Novel therapeutic biosensor for indinavir—A protease inhibitor antiretroviral drug

Ignaszak, Anna; Hendricks, Nicolette R.; Waryo, Tesfaye; Songa, Everlyne A.; Jahed, Nazeem; Ngece, Rachel Fanelwa; Al‐Ahmed, Amir; Kgarebe, Boitumelo; Baker, Priscilla; Iwuoha, Emmanuel I.

doi:10.1016/j.jpba.2008.10.025

Cited by 26 publications

(19 citation statements)

References 18 publications

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“…For the CNT-electrode (for Etoposide detection), the current response reported in Figure 5(A) has been measured at an anodic peak potential of around +220 mV. Moreover, we obtained values of detection limits within the therapeutic range for each drug, and also sensitivities that are comparable with the results shown by other authors with CYP-based sensors [6–8,49–54]. …”

Section: Resultssupporting

confidence: 84%

Electrochemical Detection of Anti-Breast-Cancer Agents in Human Serum by Cytochrome P450-Coated Carbon Nanotubes

Baj-Rossi

Micheli

Carrara

2012

Sensors

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We report on the electrochemical detection of anti-cancer drugs in human serum with sensitivity values in the range of 8–925 nA/μM. Multi-walled carbon nanotubes were functionalized with three different cytochrome P450 isoforms (CYP1A2, CYP2B6, and CYP3A4). A model used to effectively describe the cytochrome P450 deposition onto carbon nanotubes was confirmed by Monte Carlo simulations. Voltammetric measurements were performed in phosphate buffer saline (PBS) as well as in human serum, giving well-defined current responses upon addition of increasing concentrations of anti-cancer drugs. The results assert the capability to measure concentration of drugs in the pharmacological ranges in human serum. Another important result is the possibility to detect pairs of drugs present in the same sample, which is highly required in case of therapies with high side-effects risk and in anti-cancer pharmacological treatments based on mixtures of different drugs. Our technology holds potentials for inexpensive multi-panel drug-monitoring in personalized therapy.

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Section: Resultssupporting

confidence: 84%

Electrochemical Detection of Anti-Breast-Cancer Agents in Human Serum by Cytochrome P450-Coated Carbon Nanotubes

Baj-Rossi

Micheli

Carrara

2012

Sensors

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“…This was attributed to substrate inhibition of the CYP3A4 at E2 concentrations beyond 0.55 lM. A similar inhibition of CYP2D6 in a Au/ [36,48,49]. The mechanism for the hydroxylation of E2 in presence of CYP3A4 is given in Fig.…”

Section: Electrochemical Responses Of the Au/cys/3mpa-znse/cyp3a4 Biomentioning

confidence: 77%

3-Mercaptopropionic acid capped ZnSe quantum dot-cytochrome P450 3A4 enzyme biotransducer for 17β-estradiol

Ndangili

Jijana

Baker

et al. 2011

Journal of Electroanalytical Chemistry

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“…Purified CYP3A4 was immobilized on gold electrodes coated with 3-mercapto-1-propenesulfonic acid [69], carbon nanofibers [70] and glassy carbon electrodes modified with poly(diallyldimethy-lammonium chloride [71] or Nafio-cobalt (III) sepulchrate [72], whereas CYP3A4-containing didodecyldimethylammonium bromide vesicles were attached to a platinum disc electrode [73]. Thiolate-coated gold electrodes, in turn, were used for immobilization of hepatic microsomes [74].…”

Section: 2 Experimental Approachesmentioning

confidence: 99%

“…The electrochemically-driven CYP3A4 reactions are sensitive to the substrate concentration and can be inhibited by ketoconazole, cimetidine and diclofenac [69, 71], which enables estimation of the k cat and K m values for the substrate turnover and IC 50 for the inactivators (a concentration of an inhibitor that reduces substrate metabolism by 50 %). Some biosensors were reported to have a rapid response time and ability to detect very low concentrations of drugs and pollutants and, hence, could be utilized for electrochemical detection in biological samples [72, 73]. …”

Section: 2 Experimental Approachesmentioning

confidence: 99%

Current Approaches for Investigating and Predicting Cytochrome P450 3A4-Ligand Interactions

Sevrioukova

Poulos

2015

Advances in Experimental Medicine and Biology

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Cytochrome P450 3A4 (CYP3A4) is the major and most important drug-metabolizing enzyme in humans that oxidizes and clears over a half of all administered pharmaceuticals. This is possible because CYP3A4 is promiscuous with respect to substrate binding and has the ability to catalyze diverse oxidative chemistries in addition to traditional hydroxylation reactions. Furthermore, CYP3A4 binds and oxidizes a number of substrates in a cooperative manner and can be both induced and inactivated by drugs. In vivo, CYP3A4 inhibition could lead to undesired drug-drug interactions and drug toxicity, a major reason for late-stage clinical failures and withdrawal of marketed pharmaceuticals. Owing to its central role in drug metabolism, many aspects of CYP3A4 catalysis have been extensively studied by various techniques. Here, we give an overview of experimental and theoretical methods currently used for investigation and prediction of CYP3A4-ligand interactions, a defining factor in drug metabolism, with an emphasis on the problems addressed and conclusions derived from the studies.

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Novel therapeutic biosensor for indinavir—A protease inhibitor antiretroviral drug

Cited by 26 publications

References 18 publications

Electrochemical Detection of Anti-Breast-Cancer Agents in Human Serum by Cytochrome P450-Coated Carbon Nanotubes

Electrochemical Detection of Anti-Breast-Cancer Agents in Human Serum by Cytochrome P450-Coated Carbon Nanotubes

3-Mercaptopropionic acid capped ZnSe quantum dot-cytochrome P450 3A4 enzyme biotransducer for 17β-estradiol

Current Approaches for Investigating and Predicting Cytochrome P450 3A4-Ligand Interactions

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