Novel circular RNA circ_0086722 drives tumor progression by regulating the miR-339-5p/STAT5A axis in prostate cancer

Deng, Wen; Zhou, Xiaochen; Zhu, Ke; Chen, Ru; Liu, Xiaoqiang; Chen, Luyao; Jiang, Hao; Hu, Bing; Zeng, Zhenhao; Cheng, Xiaofeng; Yao, Zhijun; Nie, Jianqiang; Xiong, Situ; Zhang, Cheng; Guo, Ju; Fu, Bin; Wang, Gongxian

doi:10.1016/j.canlet.2022.215606

Cited by 24 publications

(14 citation statements)

References 46 publications

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“…For instance, circ-NOLC1 prompted PCa development via interaction with miR-647 [19]. Circ-0086722 drove PCa progression by mediating STAT5A expression by sponging miR-339-5p [20]. Existing evidence suggests a vital action of the circRNA/miRNA/mRNA competitive endogenous RNA (ceRNA) network pathway in PCa [21,22].…”

Section: Introductionmentioning

confidence: 99%

CircTRRAP (hsa_circ_0081234) participates in prostate cancer progression and glycolysis by HOXA1 via functioning as a miR-515-5p sponge

Guan

Teng

et al. 2022

Appl Biol Chem

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Dysregulated circular RNAs (circRNAs) are implicated in prostate cancer (PCa) progression. Hsa_circ_0081234 (circTRRAP) has been revealed as a facilitator in PCa, but the mechanisms associated with circTRRAP in PCa progression are largely unclear. The present study was to explore the regulatory mechanism of circTRRAP-mediated PCa progression. A total of 50 PCa tissues and normal tissues were collected. RNA levels of circTRRAP, microRNA (miR)-515-5p and homeobox A1 (HOXA1) were detected by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Cell viability, proliferation, migration, and invasion were estimated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, 5-ethynyl-2ʹ-deoxyuridine (EdU) and transwell assays, respectively. Cell glycolysis was assessed by measuring glucose uptake and lactate production. The target interaction between circTRRAP or HOXA1 and miR-515-5p was investigated by the dual-luciferase reporter assay. We observed the overt upregulaiton of circTRRAP in PCa samples and cells. Silencing of circTRRAP lowered tumor growth in vivo and restrained PCa cell viability, proliferation, migration, invasion, and glycolysis in vitro. miR-515-5p was negatively regulated by circTRRAP and its deficiency reversed the inhibiting effects of circTRRAP knockdown on PCa cell malignancy and glycolysis. HOXA1 was confirmed as a miR-515-5p target and miR-515-5p overexpression lessened PCa cell malignancy and glycolysis by decreasing HOXA1 expression. Importantly, circTRRAP mediated HOXA1 expression by functioning as a miR-515-5p sponge. In conclusion, circTRRAP took part in PCa progression and glycolysis through mediating the miR-515-5p/HOXA1 axis, suggesting that circTRRAP can serve as a potential therapeutic target for PCa patients.

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Section: Introductionmentioning

confidence: 99%

CircTRRAP (hsa_circ_0081234) participates in prostate cancer progression and glycolysis by HOXA1 via functioning as a miR-515-5p sponge

Guan

Teng

et al. 2022

Appl Biol Chem

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“…It is acknowledged that circRNAs can participate in tumorigenesis and progression via several mechanisms, including sponging microRNAs, interacting with RBPs, protein scaffolding and regulating gene transcription [30,31]. Emerging studies have revealed that the mechanism of miRNA sponging widely exists in various types of tumors, such as ovarian cancer [32], prostate cancer [33], and gastric cancer [34]. In breast cancer, circRNAs have also been regarded as a class of noncoding RNAs related to tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, by qPCR analyses of the expression of circ-TRIO in TNBC patients, we found that high circ-TRIO expression led to a poor prognosis, including poor overall survival and diseasefree survival, indicating that circ-TRIO plays a vital role in patients suffering from TNBC. To further explore the molecular mechanism of circ-TRIO, we first identified the subcellular location of circ-TRIO because it has been reported that the mechanisms of circRNAs are correlated with their intracellular location [33,42,43]. Based on the results of the subcellular fractionation and FISH assays, circ-TRIO was located in the cytoplasm of TNBC cells, indicating that circ-TRIO might have the potential to sponge miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Circ-TRIO promotes TNBC progression by regulating the miR-432-5p/CCDC58 axis

Wang

Yang

et al. 2022

Cell Death Dis

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Numerous studies have shown that circRNAs are aberrantly expressed in various cancers and play a significant role in tumor progression. However, the molecular mechanisms of circRNAs in triple-negative breast cancer (TNBC) remain ambiguous. By intersecting throughput data and qRT-PCR results from tissues and cell lines, circ-TRIO was identified as a potential oncogenic regulator of TNBC. Moreover, circ-TRIO expression was detected in TNBC tissues and was correlated with the recurrence and prognosis of TNBC patients. The circular characteristics of circ-TRIO were verified by RNase R and CHX assays. Functionally, the knockdown of circ-TRIO inhibited the proliferation, migration and invasion of TNBC cells, while the overexpression of circ-TRIO resulted in the opposite impacts. Mechanistically, a dual luciferase reporter assay and RNA immunoprecipitation were performed and indicated that circ-TRIO could combine with miR-432-5p to regulate the expression of coiled-coil domain containing 58 (CCDC58). In summary, our study illustrates that circ-TRIO plays an important role in the progression of TNBC by regulating the miR-432-5p/CCDC58 axis, which could broaden our insight into the underlying mechanisms and provide a novel prognostic marker of TNBC in the clinic.

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“…CircHIPK3 upregulated the MCL1 expression by binding with miR‐193‐3p and the overexpression of MCL1 can be abrogated by circHIPK3 knockdown 123 . Deng et al 124 . revealing the significant clinical potential of circ_0086722 in prostate cancer therapy, and circ_0086722 has been shown to drive prostate cancer development through the miR‐339‐5p/STAT5A axis.…”

Section: Lncrnas and Circrnas In Cancersmentioning

confidence: 99%

“…CircHIPK3 upregulated the MCL1 expression by binding with miR-193-3p and the overexpression of MCL1 can be abrogated by circHIPK3 knockdown. 123 Deng et al 124 revealing the significant clinical potential of circ_0086722 in prostate cancer therapy, and circ_0086722 has been shown to drive prostate cancer development through the miR-339-5p/STAT5A axis. A recent study showed that circ-SOBP inhibited amoeboid migration of prostate cancer cells and inhibited invasion and migration, and miR-141-3p was sponged and the MYPT1/p-MLC2 axis was regulated to participate in the tumor suppressor effect of circSOBP.…”

Section: Prostate Cancermentioning

confidence: 99%

LncRNAs and CircRNAs in cancer

Yin

Lin

et al. 2022

MedComm

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It is well known that noncoding RNAs (ncRNAs) cannot encode proteins, but they can play important regulatory roles in tumors by combining with proteins, RNAs, and DNAs. As more and more studies reveal the important roles and underlying mechanisms of long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) in cancer, their huge application potential in cancer therapy cannot be ignored. For example, lncRNAs can be involved in tumor‐related signal transduction pathways, cell cycle control, DNA damage, epigenetic regulation, and microRNA control. A group of studies confirmed that abnormal expression of lncRNAs can affect cancer progression. Furthermore, as covalently closed continuous circular ncRNAs, many recent studies have shown that circRNAs have regulatory effects and other important biological significances in cancer. Interestingly, circRNAs were found to have translational functions. This has greatly attracted people's attention to circRNAs research. In this review, we introduce the important roles of lncRNAs and circRNAs in some representative cancers, respectively. Furthermore, we focus on the biological functions and important clinical therapeutic implications of lnRNAs and circRNAs in neuroblastoma. Our review also focuses on providing rationale and relevant references for novel biomarkers for neuroblastoma diagnosis, prognosis, and treatment.

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Novel circular RNA circ_0086722 drives tumor progression by regulating the miR-339-5p/STAT5A axis in prostate cancer

Cited by 24 publications

References 46 publications

CircTRRAP (hsa_circ_0081234) participates in prostate cancer progression and glycolysis by HOXA1 via functioning as a miR-515-5p sponge

CircTRRAP (hsa_circ_0081234) participates in prostate cancer progression and glycolysis by HOXA1 via functioning as a miR-515-5p sponge

Circ-TRIO promotes TNBC progression by regulating the miR-432-5p/CCDC58 axis

LncRNAs and CircRNAs in cancer

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