Novel TNS3-MAP3K3 and ZFPM2-ELF5 fusion genes identified by RNA sequencing in multicystic mesothelioma with t(7;17)(p12;q23) and t(8;11)(q23;p13)

Panagopoulos, Ioannis; Gorunova, Ludmila; Davidson, Ben; Heim, Sverre

doi:10.1016/j.canlet.2014.12.002

Cited by 22 publications

(12 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, the throughput of RNA‐Seq is much higher, and it is a sequence‐based method which can directly determine the nucleotide sequence and reveal the sequence variations (Beyer et al, ; Nalpas et al, ). It has been shown to be effective at detecting the mutations (Sugarbaker et al, ) and alternatively spliced variants in malignant mesothelioma, a disease highly related to silicosis (Dong et al, ; Panagopoulos et al, , ) and identify pro‐inflammatory gene activation in mesothelial cells (Dragon et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, RNA-seq was performed to analyze the early molecular changes during silica exposure in lung cells within 24 h. In contrast , 2013). It has been shown to be effective at detecting the mutations (Sugarbaker et al, 2008) and alternatively spliced variants in malignant mesothelioma, a disease highly related to silicosis (Dong et al, 2009;Panagopoulos et al, 2013Panagopoulos et al, , 2015 and identify pro-inflammatory gene activation in mesothelial cells .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Profiling of the silica-induced molecular events in lung epithelial cells using the RNA-Seq approach

et al. 2017

View full text Add to dashboard Cite

Silicosis is a prolonged, irreversible and incurable occupational disease, and there is a significant number of newly diagnosed cases every year in Hong Kong. Due to the long latency of the disease, the diagnosis can be missed until detailed clinical examination at a later stage. For a better control of this deadly disease, detailing the pro-inflammatory and fibrotic events in the macrophage would be instrumental in understanding the pathogenesis of the disease and essential for the significant biomarkers discovery. In this in vitro study, human cell line model A549 lung epithelial cells were used. The immediate molecular events underneath the activation of quartz silica polymorphs were followed in a time course of 0, 0.5, 2, 8, 16 and 24 h. The transcriptome library was prepared and subjected to RNA-Seq analysis. Data analysis was performed by pathway analysis tools and verified by real-time PCR. The results showed that triggered genes were mainly found in the immune response and inflammatory pathways. An interesting finding was the association of the DNA-binding protein inhibitor (ID) family in the silica exposure to lung cells. The linkage of ID1, ID2 and ID3 to cancer may rationalize themselves to be the markers indicating an early response of silicosis. However, further studies are required to consolidate the roles of these genes in silicosis. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Profiling of the silica-induced molecular events in lung epithelial cells using the RNA-Seq approach

et al. 2017

View full text Add to dashboard Cite

show abstract

“…However, in cancer tissue, dysregulated expression of ELF5 has been reported, including in leukemia/lymphoma . Regarding the fusion gene, only the ZFPM2‐ELF5 fusion gene in multicystic mesothelioma has been reported . The only similarity between ZNF384 and FLF5 is the DNA binding transcription factor.…”

Section: Discussionmentioning

confidence: 99%

“…(46) Regarding the fusion gene, only the ZFPM2-ELF5 fusion gene in multicystic mesothelioma has been reported. (47) The only similarity between ZNF384 and FLF5 is the DNA binding transcription factor. These results suggest that an EWSR1 fusion with any DNA binding transcription factor in the hematopoietic lineage may be sufficient for leukemogenesis.…”

Section: Discussionmentioning

confidence: 99%

EWSR1/ELF5 induces acute myeloid leukemia by inhibiting p53/p21 pathway

et al. 2016

View full text Add to dashboard Cite

The Ewing sarcoma breakpoint region 1 (EWSR1) gene is known to fuse with various partner genes to promote the development of the Ewing sarcoma family of tumors and other sarcomas. In contrast, the association of EWSR1 chimeric fusion genes with leukemia has rarely been reported. We identified a novel EWSR1‐associated chimeric fusion gene in a patient with acute myeloid leukemia harboring 46, XY, t (11; 22) (p13; q12) karyotype abnormality. The patient was refractory to intensified chemotherapy including hematopoietic stem cell transplantation. Total RNA paired‐end sequencing identified a novel chimeric fusion gene as EWSR1/ELF5, a member of the E26 transformation‐specific transcription factor family. Transduction of EWSR1/ELF5 to NIH3T3 cells induced transformation by attenuating with the p53/p21‐dependent pathway. The injection of EWSR1/ELF5‐transduced NIH3T3 cells into NSG‐SCID mice systematically induced the development of tumors in vivo. These results revealed the oncogenic potency of EWSR1/ELF5.

show abstract

“…Similarly, integrated network analysis of microarray data predicted ZFPM2 as one of the key genes regulated by parathyroid hormone receptor 1 in osteosarcoma [290]. Moreover, ZFPM2-ELF5 was identified by RNA sequencing among the fusion gene transcripts in multicystic mesothelioma [291], whereas a ZFPM2 microdeletion was found in a case of adult Wilms tumor [292]. More recently, a study reported the discovery of a novel lncRNA, ZFPM2 antisense RNA 1 (ZFPM2-AS1) and its critical role in gastric carcinogenesis through p53 pathway attenuation [293].…”

Section: Zfpm2/fog2mentioning

confidence: 91%

Multifaceted Role of PRDM Proteins in Human Cancer

Casamassimi

Rienzo

Zazzo

et al. 2020

IJMS

View full text Add to dashboard Cite

The PR/SET domain family (PRDM) comprise a family of genes whose protein products share a conserved N-terminal PR [PRDI-BF1 (positive regulatory domain I-binding factor 1) and RIZ1 (retinoblastoma protein-interacting zinc finger gene 1)] homologous domain structurally and functionally similar to the catalytic SET [Su(var)3-9, enhancer-of-zeste and trithorax] domain of histone methyltransferases (HMTs). These genes are involved in epigenetic regulation of gene expression through their intrinsic HMTase activity or via interactions with other chromatin modifying enzymes. In this way they control a broad spectrum of biological processes, including proliferation and differentiation control, cell cycle progression, and maintenance of immune cell homeostasis. In cancer, tumor-specific dysfunctions of PRDM genes alter their expression by genetic and/or epigenetic modifications. A common characteristic of most PRDM genes is to encode for two main molecular variants with or without the PR domain. They are generated by either alternative splicing or alternative use of different promoters and play opposite roles, particularly in cancer where their imbalance can be often observed. In this scenario, PRDM proteins are involved in cancer onset, invasion, and metastasis and their altered expression is related to poor prognosis and clinical outcome. These functions strongly suggest their potential use in cancer management as diagnostic or prognostic tools and as new targets of therapeutic intervention.

show abstract

Novel TNS3-MAP3K3 and ZFPM2-ELF5 fusion genes identified by RNA sequencing in multicystic mesothelioma with t(7;17)(p12;q23) and t(8;11)(q23;p13)

Cited by 22 publications

References 30 publications

Profiling of the silica-induced molecular events in lung epithelial cells using the RNA-Seq approach

Profiling of the silica-induced molecular events in lung epithelial cells using the RNA-Seq approach

EWSR1/ELF5 induces acute myeloid leukemia by inhibiting p53/p21 pathway

Multifaceted Role of PRDM Proteins in Human Cancer

Contact Info

Product

Resources

About