Novel cone-and-plate flow chamber with controlled distribution of wall fluid shear stress

Ye, Chongyang; Ali, Shahid; Sun, Qing; Guo, Mengmeng; Liu, Yixuan; Gao, Yan; Huo, Bo

doi:10.1016/j.compbiomed.2019.01.014

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…A custom-made cone-plate device consisting of a stationary plate underneath a rotating cone (Figure 1A,B) was used to generate FSS to the cells. The cone was fabricated in accordance with hydrodynamics calculation, 40,41 in which a uniform wall FSS field was provided on the cell surface (Figure 1C,D). MSCs were treated with 0, 0.5, or 0.8 Pa FSS for 1 h each time, three times a day, in accordance with the fluid mechanics calculations and experiments (Figure S1).…”

Section: Application Of Fluid Shear Stressmentioning

confidence: 99%

Synergistic effects of fluid shear stress and adhesion morphology on the apoptosis and osteogenesis of mesenchymal stem cells

Jiao

Zhao

et al. 2022

J Biomedical Materials Res

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Mechanical microenvironments, such as characteristics defining mechanical environments and fluid flow play an important role in steering the fate of mesenchymal stem cells (MSCs). However, the synergistic effect of adhesion morphology and fluid flow on the biological behavior of MSCs is seldom investigated. In this article, 0.5 or 0.8 Pa fluid shear stress (FSS) was applied to the MSCs on micropatterned substrates, and the apoptosis and osteogenic differentiation of MSCs were measured by double fluorescent staining. Results showed that the cellular adhesion patterns with low circularity and large area are beneficial to the osteogenic differentiation of individual MSCs. Meanwhile, FSS facilitated osteogenic differentiation of MSCs, as shown by the expression of alkaline phosphatase, osteocalcin, and collagen I. In addition, nuclear transfer of Yes‐associated protein, a transcriptional regulator in MSCs, was enhanced after being exposed to FSS. These results demonstrated the synergistic effects of FSS and adhesion morphology in directing the fate of MSCs, and these effects may be adopted to design bio‐functional substrates for cell transplantation in tissue engineering.

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Section: Application Of Fluid Shear Stressmentioning

confidence: 99%

Synergistic effects of fluid shear stress and adhesion morphology on the apoptosis and osteogenesis of mesenchymal stem cells

Jiao

Zhao

et al. 2022

J Biomedical Materials Res

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“…The CPD also allows for long-term exposure to FSS; therefore, this model is well-suited for studying both the acute and chronic endothelial response (seconds–weeks) [ 52 ]. The system is limited by its low throughput in exposing ECs in a single dish to FSS; however, variations using multiple cones connected to a single motor allow for increased output [ 54 ]. Using this model, it is possible to generate FSS of varying magnitudes and directionality.…”

Section: Cone and Platementioning

confidence: 99%

Mechanobiology of the endothelium in vascular health and disease: in vitro shear stress models

Jackson

Bond

George

2022

Cardiovasc Drugs Ther

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In recent years, there has been growing evidence that vascular pathologies arise in sites experiencing an altered haemodynamic environment. Fluid shear stress (FSS) is an important contributor to vascular homeostasis and regulates endothelial cell (EC) gene expression, morphology, and behaviour through specialised mechanosensitive signalling pathways. The presence of an altered FSS profile is a pathological characteristic of many vascular diseases, with the most established example being the preferential localisation of atherosclerotic plaque development. However, the precise haemodynamic contributions to other vascular pathologies including coronary artery vein graft failure remains poorly defined. To evaluate potential novel therapeutics for the treatment of vascular diseases via targeting EC behaviour, it is important to undertake in vitro experiments using appropriate culture conditions, particularly FSS. There are a wide range of in vitro models used to study the effect of FSS on the cultured endothelium, each with the ability to generate FSS flow profiles through which the investigator can control haemodynamic parameters including flow magnitude and directionality. An important consideration for selection of an appropriate model of FSS exposure is the FSS profile that the model can generate, in comparison to the physiological and pathophysiological haemodynamic environment of the vessel of interest. A resource bringing together the haemodynamic environment characteristic of atherosclerosis pathology and the flow profiles generated by in vitro methods of applying FSS would be beneficial to researchers when selecting the appropriate model for their research. Consequently, here we summarise the widely used methods of exposing cultured endothelium to FSS, the flow profile they generate and their advantages and limitations in investigating the pathological contribution of altered FSS to vascular disease and evaluating novel therapeutic targets for the treatment and prevention of vascular disease.

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“…As in vivo fluid in bone cavities is difficult to directly observe, some in vitro loading devices were constructed to exert fluid flow on cells. Compared with cone-and-plate flow chamber [ 6 – 8 ], parallel-plate flow chamber is widely used to produce wall FSS in vitro on cells due to its advantage of controlling the FSS field [ 9 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Fluid-Solid Coupling Simulation of Wall Fluid Shear Stress on Cells under Gradient Fluid Flow

Zhang

Gao

Huo

2021

Applied Bionics and Biomechanics

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Fluid shear stress (FSS) plays a crucial role for cell migration within bone cavities filled with interstitial fluid. Whether the local wall FSS distribution on cell surface depends on the global gradient FSS of flow field should be clarified to explain our previous experimental observation. In this study, finite element models of discretely distributed or hexagonal closely packed cells adherent on the bottom plate in a modified plate flow chamber with different global FSS gradient were constructed. Fluid-solid coupling simulation of wall fluid shear stress on cells was performed, and two types of data analysis methods were used. The results showed that the profile of local FSS distribution on cell surface coincides with the angle of cell migration determined in the previous study, suggesting that RAW264.7 osteoclast precursors may sense the global FSS gradient and migrate toward the low-FSS region under a high gradient. For hexagonal closely packed cells, this profile on the surface of central cells decreased along with the increase of cell spacing, which may be caused by the higher local FSS difference along the direction of FSS gradient in the regions close to the bottom plate. This study may explain the phenomenon of the targeted migration of osteoclast precursors under gradient FSS field and further provide insights into the mechanism of mechanical stimulation-induced bone remodeling.

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Novel cone-and-plate flow chamber with controlled distribution of wall fluid shear stress

Cited by 10 publications

References 17 publications

Synergistic effects of fluid shear stress and adhesion morphology on the apoptosis and osteogenesis of mesenchymal stem cells

Synergistic effects of fluid shear stress and adhesion morphology on the apoptosis and osteogenesis of mesenchymal stem cells

Mechanobiology of the endothelium in vascular health and disease: in vitro shear stress models

Fluid-Solid Coupling Simulation of Wall Fluid Shear Stress on Cells under Gradient Fluid Flow

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