Normal seminal plasma could preserve human spermatozoa against cryopreservation damages in Oligozoospermic patients

Abstract

Background Cryopreservation of human spermatozoa has been identified as an efficient procedure to preserve fertility in men before any cancer therapy or surgical infertility treatment. Despite the benefits of the procedure, the deleterious effects of cryopreservation have been proven on sperm structure and function. This study aimed to evaluate seminal plasma effects on human sperm characteristics after cryopreservation, and compare the addition of normozoospermic and oligozoospermic seminal plasma in the prepared oligozoospermic samples. Semen samples were collected from fifty-five oligozoospermic men and the twenty fertile individuals who referred to the infertility center. At first, a semen analysis was carried out on each neat ejaculate, and then some were cryopreserved. The remainder of the semen was divided into two, one for seminal plasma removal and the other for sperm preparation. Then, the prepared spermatozoa were cryopreserved in three groups: one with, and another without the addition of oligozoospermic seminal plasma, and still another with the addition of normal seminal plasma. After thawing, sperm DNA integrity, viability, motility, and morphology were determined. Results The percentages of all parameters were significantly lower after cryopreservation in all groups compared to the fresh sample. However, this reduction was lower in the oligozoospermic samples cryopreserved with normal seminal plasma. Conclusion The results indicated that seminal plasma in oligozoospermic patients could not support sperm against cryo-injuries, an indication likely due to insufficient antioxidants and other protective components in oligozoospermic patients. However, normal seminal plasma could slightly preserve sperm characteristics after cryopreservation in oligozoospermic patients.

Lactobacillus plantarumsecretions may exert a cryoprotective effect on human sperm motility: A prospective in vitro study

“…Intriguingly, after thawing, this study found that sperm motility and DNA integrity were also altered compared to raw semen of non‐normozoospermic participants. Our results are in line with several reports showing that spermatozoa from infertile men are more sensitive to the freeze/thaw process than those from fertile men 14,61–63 …”

“…Our results are in line with several reports showing that spermatozoa from infertile men are more sensitive to the freeze/thaw process than those from fertile men. 14,[61][62][63] A number of factors can make spermatozoa of infertile patients more susceptible to cryodamage. This could be attributed to differences in sperm chromatin composition (e.g., protamine 1, protamine 2, and histones) between fertile and infertile men.…”

Effect of normal seminal plasma replacement on cryopreservation of sperm in patients with non-liquefied semen

“…This is due to the non-liquefaction of the seminal plasma as well as to the lack of related substance conducing to a reduced sperm viability. Normal semen seminal plasma contains endogenous antioxidants and non-enzymatic antioxidants that play a protective role in sperm cryopreservation [14]. Therefore, normal seminal plasma was employed to replace non-lique ed seminal plasma in the current study, when non-lique ed semen was preserved at low temperature.…”

Physiological Benefits of Novel Selenium Delivery via Nanoparticles

“…Another key step in IVF for both humans and animals is semen cryopreservation—a process that necessarily involves repeated freezing and thawing cycles, which manifests the production of damaging ROS and causes oxidative stress which together reduces sperm viability by more than 35% [ 84 ]. Under these conditions, studies have shown that SeNP supplementation markedly improves gamete quality during IVF procedures [ 83 , 85 ].…”