Nouvelles recherches sur l’accolement des plaquettes aux fibres de collagène

Hugues, J; Lapière, Ch. M.

doi:10.1055/s-0038-1654833

Cited by 25 publications

(11 citation statements)

References 12 publications

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“…The parallel reduction in viscosity and loss of activity on heating suggests that the native collagen TIME IN MINUTES FIGURE 5 The effect of dinitrofluorobenzene treatment of collagen on its ability to aggregate platelets. A represents control collagen and B represents dinitrofluorobenzene-treated collagen.…”

Section: Aggregation Of Platelets By Collagenmentioning

confidence: 99%

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Aggregation of platelets by collagen

Wilner¹,

Nossel²,

Leroy³

1968

J. Clin. Invest.

172

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A B S T R A C T In studying some of the properties of collagen responsible for the ability to aggregate platelets it was found that thermal treatment at pH 2.5 of acid-soluble human collagen resulted in a sharp reduction in relative viscosity and platelet aggregating activity at about 350C. The reduction in viscosity is known to be associated with structural transition from triple helical to random coil form and it is postulated that the native structure of collagen is essential for its platelet aggregation effect. Blockage of the free amino groups by deamination, N-acetylation, or treatment with dinitrofluorobenzene resulted in over 90%o reduction in platelet aggregating activity. Addition of cationic proteins to collagen, removal of the negatively charged telopeptides by treatment with pepsin, or acetylation of the free carboxyl groups did not significantly affect the platelet aggregating activity of collagen. On the basis of these findings it is suggested that the free amino groups and specifically the epsilon amino groups of lysine are critical for the platelet aggregating activity of collagen whereas the carboxyl groups are of relatively little importance.

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Section: Aggregation Of Platelets By Collagenmentioning

confidence: 99%

“…Purified collagen (1) and other preparations of collagen (2)(3)(4)(5)(6) have been shown to aggregate platelets. and it has been postulated on the basis of light and electron microscopic studies that vessel wall collagen plays an important role in normal hemostasis by initiating platelet plug formation (6)(7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Aggregation of platelets by collagen

Wilner¹,

Nossel²,

Leroy³

1968

J. Clin. Invest.

172

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“…This method has been described in greater detail elsewhere (53). Platelet survival times were determined using the method of Murphy (23) FIGURE 7 Order parameter of platelet survival curves. 5'Crplatelet disappearance curves vary from linear (zero order) in normal animals to exponential (first order) in animals with highly consumptive A-V cannulae.…”

Section: Discussionmentioning

confidence: 99%

“…Oral administration of dipyridamole and sulfinpyrazone decreased cannula platelet consumption in a dosedependent manner with complete interruption at 20 and 250 ,umol/kg body wt per d (in three divided doses), respectively, whereas oral acetylsalicylic acid INTRODUCTION Exposure of nonendothelialized surfaces to circulating blood initiates platelet and coagulation reactions that result in thrombosis (1)(2)(3)(4)(5)(6)(7). Initially, this process involves platelet adhesion, release of constituents, and aggregation to form an enlarging platelet mass (6)(7)(8)(9)(10).…”

mentioning

confidence: 99%

“…Initially, this process involves platelet adhesion, release of constituents, and aggregation to form an enlarging platelet mass (6)(7)(8)(9)(10). Arterial high shear conditions promote the formation of thrombus that is composed largely of platelets, whereas low shear venous flow conditions generate thrombin and fibrin with entrapment of erythrocytes (8)(9)(10).…”

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confidence: 99%

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Experimental Arterial Thromboembolism in Baboons

Harker¹,

Hanson²,

Kirkman³

1979

J. Clin. Invest.

107

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A B S T R A C T A quantitative primate model of arterial thromboembolism has been characterized with respect to mechanism and usefulness in evaluating modifying variables. The model involved the kinetic measurements of 51Cr-platelets and 125I-fibrinogen consumption by femoral arteriovenous cannulae in chaired baboons.Cannula platelet consumption correlated directly with exposed cannular area for irradiated Silastic and polyurethane (correlation coefficients of 0.940 and 0.901, respectively; P <0.001) and remained steady state for months. Nonirradiated Silastic was only minimally reactive with platelets. Despite increased rates ofplatelet consumption circulating fibrinogen was not measurably destroyed by any of the cannulae tested. Cannula platelet consumption was independent of cannula flow rate, platelet count, heparin anticoagulation, and ancrod defibrinogenation."'In-platelet imaging of irradiated Silastic cannulae demonstrated luminal accumulation and subsequent embolization of irregular platelet masses. When irradiated Silastic cannulae were inserted as extension segments in the renal arteries of four animals the glomerular vessels became progressively occluded with nonfibrin-containing platelet thromboemboli. Nonirradiated Silastic cannulae in control arteries produced no significant vascular occlusion. Because the survival of platelets from animals with consumptive cannulae was not shortened in normal recipient animals we concluded that platelets were either irreversibly removed through thromboembolic consumption or unaffected in their viability.Oral administration of dipyridamole and sulfinpyrazone decreased cannula platelet consumption in a dosedependent manner with complete interruption at 20 and 250 ,umol/kg body wt per d (in three divided doses), respectively, whereas oral acetylsalicylic acid Received for publication 15 January 1979 and in revised form 4 April 1979. (10-330 ,umol/kg per d) had no measurable effect on cannula platelet consumption.We conclude that this primate model simulates arterial thrombotic processes in man and that this model is suitable for the in vivo evaluation of biomaterials and of drugs that modify platelet behavior.

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The Arterial Wall and the Haemostatic Process

Legrand¹,

Drouet²

1989

Diseases of the Arterial Wall

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Nouvelles recherches sur l’accolement des plaquettes aux fibres de collagène

Cited by 25 publications

References 12 publications

Aggregation of platelets by collagen

Aggregation of platelets by collagen

Experimental Arterial Thromboembolism in Baboons

The Arterial Wall and the Haemostatic Process

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