Novel process for immobilizing an enzyme on a bacterial cellulose membrane through repeated absorption

Wu, She‐Huang; Wu, Sian-Ming; Su, Fengmin

doi:10.1002/jctb.4994

Cited by 41 publications

(25 citation statements)

References 29 publications

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“…BC has been also used to immobilize enzymes that may be useful for food applications. Enzymes which have been already successfully immobilized for controlled release from BC include lipase (Wu et al, 2017), laccase (Chen et al, 2015), and lysozyme (Bayazidi et al, 2018).…”

Section: Immobilizer Of Probiotics and Enzymesmentioning

confidence: 99%

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Azeredo

Barud

Farinas

et al. 2019

Front. Sustain. Food Syst.

367

221

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Bacterial cellulose (BC) has been produced for a number of applications, mainly focused on the biomedical area. Although there is a variety of interesting applications of BC for food and food packaging, only a few have been explored to the moment, since the high cost of BC production is usually considered as a limiting factor. On the other hand, several cost-effective culture media have been proposed, contributing to reduce BC production costs. This article overviews the bioprocess conditions that affects BC production and the main possible applications of BC for food and food packaging purposes.

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Section: Immobilizer Of Probiotics and Enzymesmentioning

confidence: 99%

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Azeredo

Barud

Farinas

et al. 2019

Front. Sustain. Food Syst.

367

221

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“…The three-dimensional porous structure of BC provides also high accessibility onto the active site through low diffusion resistance of the substrate [10][11][12]. Different methodologies can be used for an effective enzyme immobilization on BC, namely physical adsorption [11,12], entrapment, covalent binding or cross linking [13][14][15][16][17]. Different methodologies can be used for an effective enzyme immobilization on BC, namely physical adsorption [11,12], entrapment, covalent binding or cross linking [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

“…BC structures are considered excellent supports for enzyme immobilization since they can enhance enzyme stability against pH and temperature changes [11]. Different methodologies can be used for an effective enzyme immobilization on BC, namely physical adsorption [11,12], entrapment, covalent binding or cross linking [13][14][15][16][17]. Wu et al [15] investigated the conditions of glucoamylase immobilization on BC beads, using various immobilization techniques.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound‐assisted swelling of bacterial cellulose

Song

Loureiro

et al. 2017

Engineering in Life Sciences

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Bacterial cellulose (BC) was obtained by static cultivation using commercial BC gel from scoby. BC membranes (oven dried and freeze‐dried) were swelled with 8% NaOH, in the absence and in the presence of ultrasound (US), for 30, 60, and 90 min. The influence of swelling conditions on both physico‐chemical properties and molecules entrapment was evaluated. Considering the highest levels of entrapment, an optimum swelling procedure was established: 8% NaOH for 30 min at room temperature in the presence of US. Native and PEGylated laccase from Myceliophthora thermophila was immobilized on BC membranes and a different catalytic behaviour was observed after immobilization. Native laccase presented activity values similar to published reports (5–7 U/gBC) after immobilization whereas PEGylated enzymes showed much lower activity (1–2 U/gBC). BC swelled membranes are presented herein as a potential support for the preparation of immobilized enzymes for industrial applications, like phenolics polymerization.

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“…11,12 However, as with other enzymes, CALB is susceptible to inactivation (inhibition or denaturation) by physical-chemical actions, such as extreme temperature changes, pH changes, mechanical shock, or interaction with other substances. 13,14 One alternative for avoiding or reducing such inactivation is to immobilize enzymes, 15,16 which enables enzyme recovery and reuse. 17 Our research group, in a previous report, 13 immobilized CALB using polyurethane foam as support material by a confinement method.…”

Section: Introductionmentioning

confidence: 99%

Activation of Candida antarctica lipase B in pressurized fluids for the synthesis of esters

Nyari

Zabot

Zamadei

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND The objective of this work was to evaluate the activation of Candida antarctica lipase B (CALB) in pressurized CO2 or liquefied petroleum gas (LPG) and, thereafter, to evaluate the synthesis of esters using activated CALB as a biocatalyst. Before activation, CALB was immobilized in polyurethane. For the esterification reactions, mechanical or ultrasonic agitations were studied. The stability of CALB after reuse cycles was also evaluated. RESULTS Before activation, the enzymatic activity of immobilized CALB was 789 U g‐1. After activation in pressurized CO2 and LPG, the activities increased to 2486 U g‐1 and 1413 U g‐1, respectively. Consequently, the residual activities of CALB after activation in pressurized CO2 and LPG were 315% and 179%, respectively. For the best condition of reaction under ultrasonic agitation, oleic acid conversion was 51.2% when using non‐activated CALB. Otherwise, oleic acid conversions were 66.5% and 77.1% when using CALB activated in LPG and CO2, respectively. This biocatalyst maintained more than 80% of its residual activity when reused for up to 13 cycles. CONCLUSION Activation of CALB in pressurized CO2 provided enzymatic activities 1.76 times higher than activation in pressurized LPG did. Immobilized CALB had good performance as a stable biocatalyst for esterification reactions under ultrasonic agitation. © 2017 Society of Chemical Industry

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Novel process for immobilizing an enzyme on a bacterial cellulose membrane through repeated absorption

Cited by 41 publications

References 29 publications

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Bacterial Cellulose as a Raw Material for Food and Food Packaging Applications

Ultrasound‐assisted swelling of bacterial cellulose

Activation of Candida antarctica lipase B in pressurized fluids for the synthesis of esters

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