Novel report on mutation in exon 3 of myostatin (MSTN) gene in Nilagiri sheep: an endangered breed of South India

Sahu, Amiya Ranjan; Jeichitra,; Rajendran, R.; Amri, R.

doi:10.1007/s11250-019-01873-7

Cited by 13 publications

(7 citation statements)

References 15 publications

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“…By contrast, Soufy et al [48] reported that the first intron was monomorphic, and all samples showed the same genotypes in Sanjabi sheep; similarly, Nada et al [49] reported that all samples of the Egyptian (Barki, Ossimi, and Rahmani) and Saudi (Najdi and Harri) breeds showed the same genotype for exon 3 of the MTSN gene. However, Sahu [50] reported the first variations in exon 3 of the MSTN gene in Nilagiri sheep in South Africa, such as g.5622 G>C. Interestingly, the c.159 A>T mutation showed polymorphism between an A and a T allele in Barki sheep, whereas it was monomorphic (AA) in Ossimi, Rahmani, and Najdi breeds. Similarly, polymorphism at c.173 T>G showed three different genotypes (TT, TG, and GG) in Barki and one genotype (TT) in the other breeds examined.…”

Section: Discussionmentioning

confidence: 99%

Genetic variations in the Myostatin gene affecting growth traits in sheep

et al. 2021

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Background and Aim: Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. Myostatin (MSTN) plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study sought to investigate genetic variation in the first intron of the MSTN gene and the association of variants with growth traits in major sheep breeds in Egypt (Barki, Ossimi, and Rahmani) and Saudi Arabia (Najdi) using polymerase chain reaction (PCR) and sequencing. Materials and Methods: Blood samples were collected, and DNA was extracted from 75 animals. A 386 bp fragment in the first intron of the MSTN gene was amplified using PCR. Polymorphic sites were detected using direct sequencing and then correlated with growth traits using a general linear model. Results: Sequence analysis of the first intron of MSTN gene identified six single-nucleotide polymorphisms (SNPs) in the studied breeds. Four mutual SNPs were determined: c.18 G>T, c.241 T>C, c.243 G>A, and c.259 G>T. In addition, two SNPs c.159 A>T and c.173 T>G were monomorphic (AA and TT, respectively) in the Ossimi, Rahmani, and Najdi breeds and polymorphic in the Barki breed. The association analysis revealed that the c.18 G>T and c.241 C>T significantly associated (p<0.05) with birth weight and average daily weight gain, respectively. Conclusion: Our results strongly support MSTN as a candidate gene for marker-assisted selection in sheep breeding programs. Furthermore, the identified variants may be considered as putative markers to improve growth traits in sheep.

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Section: Discussionmentioning

confidence: 99%

Genetic variations in the Myostatin gene affecting growth traits in sheep

et al. 2021

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“…Concerning different sheep breeds with regard to the MSTN gene, the Dalagh sheep breed (Azari et al, 2012), Zel sheep breed (Dehnavi et al, 2012), Karak- achan sheep breed (Bozhila-Sakova et al, 2016), and the Bulgarian Karnobat Merino sheep breed (Dimitrova et al, 2017) were found to be monomorphic. While the Mehreban sheep breed (Jamshidi et al, 2014), Kordi sheep breed (Akbari et al, 2015), Black-Headed Teleorman sheep breed (Lazar et al, 2016), Nilagiri sheep breed (Sahu et al, 2019), and Awassi sheep breed (Bayraktar, 2020) were found to be polymorphic with only two genotypes, the Sanjabi sheep breed (Soufy et al, 2009), Bulgarian Merino sheep breed (Dimitrova et al, 2016), and Batur sheep breed (Haren et al, 2020) were reported to be polymorphic with three genotypes. While studies indicating the MSTN gene polymorphism reported the mm genotype at a higher frequency than the MM genotype, the findings were consistent with our study.…”

Section: Gene and Genotype Frequencies And Genetic Equilibrium Test R...mentioning

confidence: 99%

Determinatıon of the MSTN/HaeIII gene polymorphism in indigenous Morkaraman sheep

Saygili,

Ozdemir

2023

J Hellenic Vet Med Soc

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The aim of this study was to examine the polymorphic structures of the Myostatin (MSTN) gene locus in the native Morkaraman sheep, and to determine the distribution of genotype and allele frequencies. MSTN/HaeIII gene polymorphisms were identified in DNA isolated from blood samples from 262 Morkaraman sheep used in the study, using the PCR-RFLP method. According to the Hardy-Weinberg genetic balance test, it was observed that the distribution of genotype frequencies was not in balance (P<0.01) in the population studied. The MM, Mm and mm genotype frequencies of the MSTN gene in the population were determined as 11.1%, 62.6% and 26.3%, respectively, the frequencies of the M and m allele was 0.42 and 0.58 respectively. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values. Observed heterozygosity (Ho) calculated for MSTN in the whole population was significantly higher than expected heterozygosity (He) values.While the FIS value was found as -0.312, the FIT value was determined as -0.303. The genotype and allele frequencies determined in terms of MSTN gene polymorphism were sufficient to reveal the genotype diversity of the breed.

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“…The MSTN gene inactivation might have an effect on muscle development and regeneration since MSTN gene deletion in mice resulted in a double muscle phenomenon (hyperplasia or hypertrophy) and a significant increase in muscle mass ( 10 – 12 ). Natural mutations of MSTN , which have an obvious double muscle phenotypic effect were found in cattle ( 13 , 14 ), dogs ( 15 ), sheep ( 16 , 17 ), pig ( 18 ), and humans ( 19 , 20 ). CRISPR/Cas9 system is an efficient genome editing tool and has widely been used for functional gene study.…”

Section: Introductionmentioning

confidence: 99%

Generation of Heritable Prominent Double Muscle Buttock Rabbits via Novel Site Editing of Myostatin Gene Using CRISPR/Cas9 System

Zheng

Zhang

et al. 2022

Front. Vet. Sci.

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Rabbits have been domesticated for meat, wool, and fur production, and have also been cherished as a companion, artistic inspiration, and an experimental model to study many human diseases. In the present study, the muscle mass negative regulator gene myostatin (MSTN) was knocked out in rabbits at two novel sites in exon3, and the function of these mutations was determined in subsequent generations. The prominent double muscle phenotype with hyperplasia or hypertrophy of muscle fiber was observed in the MSTN-KO rabbits, and a similar phenotype was confirmed in the F1 generation. Moreover, the average weight of 80-day-old MSTN-KO rabbits (2,452 ± 63 g) was higher than that of wild-type rabbits (2,393.2 ± 106.88 g), and also the bodyweight of MSTN-KO rabbits (3,708 ± 43.06g) was significantly higher (P < 0.001) at the age of 180 days than wild-type (WT) rabbits (3,224 ± 48.64g). In MSTN-KO rabbits, fourteen rabbit pups from the F1 generation and thirteen from the F2 generation stably inherited the induced MSTN gene mutations. Totally, 194 pups were produced in the F1 generation of which 49 were MSTN-KO rabbits, while 47 pups were produced in the F2 generation of which 20 were edited rabbits, and the ratio of edited to wild-type rabbits in the F2 generation was approximately 1:1. Thus, we successfully generated a heritable double muscle buttocks rabbits via myostatin mutation with CRISPR/Cas9 system, which could be valuable in rabbit's meat production and also a useful animal model to study the development of muscles among livestock species and improve their important economic traits as well as the human muscle development-related diseases.

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Novel report on mutation in exon 3 of myostatin (MSTN) gene in Nilagiri sheep: an endangered breed of South India

Cited by 13 publications

References 15 publications

Genetic variations in the Myostatin gene affecting growth traits in sheep

Genetic variations in the Myostatin gene affecting growth traits in sheep

Determinatıon of the MSTN/HaeIII gene polymorphism in indigenous Morkaraman sheep

Generation of Heritable Prominent Double Muscle Buttock Rabbits via Novel Site Editing of Myostatin Gene Using CRISPR/Cas9 System

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