Novel Colorimetric and NIR Fluorescent Probe for Bisulfite/Sulfite Detection in Food and Water Samples and Living Cells Based on the PET Mechanism

Abstract

Despite their status of being widely used as food additives, bisulfite (HSO 3 − )/sulfite (SO 3 2− ) can pose serious health risks when they are excessively added. Therefore, it is vital to develop a new method for detecting HSO 3 − /SO 3 2− in foodstuff. In this paper, a benzopyran-benzothiazole derivative (probe DCA−Btl) with near-infrared emission was designed and synthesized by constructing a "push-pull" electronic system. DCA−Btl can selectively recognize HSO 3 − /SO 3 2− via a colorimetric and fluorescence dual channel in DMF/PBS (1:1, v/v, pH = 8.4), and the emission wavelength of DCA−Btl can reach 710 nm. The fluorescence quenching of DCA−Btl after recognition of HSO 3 − is attributed to the photoinduced electron transfer (PET) process of the adduct DCA−Btl-HSO 3 − as evaluated by the DFT/TD-DFT method. In addition, DCA−Btl has many advantages, including a large Stokes shift (95 nm), good anti-interference ability, and little cytotoxicity. What's more, DCA−Btl has been successfully applied for the detection of HSO 3 − /SO 3 2− in actual water samples and food samples such as sugar, red wine, and biscuits with satisfying results, as well as for fluorescent imaging of HSO 3 − in living MCF-7 cells.

Positional Isomeric Symmetric Dipodal Receptors Dangled with Rotatable Binding Scaffolds: Fluorescent Sensing of Silver Ions and Sequential Detection of l-Histidine and Their Multifarious Applications

“…l -Histidine (His), on the other hand, is one of the 22 naturally occurring amino acids that plays an important function in the human body as a possible neurotransmitter in numerous medical systems and regulates the metal transmission process. − Histidine shortage causes chronic kidney diseases and if present in excess amount causes various diseases, including AIDS, malaria, asthma, and pulmonary disorder. − According to the Food and Agriculture Organization (FAO) and WHO, the daily limit of histidine for human beings is 8 to 12 mg/kg of body weight and 13 mg/L in serum. , To date, numerous studies have been carried out on the detection of histidine, including voltammetry, chromatography, mass spectroscopy, fluorimetry, and electrochemical studies . An imbalance in histidine causes many issues in human systems, and it is very difficult currently to detect and determine histidine, which has become a highly challenging area.…”

“…34,35 To date, numerous studies have been carried out on the detection of histidine, including voltammetry, chromatography, mass spectroscopy, fluorimetry, and electrochemical studies. 36 An imbalance in histidine causes many issues in human systems, and it is very difficult currently to detect and determine histidine, which has become a highly challenging area. Sequential detection of cations and amino acids has attracted the interest of various researchers in recent years due to their high binding affinity and high cost−efficacy ratios.…”