Note: Biochemical samples centrifuged in-flight on drones

Katariya, Mayur; Chung, Dwayne Chung Kim; Minifie, Tristan; Gupta, Harshit; Zahidi, Alifa Afiah Ahmad; Liew, Oi Wah; Ng, Tuck Wah

doi:10.1063/1.5031039

Cited by 4 publications

(1 citation statement)

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“…[23][24][25] Capillary tubes are widely used vessels for holding samples of up to 100 μl in volume. [26][27][28] In this work, a PCR thermal cycling method whereby capillary tubes containing the reaction mixture are moved over spatially controlled heated surfaces is described and demonstrated, and its performance is verified. When subjected to programmed tilt displacements, the capillary tubes are displaced by gravitational force to and from specific holding regions kept at constant temperatures.…”

Section: Introductionmentioning

confidence: 99%

Polymerase chain reaction thermal cycling using the programmed tilt displacements of capillary tubes

Abid

Lin

Ong

et al. 2020

Review of Scientific Instruments

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A thermal cycling method, whereby capillary tubes holding polymerase chain reactions are subjected to programmed tilt displacements so that they are moved using gravity over three spatial regions (I, II, and III) kept at different constant temperatures to facilitate deoxyribonucleic acid (DNA) denaturation, annealing, and extension, is described. At tilt speeds in excess of 0.2 rad/s, the standard deviation of static coefficient of friction values was below 0.03, indicating in sync movement of multiple capillary tubes over the holding platform. The travel time during the acceleration phase and under constant velocity between adjacent regions (I to II and II to III) and distant regions (III to I) was 0.03 s and 0.31 s, respectively. The deviations in temperature did not exceed 0.05 °C from the average at the prescribed denaturing, annealing, and extension temperatures applied. DNA amplification was determined by optical readings, the fluorescence signal was found to increase twofold after 30 thermal cycles, and 1.16 × 106 DNA copies/μl could be detected. The approach also overcomes problems associated with thermal inertia, sample adhesion, sample blockage, and handling of the reaction vessels encountered in the other thermal cycling schemes used.

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