Novel approach to produce biomass-derived oligosaccharides simultaneously by recombinant endoglucanase from Trichoderma reesei

Tao, Yuheng; Yang, Lun; Liu, Yin; Lai, Chenhuan; Huang, Caoxing; Li, Xin; Yong, Qiang

doi:10.1016/j.enzmictec.2019.109481

Cited by 7 publications

(2 citation statements)

References 37 publications

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“…Cellulases of Trichoderma viride were used for pitch control during pulping process in paper and pulp industries (Kumar et al 2018). Recently, Tao et al (2019) reported simultaneous production of xylooligosaccharides (XOS) and cello-oligosaccharides (COS) by recombinant endoglucanase I of T. reesei expressed in P. pastoris, showing a novel application of genetically modified mycozyme for maximum utilisation of natural biomass and fortification of food and feed with functional food ingredients.…”

Section: Cellulasesmentioning

confidence: 99%

Recent Developments in Industrial Mycozymes: A Current Appraisal

Nath

Kango

2021

Mycology

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Fungi, being natural decomposers, are the most potent, ubiquitous and versatile sources of industrial enzymes. About 60% of market share of industrial enzymes is sourced from filamentous fungi and yeasts. Mycozymes (myco-fungus; zymes-enzymes) are playing a pivotal role in several industrial applications and a number of potential applications are in the offing. The field of mycozyme production, while maintaining the old traditional methods, has also witnessed a sea change due to advents in recombinant DNA technology, optimisation protocols, fermentation technology and systems biology. Consolidated bioprocessing of abundant lignocellulosic biomass and complex polysaccharides is being explored at an unprecedented pace and a number of mycozymes of diverse fungal origins are being explored using suitable platforms. The present review attempts to revisit the current status of various mycozymes, screening and production strategies and applications thereof.

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Section: Cellulasesmentioning

confidence: 99%

Recent Developments in Industrial Mycozymes: A Current Appraisal

Nath

Kango

2021

Mycology

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“…Recombinant endoglucanase I (ReEG I) showed catalytic activity in relation to cellulose and xylan hydrolysis. Among several glucan and xylan substrates (paper pulp, carboxymethylated cellulose, oat xylan, birch xylan), birch xylan exhibited higher yield of xylooligosaccharides (XOS) [54].…”

Section: Recombinant Fungal Cellulases Produced By Different Expression Systemsmentioning

confidence: 99%

Recombinant Fungal Cellulases for the Saccharification of Sugarcane Bagasse

Benevides¹,

Assis²,

Vitor³

et al. 2022

Biodegradation Technology of Organic and Inorganic Pollutants

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Cellulases are important enzymes in cellulose degradation that occurs in nature, this degradation involves a system of extracellular multienzymes and have wide application. The construction of a high-quality system for the production of these enzymes is important for its application in the process of saccharification of biomass involved in the biofuel production process. Several species of fungi are capable of synthesizing and secreting high amounts of cellulase, most studies with fungal species use linearized plasmid, since these are encompassed to chromosomal DNA, improving its stability and expression efficiency. Advances in the production of recombinant enzymes focus on the search for industrially viable microorganisms capable of producing enzymes under various conditions, expressing them in a highly efficient manner, aiming at the synthesis of several copies of genes and a strong promoter. To resay these restrictions, molecular biology combined with recombinant DNA technology is a viable tool in enzymatic production. In subsequent topics, the production of endoglucanases, exoglucanases and β-glucosidase of fungi cloned in Escherichia coli, Pichia pastoris and other different expression systems will be addressed.

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