Novel Invasive Orthotopic Bladder Cancer Model With High Cathepsin B Activity Resembling Human Bladder Cancer

Abstract

The new murine bladder model described resembles human bladder disease, making it a useful tool for studying the molecular mechanisms of tumor progression and metastasis, and assaying antimetastatic and anti-invasive agents.

FGFR3 Down-Regulation is Involved in bacillus Calmette-Guérin Induced Bladder Tumor Growth Inhibition

“…Orthotopic MB49 tumors were developed as described 18 . Next, 100µl saline solution or BCG (2mg/ml) were instilled into the bladder twice a week, for 20 days.…”

Inducible Nitric Oxide Synthase and PPARγ are Involved in Bladder Cancer Progression

“…23 Living organisms of an attenuated Mycobacterium tuberculosis bovis strain (Pasteur 1172 P2 strain) (Instituto Nacional de Producción de Biologicos, Buenos Aires, Argentina) containing 3 ϫ 10 6 cfu/mg were used.…”

“…Thus, we evaluated the expression of iNOS and PPAR␥, and their biological relationship with tumor progression in a recently developed mouse bladder cancer model with different stages of invasion. 23 To clinically validate our murine model we also analyzed protein expression in human bladder tumors.…”

Animal Models for Basic and Preclinical Research in Bladder Cancer

“…Human tumors can be formed by different cell subpopulations with varying ability to metastasize and susceptibility to treatment, depending on the site of inoculation (Fidler, 1986). It has been observed that subcutaneous inoculation of murine MB49 bladder cancer cell lines induces lung metastases, and that inoculation of these cells in the bladder does not (Lodillinsky et al, 2009). Similar observations have been made for human tumors using 253J B-V cells .…”

“…However, hematuria is the hallmark of tumor presence ( Figure 4B). Mice with 1x10 4 or 1x10 5 MB49 cell lines, inoculated by electrocautery, present hematuria about 15 or 9 days post-inoculation, respectively (Lodillinsky et al, 2009). Inoculation of 5x10 3 cells in the bladder previously treated with NO 3 Ag generates hematuria in all mice about 7 days after tumor implantation (Chade et al, 2008).…”