Novel antifungal mechanism of oligochitosan by triggering apoptosis through a metacaspase-dependent mitochondrial pathway in Ceratocystis fimbriata

Li, Tengjie; Xu, Mingyu; Liu, Yuanfang; Zhang, Chunmei; Zhang, Yanhua; Peng, Xue; Li, Zongyun; Qin, Sheng; Xing, Ke

doi:10.1016/j.carbpol.2020.116574

Cited by 27 publications

(24 citation statements)

References 42 publications

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“…After incubation, the cells were digested and centrifuged for collection. The cells were stained with PI and Annexin V–FITC and then analyzed using a CytoFLEX analyzer 22 (Beckman Coulter) (for PI, Ex = 535 nm and Em = 615 nm; Annexin V–FITC, Ex = 488 nm and Em = 525 nm). Cells were then treated again with the drug for apoptosis detection and collected.…”

Section: Methodsmentioning

confidence: 99%

Human small intestine cancer cell membrane‐camouflaged quercetin‐melanin for antibacterial and antitumor activity

et al. 2021

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E. coli has become an important factor that can lead to cancer because of its ability to cause diverse intestinal changes. Nano‐polymer materials provide ideal drug delivery systems for preparing antibacterial and anti‐cancer drugs because of their unique structure, easy modification, and high drug loading. The modified natural melanin has the potential to be an excellent nano‐carrier. By improving the water‐solubility and biocompatibility of the loaded natural drug quercetin, the antibacterial effect of quercetin can be fully played. Here, natural melanin was extracted from frozen squid to synthesize carrier polydopamine (PDA) nanoparticles, and the natural drug quercetin (Q) was modified on the surface of PDA by π‐π bond and covalent bond action to produce melanin‐quercetin (PDA‐Q). We also developed human small intestinal cancer cells (HIC) membrane‐camouflaged melanin‐Quercetin (PDA‐Q) nanoparticles as an anti‐cancer platform in vivo. The potential bacteriostatic mechanism was likely driven by the penetration of PDA‐Q in E. coli cells, damaging the integrity of the membranes of E. coli and inducing cell death. The mice wound experiment and bacteremia model experiment revealed that C@PDA‐Q had a strong inhibitory effect on E. coli in vivo. In addition, the results of the in vitro tumor test also revealed that C@PDA‐Q had strong anti‐tumor activity against HIC cells of human small intestinal cancer, and the IC50 value was 12.3 ± 0.7 μg/ml, which was slightly better than that for cisplatin. As both melanin nanoparticles and HIC membrane are natural biomaterials, the synthesized C@PDA‐Q nano‐polymer material shows great potential for use in anti‐cancer nano‐drug loading.

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Section: Methodsmentioning

confidence: 99%

Human small intestine cancer cell membrane‐camouflaged quercetin‐melanin for antibacterial and antitumor activity

et al. 2021

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“…Moreover, the amount of malondialdehyde significantly increased, thus accelerating the death of B. dothidea . Upregulation of apoptotic genes in the treatment group also accelerated cell death, and DNA may be a direct target of the CHEC, or it may interfere with normal DNA synthesis in the nucleus of B. dothidea and stopping the cell cycle at the G1 stage, which may cause severe cell apoptosis (Li, Zhang, et al, 2020). The results of RNA‐Seq were generally consistent with those of qRT‐PCR, and the sequencing results were feasible.…”

Section: Resultsmentioning

confidence: 99%

Study on the antifungal mechanism of Chinese herbal extract on Botryosphaeria dothidea

Shi

Zhou

XiaoE

et al. 2022

Food Processing Preservation

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Kiwifruit (Actinidia chinensis Planch) is a perennial deciduous vines (Tilahun et al., 2019). Its unique flavor and abundant nutrients, such as high levels of vitamin C, anthocyanins, flavone, dietary fiber, and amino acids (Drevinskas et al., 2017;Liu et al., 2019). It has high edible value and health benefits, including hypolipidemic, cholesterol-lowering, antioxidant and cosmetic properties, etc.

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“…The detection of intracellular ROS accumulation was conducted using the fluorescent probe DCFH‐DA (T. J. Li et al., 2020). The Z. rouxii cells were collected as described in section 2.1 and washed three times using PBS (0.01 mol/L, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

“…Our previous work confirmed that thymol and trans ‐cinnamaldehyde could result in Z. rouxii necrosis by inducing extensive cell membrane damage (Wang, Peng, et al., 2022). In recent years, many studies have found that natural active substance, including chitosan, quercetin, curcumin, perillaldehyde, silybin, beta‐amyrin, and so forth, could cause fungal cell apoptosis (programmed death) through a metacaspase‐dependent mitochondrial pathway (Kwun & Lee, 2020; Kwun et al., 2021; T. J. Li et al., 2020; Tian et al., 2017). Thus, apoptosis has become an important pathway for natural active substance to exert antifungal activity.…”

Section: Introductionmentioning

confidence: 99%

Thymol and trans‐cinnamaldehyde induce apoptosis through a metacaspase‐dependent mitochondrial pathway in food‐spoilage yeast Zygosaccharomyces rouxii

Wang

Peng

Zhu

et al. 2022

Journal of Food Science

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This work aimed for the first time to provide detailed insights into thymol and trans-cinnamaldehyde's mechanisms of action on the food-spoilage yeast Zygosaccharomyces rouxii and offers evidence in favor of the activation of an apoptosis-like phenotype. The action mechanisms of thymol and transcinnamaldehyde were investigated by the measurement of a series of typical apoptotic features using flow cytometer or microplate reader. Moreover, quantitative reverse transcription PCR (QRT-PCR) was performed to investigate the effects of thymol and trans-cinnamaldehyde on the transcription of key regulators of apoptosis in Z. rouxii. The results indicated that the treatment of Z. rouxii with thymol or trans-cinnamaldehyde (minimum inhibitory and subinhibitory concentrations) triggered reactive oxygen species (ROS) accumulation, elevated intracellular Ca 2+ level, and depolarized mitochondrial membrane potential (MMP) coupled with hallmarks of apoptosis including mitochondrial cytochrome c (cyt c) release, metacaspase activation, phosphatidylserine (PS) exposure, and DNA fragmentation. Moreover, thymol or trans-cinnamaldehyde treatment upregulated the transcription of proapoptotic regulators including Yca1, Dnm1, Nuc1, Ndi1, and Mmi1 and downregulated the transcription of antiapoptotic regulators of Fis1 and Cdc48, further confirming the apoptosis induced by thymol and trans-cinnamaldehyde in Z. rouxii. In summary, thymol and transcinnamaldehyde probably induced apoptosis through a metacaspase-dependent mitochondrial pathway in Z. roxuii. These findings provide theoretical support for the development of safe and efficient agents used in food preservation.

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Novel antifungal mechanism of oligochitosan by triggering apoptosis through a metacaspase-dependent mitochondrial pathway in Ceratocystis fimbriata

Cited by 27 publications

References 42 publications

Human small intestine cancer cell membrane‐camouflaged quercetin‐melanin for antibacterial and antitumor activity

Human small intestine cancer cell membrane‐camouflaged quercetin‐melanin for antibacterial and antitumor activity

Study on the antifungal mechanism of Chinese herbal extract on Botryosphaeria dothidea

Thymol and trans‐cinnamaldehyde induce apoptosis through a metacaspase‐dependent mitochondrial pathway in food‐spoilage yeast Zygosaccharomyces rouxii

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