Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital

Abstract

ObjectiveThis study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity.DesignThis was a prospective cross-sectional study.Patients and methodsA prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongkutklao Hospital from September 2014 to October 2014. Conjunctival and lid margin swabs were obtained from patients before they underwent cataract surgery. These swabs were used to inoculate blood agar and chocolate agar plates for culturing. After growth of the normal flora, the antibiotic sensitivity method using tobramycin, moxifloxacin, levofloxacin, and cefazolin was applied.Main outcome measuresNormal flora of conjunctiva and lid margin, along with its antibiotic sensitivity, from patients who underwent cataract surgery was assessed.ResultsA total of 120 eyes were included in this study, and bacterial isolation rates were identified. Five bacteria from the lid margin were cultured, namely, coagulase-negative staphylococcus (58.33%), Streptococcus spp. (2.5%), Corynebacterium (1.67%), Micrococcus spp. (1.67%), and Staphylococcus aureus (0.83%). Two bacteria from the conjunctiva were cultured, namely, coagulase-negative staphylococcus (30%) and Streptococcus spp. (0.83%). Results of antibiotic sensitivity test showed that all isolated bacteria are sensitive to cefazolin 100%, tobramycin 98.67%, levofloxacin 100%, and moxifloxacin 100%.ConclusionCoagulase-negative staphylococci are the most common bacteria isolated from conjunctiva and lid margin.

The effect of recurrent povidone-iodine usage on conjunctival flora in diabetic patients undergoing intravitreal injections

“…The most common bacterial isolate was coagulase-negative staphylococci (76%, S. epidermidis and S. lugdunensis ). These results are consistent with those in previous reports 1,13,19 that showed coagulase-negative staphylococci to be the most common bacteria isolated from conjunctiva.…”

“…Coagulase-negative staphylococci are the most common bacteria isolated from the conjunctiva and the lid margin. 1 Diabetes mellitus was linked with a higher rate of positive bacterial cultures of the conjunctival sac before cataract surgery. 2 It was also shown that patients with diabetes mellitus have more gram-negative bacterial cultures.…”

Emerging Enterococcus isolates in postoperative endophthalmitis by selection pressure of fluoroquinolones: an 11-year multicenter and experimental study

“…was rarely isolated from normal conjunctiva, while several studies isolated Enterococcus spp. from 2.5% to 5.2% in their cases [23][24][25][26][27]. We observed similar pattern of microorganisms population isolated from normal conjunctiva to the previous studies.…”

Bacterial agents and changes in drug susceptibilities in cases of chronic dacryocystitis, Southern China

“…Previous studies have reported positive detection rates for bacteria in lacrimal secretion samples from chronic dacryocystitis patients of 48.3-96% [5][6][7][8][9][10][11][12][13][14][15], with rates of 20.6-87.0% in normal conjunctival sac secretion samples [16][17][18]. Gram-negative bacteria were found in 12.1-48.6% of lacrimal secretion samples from chronic dacryocystitis patients and 1.7-8.9% of normal conjunctival sac secretions.…”

“…Gram-negative bacteria were found in 12.1-48.6% of lacrimal secretion samples from chronic dacryocystitis patients and 1.7-8.9% of normal conjunctival sac secretions. Similarly, anaerobic bacteria were found in 10.7-18.6% and 0-16.5% of the two types of samples, respectively [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Therefore, the results of the current study are fairly consistent with previous findings, with bacteria detected in 61.9% of the lacrimal duct secretion samples from chronic dacryocystitis patients and 50.9% of the non-infectious eye disease patients (P \ 0.05).…”