (HSL). HSL is critical

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Absorption, Transport, Distribution in Tissues and Bioavailability

Canene‐Adams¹,

Erdman

2009

Carotenoids

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Normal and pathological human testes express hormone-sensitive lipase and the lipid receptors CLA-1/SR-BI and CD36

Cited by 31 publications

References 54 publications

Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells

Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells

HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains

Absorption, Transport, Distribution in Tissues and Bioavailability

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