Novel protease from the leaves of edible medicinal plantAster koraiensisNakai with antithrombotic activity: Purification and partial characterization

Abstract

Nakaiase, a novel protease with antithrombotic and anticoagulant properties was purified from the leaves of Aster koraiensis Nakai, an edible medicinal plant. It had a molecular weight of 23 kDa, as confirmed by SDS‐PAGE analysis. The protease was active at 30 °C and pH 7.0. The amidolytic activity of nakaiase was inhibited by EDTA, EGTA, and several metal ions (Ca, Ni, and Zn). Nakaiase inhibited fibrin clot formation and degraded blood clot. It also inhibit activated factor X and thrombin enzymatic activity. In addition, nakaiase prolonged activated partial thromboplastin time and prothrombin time. Further, the protease exerted significant protective effects against thrombin‐induced pulmonary thromboembolism in mice. Nakaiase at a dose of 20 mg/kg was devoid of hemorrhagic activity. These results suggest that nakaiase has similar property to metalloprotease‐like protease, and has potential as a therapeutic agent for thrombosis owing to its antithrombotic properties and lack of hemorrhagic activity. Practical applications Aster koraiensis Nakai, an edible medicinal plant, has long been used as a traditional medicine and healthy food source in Korea because of its pharmacological efficacy. This study provides beneficial information regarding the antithrombotic potential of A. koraiensis Nakai and bioactive enzymes. Our findings clarify the antithrombotic activities of a new purified protease, which is useful in the development of potential natural pharmacological resources and agents for functional food and clinical application.

Antioxidant and antithrombotic properties of Dendropanax morbifera Léveille (Araliaceae) and its ferments produced by fermentation processing

“…As a major cause of death throughout the world, cardiovascular disease is expected to increase its mortality from 17.5 million to 22.2 million in 2030 (World Health Organization, ). Therefore, potential novel antithrombotic agents isolated from plants and natural sources have become favored due to its safety, nutrition, and efficiency (Brennan, Lan, & Brennan, ; Choi, Kim, Park, et al, ; Choi, Kim, Kim, & Kim, ). D. morbifera Léveille (Araliaceae), one of the natural sources which recommended for improvement in blood circulation, has been recently investigated and showed its ability to alleviate thrombotic and coagulant events (Choi, Kim, Park, et al, ).…”

Extract of Aster koraiensis Nakai Leaf Ameliorates Memory Dysfunction via Anti-inflammatory Action

“…Aster koraiensis Nakai (AK), a perennial plant that belongs to the Asteraceae family, grows on the Korean peninsula, and AK leaves have also been approved by the Korea Ministry of Food and Drug Safety as an edible material. AK has been traditionally used as a folk medicine to cure strokes, venom poisoning, wounds, and sputum, according to the text of Donguibogam (described in the 17th century, Korea) [ 23 ]. The inhibitory activity of an Aster genus plant on COX-2 and iNOS was also reported [ 24 ].…”

A Preliminary Open-field Study Investigating Commercial Smart Farming of a Potential Alternative Crop, <i>Aster koraiensis</i>

“…A. koraiensis has attracted a lot of attention due to its high potential as a novel functional food, as well as for its pharmaceutical properties. In Korea, A. koraiensis has been occasionally consumed as a seasonal leafy vegetable, and has also traditionally been used as a folk medicine to control stroke, inflammation, and respiratory diseases such as pneumonia, pertussis, and chronic bronchitis (Ahn, 1998;Choi et al, 2017). It has been demonstrated that the extracts and compounds from A. koraiensis have various biological activities, including antioxidant, antidiabetic, antiangiogenic, antithrombotic, chemopreventive, and hepatoprotective activities (Choi et al, 2017;Jung et al, 2002;Kim et al, 2011;Kim et al, 2016;Kim et al, 2018;Lee et al, 2010;Lee et al, 2012;Sohn et al, 2010).…”

“…In Korea, A. koraiensis has been occasionally consumed as a seasonal leafy vegetable, and has also traditionally been used as a folk medicine to control stroke, inflammation, and respiratory diseases such as pneumonia, pertussis, and chronic bronchitis (Ahn, 1998;Choi et al, 2017). It has been demonstrated that the extracts and compounds from A. koraiensis have various biological activities, including antioxidant, antidiabetic, antiangiogenic, antithrombotic, chemopreventive, and hepatoprotective activities (Choi et al, 2017;Jung et al, 2002;Kim et al, 2011;Kim et al, 2016;Kim et al, 2018;Lee et al, 2010;Lee et al, 2012;Sohn et al, 2010). Together with these pharmacological activities, its extracts exhibit preventive and therapeutic efficacy against glaucoma, hepatocarcinoma, diabetic retinopathy, and diabetic nephropathy both in vitro and in vivo (Kim et al, 2011;Kim et al, 2016;Kim et al, 2017;Kim et al, 2018;Lee et al, 2010;Sohn et al, 2010).…”